Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,804,133	C→T	S211F (TCT→TTT)	nrdF →	ribonucleoside‑diphosphate reductase 2 subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,804,133	0	C	T	100.0%	102.3 / NA	33	S211F (TCT→TTT)	nrdF	ribonucleoside‑diphosphate reductase 2 subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/20);  total (13/20)

CGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCC  >  NC_000913/2803898‑2804353                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             cGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGAGGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCGATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGCCAACGtta                                                                                                                                                                                                               <  2:346435/251‑3 (MQ=255)                  tCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCTACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCg                                                                                                                                                                                              >  1:215997/1‑251 (MQ=255)                  tCAGCAACATTATCGCGGGGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGATCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTc                                                                                                                                                                                               <  2:146232/250‑1 (MQ=255)                         cATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGa                                                                                                                                                                                       <  1:197360/251‑1 (MQ=255)                            tATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTGTTTTGTTTTATTCCGGTTTCTGGGTGCCGGTGTATTTTTCCAGCCGAGGAAAGCTGGTCAAGACCGCGGACCTGATCCGTCTGATTATACGCGATGAAGCAGTACACGGTTACTACAGAGGCTATAAATATCAGAAAAACATGGAAAAGGTATTTCTGGGACAAGGTGAAGAGATGAAGAGTGTCGCGTTCGAtt                                                                                                                                                                                     <  2:130253/250‑1 (MQ=255)                             aTCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGAttt                                                                                                                                                                                    <  2:106183/250‑1 (MQ=255)                             aTCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATttg                                                                                                                                                                                   >  1:298990/1‑251 (MQ=255)                                  ggTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCt                                                                                                                                                                              >  2:392326/1‑251 (MQ=255)                                           ccGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAActct                                                                                                                                                                      <  1:340783/250‑1 (MQ=255)                                           acGCTGAAAAAGAAAATCGCCAGGGTGTTTCTTGAATCTTTTTTGTTATATTCCGGTTTCTGTCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAACGATATTTCTGGGACAACGTGAAAAGTTGAAAAGTTTCACCTTCGCTTTTTTGCTGGaaattt                                                                                                                                                                      >  2:309396/2‑246 (MQ=255)                                             gCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACg                                                                                                                                                                   <  1:392326/251‑1 (MQ=255)                                                    aaGAAAATCGCCAGTGGGTTTCTTGAATCTTATTTGTTCTATTCCGGATTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATACGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGCTTTGTTGCTGGAACTCTACGACatcg                                                                                                                                                             >  1:369478/1‑247 (MQ=255)                                                               cAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATaca                                                                                                                                                 <  2:169078/251‑1 (MQ=255)                                                                                  tttttGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGcc                                                                                                                                <  1:185251/249‑1 (MQ=255)                                                                                      tGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGCAAGCTGACCAATCCCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGCCAACGtgaaga                                                                                                                                                                                                            >  2:28200/1‑169 (MQ=255)                                                                                      tGTTCGATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCGGATCCGTCTGATTATCCGCTATGAAGCAGTCCACGGTTACTACATAGTCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGtgaaga                                                                                                                                                                                                            <  1:28200/169‑1 (MQ=255)                                                                                        ttCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAAcccc                                                                                                                        >  1:415616/1‑251 (MQ=255)                                                                                                      cTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGAtg                                                                                                          >  2:80109/1‑251 (MQ=255)                                                                                                             ccGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACATCGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGACACCCCGTGGGCTGACGATGTGAAAGc                                                                                                   >  1:125985/1‑251 (MQ=255)                                                                                                                       tttCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGCCGATGTGAAAGCGTTTCTCTGt                                                                                         >  2:435106/1‑251 (MQ=255)                                                                                                                       tttCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTg                                                                                          <  2:415616/250‑1 (MQ=255)                                                                                                                            aGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACaa                                                                                    <  2:319822/251‑1 (MQ=255)                                                                                                                                  ggAAAGCGGACCAATCCCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCat                                                                              <  2:12850/251‑2 (MQ=255)                                                                                                                                       gCTGACCAATACCGCAGACCTGATCCGTCTGATTAGCCGCGATGAAGCAGTCCACTGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTTGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAgg                                                                         <  1:43208/251‑1 (MQ=255)                                                                                                                                                         ccTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGACTCTGGGCt                                                       <  2:40462/251‑1 (MQ=255)                                                                                                                                                                     gATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCGATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTAt                                           <  2:300863/251‑1 (MQ=255)                                                                                                                                                                           ccGCGATGAAGCAGTACACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCGCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGATCCGTTATTTCCCg                                     <  2:348285/251‑1 (MQ=255)                                                                                                                                                                              cgATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGTCTACGAACCGTTATTTCCCGCAg                                  <  2:39800/251‑1 (MQ=255)                                                                                                                                                                                                 tactacATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATc                <  2:107774/250‑1 (MQ=255)                                                                                                                                                                                                           gCTATAACTATCAGAAAAACATGGAAACGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACg                                                    >  2:266141/1‑204 (MQ=255)                                                                                                                                                                                                           gCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACGCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACg                                                    <  1:266141/204‑1 (MQ=255)                                                                                                                                                                                                             tataCATATCAGAGGAGCATGGATAAGATATTTCTGGGGCAACGTGAAGAGTTGAAGAGGTTAGCCTTCGATTTGTTGCTGGCACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAACGGTTCTCTGTTACAACGCCTATACGGCTTTGATGATTCTGGACTCCGAACCGTTATTTCCCGCAGAAATGGCGAAAGTGAATCCGGCAATCCTcgc   >  2:243052/1‑251 (MQ=255)                                                                                                                                                                                                              ataAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTACGATTTGTTGCTGGAACTCTACGCCACCGAGTTGCAATACACAGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGCATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGGGAATCCGGCAATCCTcgcc  >  1:403044/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             CGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCC  >  NC_000913/2803898‑2804353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑