Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,817,227 2 bp→CT coding (552‑553/864 nt) yicC → putative RNase adaptor protein YicC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,817,2270TC98.2% 188.1 / ‑4.5 54coding (552/864 nt)yicCputative RNase adaptor protein YicC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (23/30);  minor base . (0/1);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
*NC_0009133,817,2280CT98.2% 173.6 / ‑5.1 54L185L (CTG→TTG) yicCputative RNase adaptor protein YicC
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (23/30);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

AACCCGGTTGATATTCTACGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGGA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG‑TCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACC  >  NC_000913/3816988‑3817426
                                                                                                                                                                                                                                                ||                                                                                                                                                                                                       
agcccGGTTGATATTCTACGCGGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGCCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGaa                                                                                                                                                                                              <  2:256546/249‑1 (MQ=255)
   ccGGTTGATATTCTACGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCt                                                                                                                                                                                           >  1:292365/1‑251 (MQ=255)
        agATATTCTACGCTGGCCGGGCGTGCTGGCAGCCCAGGAGCAGGATCTTGAAGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATACTGCAATGGCAGCGTGAACGCTTGG‑TAGCGAAGCTGGacg                                                                                                                                                                                      >  2:58230/2‑249 (MQ=255)
         gATATTCTACGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGa                                                                                                                                                                                     <  2:83501/251‑1 (MQ=255)
         gATATTCTACGCGGGCCGGGCGGGATGGCAGCCCAGGAGCAGGATCTGGACGCCAGTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTAGTGGCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TAGCGAAGCTGGAAg                                                                                                                                                                                      <  2:173208/250‑1 (MQ=255)
           tatTCTACGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATg                                                                                                                                                                                   >  2:68698/1‑251 (MQ=255)
           tatTCTACGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTAAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGACGATg                                                                                                                                                                                   >  2:107278/1‑251 (MQ=255)
                 aCGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGACCGCTTGG‑TCGCGAAGCTGGAAGATGCTCa                                                                                                                                                                               <  2:126746/249‑1 (MQ=255)
                 aCGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATGCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCa                                                                                                                                                                               <  2:376341/249‑1 (MQ=255)
                      ggCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGGAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGttcag                                                                                                                                                                        <  1:147541/251‑5 (MQ=255)
                        ccGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGTCTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCTTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGttcagtt                                                                                                                                                                      <  1:29951/251‑7 (MQ=255)
                          gggCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAgggtcagttgg                                                                                                                                                                    >  2:101866/1‑242 (MQ=255)
                           ggCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGttcagttgga                                                                                                                                                                   <  2:147233/251‑10 (MQ=255)
                           ggCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGttcagttgg                                                                                                                                                                    >  2:21702/1‑242 (MQ=255)
                             cgagATGGCCGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTGCGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGCAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGGAGCTGGACGATGCTCAGGttcagttggaa                                                                                                                                                                  >  2:54922/4‑240 (MQ=255)
                                          cAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTgg                                                                                                                                                     >  1:309861/1‑250 (MQ=255)
                                           aGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTgg                                                                                                                                                     >  1:52493/1‑249 (MQ=255)
                                            ggAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑TGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGc                                                                                                                                                  <  1:38142/251‑1 (MQ=255)
                                               gCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTg                                                                                                                                                                                          >  1:99614/1‑208 (MQ=255)
                                               gCAGGATCTTGACGCCATGGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTg                                                                                                                                                                                          <  2:99614/208‑1 (MQ=255)
                                                  ggATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCGGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGagt                                                                                                                                            <  2:108216/251‑3 (MQ=255)
                                                        ggACGCCAGTGCCGCTGAAATGCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAAGGGCAGCGTGAACGCTTGA‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGagttggtt                                                                                                                                       <  2:125693/249‑8 (MQ=255)
                                                                  gCCGCTGAAATGCTCGCGGCGCTGGATGGTACGC‑GGAGCTCCTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCAGTGTTCGCGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCGCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGcaca                                                                                                                            <  2:99004/251‑1 (MQ=255)
                                                                   ccGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGagtt                                                                                                                                           <  2:368359/235‑4 (MQ=255)
                                                                   ccGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGagtt                                                                                                                                           >  1:368359/1‑232 (MQ=255)
                                                                        gAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCCGCGTCTGGAAGGCGTAACCGCCGAAGTGGTCAAAGTCAGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAAAGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAAACGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAAt                                                                                                                      >  1:194897/1‑251 (MQ=255)
                                                                         aacgtctcGCTGGGCTGGATGGTACGCTGAA‑CGACTTTATGGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTTATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAAtt                                                                                                                     <  2:141893/247‑1 (MQ=255)
                                                                         aaaTTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAACGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAACCAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAAtt                                                                                                                     >  1:389470/1‑251 (MQ=255)
                                                                                cgcgGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGt                                                                                                                <  1:257778/249‑1 (MQ=255)
                                                                                  cggcgCTGGATGGGACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGTATTTGGTTCTGCTGGCACAACGAATTGACGTTGcc                                                                                                            <  1:119670/251‑1 (MQ=255)
                                                                                   ggcgCTGGATGGTACGCTGAA‑CGACTTTAGTGTCGCGCGCGAAACCGAAGGCCAGGCACTGAAAGCATTGATCGAGCCGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCg                                                                                                           <  2:358158/251‑1 (MQ=255)
                                                                                           aTGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCTGACACTGAAAGCATTGATCGAGCAGAGTCTGGAAGGCGTCACCGCCGACGTGGTCAAAGTCCGCTCCCCTATGCCGGCACTACTGCAATGGAAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCAGAAGAACTg                                                                                                   >  1:293051/1‑251 (MQ=255)
                                                                                            ggttACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTgg                                                                                                  <  1:412192/248‑1 (MQ=255)
                                                                                                  gCTGAA‑CGACTGTATTGTCGCGCGCGAAACCGAAGGCCAGGCACTGAAAGCATTGATCGAGCAGCGGCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑GCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGcc                                                                                            <  2:361613/251‑1 (MQ=255)
                                                                                                      gaactacgttATTGTCGGGGGCGAAACCAAAGGTCGGGCACGGAAAGCATGGATCGATCAGCGGCTGAAAGTCGGCCCCGCCTAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGTGAAGCTGGAAGATGCGCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTTGTTCGGCTGGCACAACTAATGGACGTTGCCGAAGAACTGGGCCGCCTc                                                                                          <  2:275911/242‑1 (MQ=255)
                                                                                                             tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTg                                                                                                                    <  2:48791/217‑1 (MQ=255)
                                                                                                             tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTg                                                                                                                    >  1:48791/1‑217 (MQ=255)
                                                                                                               tATTGTCGCGGGCGAACCCGAAGGTCGGGCATTGAAAGCATTGACCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGGCCGCCTCGAAGCGCAt                                                                                 <  2:52493/250‑1 (MQ=255)
                                                                                                                        gcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAag                                                                          >  2:126338/1‑248 (MQ=255)
                                                                                                                         cgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTAACAGTCCGCTCCCATATGCCGGAAATCCTGCACTGGCAGCGTGAACGCTTGG‑TCGCGTAGCTGGAAGATGCTCCGGTTCAGTTGGACAACAACCGACTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTAGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGAc                                                                      >  2:141969/1‑251 (MQ=255)
                                                                                                                             aaaCCGAAGGTCAGGCACTGAAAGAAGTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGGCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTGGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTac                                                                  <  1:159869/251‑1 (MQ=255)
                                                                                                                                   aaGGTCAGGAACTGAAAGCATTGATCGTGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCAATATGCCGGAAATCCTGCTATGGCAGAGTGAACGCTTGA‑TAGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCAGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGACGAACTGGACCGCCTCGAATCGCATGTTAAAGAGTCGTACAACAtt                                                            >  2:2739/1‑251 (MQ=255)
                                                                                                                                                aaaaGCATTGATCGAGCAGCGCCTGGAAGGGGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACG‑CTGGGTCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCGGGAGCAGGAGTGGGTTCTGCTGGCACAACGAATTGACGGTGCCGAAGAACTGGACCGCCTCGAAGCGCATGGTAAAGAGACGTACAACATTCTGAAGAAAAaag                                               <  1:365611/250‑1 (MQ=255)
                                                                                                                                                   aGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAaaga                                              >  2:165763/1‑249 (MQ=255)
                                                                                                                                                         gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTg                                      >  2:93456/1‑251 (MQ=255)
                                                                                                                                                         gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGGACAACATTCTGAAGAAAAAAGAAGCCGTTg                                      >  1:247497/1‑251 (MQ=255)
                                                                                                                                                          aTCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTgg                                     >  2:430751/1‑251 (MQ=255)
                                                                                                                                                            cgatcagcGTCTGGAAGGCGTCCCCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCGGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGgtc                                   <  2:364683/247‑1 (MQ=255)
                                                                                                                                                                         gAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACGGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGactt                      <  1:101866/251‑4 (MQ=255)
                                                                                                                                                                          aaGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGActtt                     >  1:412741/1‑251 (MQ=255)
                                                                                                                                                                          aaGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCGGCAATGGCAGCGTGACCGCTTGG‑TCGCGAGGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGGGTTGGTTCTGCTGGCACAACGAATGGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGActtt                     <  2:225076/251‑1 (MQ=255)
                                                                                                                                                                           aGGCGTCACCGCCGAAGTGGTCAAAGGCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCGGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTa                    <  1:21702/251‑1 (MQ=255)
                                                                                                                                                                                 cACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTatgatg               <  1:83201/250‑1 (MQ=255)
                                                                                                                                                                                             ggTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAGTTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAAcc  <  1:68698/251‑1 (MQ=255)
                                                                                                                                                                                                                                                ||                                                                                                                                                                                                       
AACCCGGTTGATATTCTACGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGGA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG‑TCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACC  >  NC_000913/3816988‑3817426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑