Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,788,391	C→T	intergenic (‑2554/‑594)	ileY ← / → glaH	tRNA‑Ile/glutarate dioxygenase GlaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,788,391	0	C	T	100.0%	41.5 / NA	13	intergenic (‑2554/‑594)	ileY/glaH	tRNA‑Ile/glutarate dioxygenase GlaH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/10);  total (3/10)

ATGGCCAAGTATTAAAATCAACGACTGAAATTAAAAATTATGATGGGTCCACGCGTGTCGGCGGTGAGGCGTAGCTTAATAAAGGTTGCTCTACCTATCAGCAGCTCTACAATGAATTCCACGCATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCACTCTCCTCATGATGGACTAGTTATAATAAATAAAGGAAACGAAGAAGTTGAAGGTACCTGGCCTAACAAATTGCAACCTGGAATATACAAAAACATGGGGAGTAATAGCGTTAACATTATTATTAATAATACCCGAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCG  >  NC_000913/2788165‑2788628                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              aTGGCCAAGTATTAAAATCAACGACTGAAATTAAAAATTATGATGGGTCCACGCGTGTCGGCGGTGAGGCGTAGCTTAATAAAGGTTGCTCTACCTATCAGCAGCTCTACAATGAATTCCACGCATTATATATAGATAAAGCATGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCATGATGGACTAGTtata                                                                                                                                                                                                                       <  1:190420/251‑1 (MQ=255)                             aaTTAAAAATTATGATGGGTCCACGCGTGTCGGCTGTGAGGCGTAGCTTAATAAAGGTTGCTCTACCTATCAGCAGCTCTACAATTAATTCCACGCATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCGTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCATGATGGACTAGTTATAATAAATAAAGGAAACGAAGAAGTTGAAg                                                                                                                                                                                           <  1:128656/251‑1 (MQ=255)                              aTTAAAAATTATGATGGGTCCCCGCGTGTCGGCGGTGAGGCGTAGCTTAATAAAGGTTGCTCTACCTCTCAGCCGCTCTACAATGAATTCCACGAATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCCTGATGGACt                                                                                                                                                                                                                              >  2:175974/1‑215 (MQ=255)                              aTTAAAAATTATGATGGGTCCACGCGTGTCGGCGGTGAGGCGTAGCTTAATAAAGGTTGCTCTACCTATCAGCAGCTCTACAATGAATTCCACGCATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCATGATGGACt                                                                                                                                                                                                                              <  1:175974/215‑1 (MQ=255)                                    aaTTATGATGGGTCCACGCGTGTCGGCGGTGAGGCGTAGCTTAATAAAGGTTGCTCTACCTATCAGCAGCTCTACAATGAATTCCACGCATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCATGATGGACTAGTTATAATAAATAAAGGAAACGAAGAAGTTGAAGGTACCTg                                                                                                                                                                                    >  1:234140/1‑251 (MQ=255)                                                           cggcggTGAGGCGTAGCTTAATAAAGGTTGCTCTACCTATCAGCAGCTCTACAATGAATTCCACGCATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCATGATGGCCTAGTTATAATAAATAAAGGAAACGAAGAAGTTGAAGGTACCTGGCCTAACAAATTGCAACCTGGa                                                                                                                                                              >  2:315048/1‑250 (MQ=255)                                                                       gTAGCTTAATAAAGGTTGCTCTACCTATCAGCAGCTCTACAATGAATTCCACGCATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCATGATGGACTAGTTATAATAAATAAAGGAAACGAAGAAGTTGAAGGTACCTGGCCTAACAAATTGCAACCTGGAATATACAAAAACa                                                                                                                                                 <  2:379882/251‑1 (MQ=255)                                                                                    ggTTGCTCTACCTATCAGCAGCTCTACAATGAATTCCACGCATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCATGATGGACTAGTTATAATAAATAAAGGAAACGAAGAAGTTGAAGGTACCTGGCCTAACAAATTGCAACCTGGAATATACAAAAACATGGGGAGTAATAg                                                                                                                                    <  1:315820/251‑1 (MQ=255)                                                                                        gCTCTACCTATCAGCAGCTCTACAATGAATTCCACGCATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCATGATGGACTAGTTATAATAAATAAAGGAAACGAAGAAGTTGAAGGTACCTGGCCTAACAAATTGCAACCTGGAATATACAAAAACATGGGGAGTAATAGCGt                                                                                                                                 <  2:343766/250‑1 (MQ=255)                                                                                             aCCTATCAGCAGCTCTACAATGAATTCCACGCATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCATGATGGACTAGTTATAATAAATAAAGGAAACGAAGAAGTTGAAGGTACCTGGCCTAACAAATTGCAACCTGGAATATACAAAAACATGGGGAGTAATAGCGTTaa                                                                                                                              <  2:231907/248‑1 (MQ=255)                                                                                                 aTCAGCAGCTCTACAATGAATTCCACGCATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCATGATGGACTAGTTATAATAAATAAAGGAAACTAAGAAGTTGAAGGTACCTGGCCTAACAAATTGCAACCTGGAATATACAAAAACATGGGGAGTAATAGCGTTAACattat                                                                                                                        <  1:44489/250‑1 (MQ=255)                                                                                                                            cATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCATTCTCCTCATGATGGACTAGTTATAATAAATAAAGGAAACGAAGAAGTTGAAGGTACCTGGCCTAACAAATTGCAACCTGGAATATACAAAAACATGGGGAGTAATAGCGTTAACATTATTATTAATAATACCCGAAAAATTATcccc                                                                                            <  1:108583/251‑1 (MQ=255)                                                                                                                                                                                                                       gAACATTAATCATTCTCCTCATGATGGACTAGTTATAATAAATAAAGGAAACGAAGAAGTTGAAGGTACCTGGCCTAACAAATTGCAACCTGGAATATACAAAAACATGGGGAGTAATAGCGTTAACATTATTATTAATAATACCCGAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAAccg  <  1:395790/250‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              ATGGCCAAGTATTAAAATCAACGACTGAAATTAAAAATTATGATGGGTCCACGCGTGTCGGCGGTGAGGCGTAGCTTAATAAAGGTTGCTCTACCTATCAGCAGCTCTACAATGAATTCCACGCATTATATATAGATAAAGCAGGAATATGGAGCGCATTTGAGGGTGTATCTGCAACTAAAAACGTTCTGGCCTTTAGTCGTGGGGATTCTGTGAACATTAATCACTCTCCTCATGATGGACTAGTTATAATAAATAAAGGAAACGAAGAAGTTGAAGGTACCTGGCCTAACAAATTGCAACCTGGAATATACAAAAACATGGGGAGTAATAGCGTTAACATTATTATTAATAATACCCGAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCG  >  NC_000913/2788165‑2788628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑