Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,710,736	C→T	T106I (ACC→ATC)	nadB →	L‑aspartate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,710,736	0	C	T	100.0%	116.6 / NA	34	T106I (ACC→ATC)	nadB	L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/25);  total (9/25)

GCGCTACGCCTGGCTGACCAGCATCAGGTCATCGTTCTAAGTAAAGGCCCGGTAACGGAAGGTTCAACATTTTATGCCCAGGGCGGTATTGCCGCCGTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGCGACGGGTTGTTGGCGCGTGGGTATGG  >  NC_000913/2710489‑2710974                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               gcgcTACGCCTGGCTGACCAGCATCAGGTCATCGTTCTAAGTAAAGGCCCGGTAACGGAAGGTTCAACATTTTATGCCCAGGGCGGTATTGCCGCCGTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCca                                                                                                                                                                                                                                             <  2:241944/251‑1 (MQ=255)      aCGCCTGGCTGACCAGCATCAGGTCATCGTTCTAAGTAAAGGCCCGGTAACGGAAGGTTCAACATTTTATGCCCAGGGCGGTATTGCCGCCGTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACAt                                                                                                                                                                                                                                          <  1:85689/249‑1 (MQ=255)                   cAGCATCAGGTCATCGTTCTAAGTAAAGGCCCGGTAACGGAAGGTTCAACATTTTATGCCCAGGGCGGTATTGCCGCCGTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCga                                                                                                                                                                                                                           <  1:181537/251‑1 (MQ=255)                      cATCAGGTCATCGTTCTAAGTAAAGGCCCGGTAACGGAAGGTTCAACATTTTATGCCCAGGGCGGTATTGCCGCCGTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCgc                                                                                                                                                                                                                           >  1:221031/1‑247 (MQ=255)                                    tCTAAGTAAAGGCCCGGTAACGGAAGGTTCAACATTTTATGCCCAGGGCGGTATTGCCGCCGTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTg                                                                                                                                                                                                           >  1:157606/1‑250 (MQ=255)                                                    gTAACGGAAGGTTCAACATTTTATGCCCAGGGCGGTATTGCCGCCGTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGAc                                                                                                                                                                                           >  2:305683/1‑250 (MQ=255)                                                        cGGAAGGTTCAACATTTTATGCCCAGGGCGGTATTGCCGCCGTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGt                                                                                                                                                                                      <  2:86428/251‑1 (MQ=255)                                                            aGGTTCAACATTTTATGCCCAGGGCGGTATTGCCGCCGTGTTTGGTGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCTCGAGCCTGTGTGCAATGGCTAATCGACCAGGTGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCAcc                                                                                                                                                                                  <  1:209775/251‑1 (MQ=255)                                                                       tttAGGCCCAGGGCGGTATTGCCGCCGTGTTTGATGAAACTGACAGCATTGACGCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTAttct                                                                                                                                                                        <  2:160185/250‑1 (MQ=255)                                                                                            ccgccgTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGt                                                                                                                                                  <  1:70952/251‑1 (MQ=255)                                                                                             cgccgTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGt                                                                                                                                                  >  1:358926/1‑250 (MQ=255)                                                                                                cgTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTag                                                                                                                                                >  1:436364/1‑249 (MQ=255)                                                                                                   gtTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGaa                                                                                                                                            <  1:322887/250‑1 (MQ=255)                                                                                                                        tGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGc                                                                                                                       <  2:179705/250‑1 (MQ=255)                                                                                                                         gACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATATTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCaa                                                                                                                     <  1:194860/251‑1 (MQ=255)                                                                                                                               cATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCt                                                                                                               <  2:207777/251‑1 (MQ=255)                                                                                                                                  gtgGAAGACACATTTATTGCCGGTGCTGGTATTTGCGATCGCCATGCATTTGAAGTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGATCAGGGTGTGTTGTTTGATATCCACATTCATCCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTCTTCTTCATGCCGCCGACGCCGCTGGTAGAGAAGTAGAAACCACGCTGGTGAGCTAGGCTCTGaa                                                                                                            <  1:344836/251‑1 (MQ=255)                                                                                                                                        gACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCTAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATcc                                                                                                      <  1:399804/251‑1 (MQ=255)                                                                                                                                         acacATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATg                                                                                                                                                                                                                               <  1:79928/129‑1 (MQ=255)                                                                                                                                               tgattgCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATAtt                                                                                               >  1:7383/1‑251 (MQ=255)                                                                                                                                                gattgCCGTGGCTGGTATTTGCGATCGCCAGGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTGGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTc                                                                                              <  1:250893/251‑1 (MQ=255)                                                                                                                                                          gCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGa                                                                                    <  1:261361/251‑1 (MQ=255)                                                                                                                                                                     cGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAAcg                                                                         >  1:84351/1‑251 (MQ=255)                                                                                                                                                                            cATGCAGTGGAATTTGTCGCCAGCAATGCACGATTCTGTGTGCAATGGATAATCGACCAGGGGGTGTTGTGTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGGATTCTTCATGACGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTTGTGAGCAAGGCGCGGAACCATCCGAATATTCTCGTGCTGGAGCGCAGCAACGCGGTTGa                                                                  <  2:221031/251‑1 (MQ=255)                                                                                                                                                                             aTGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCAGCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGAt                                                                 <  2:27669/251‑1 (MQ=255)                                                                                                                                                                                    tGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGAtt                                                           <  2:436364/250‑1 (MQ=255)                                                                                                                                                                                       aTTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGttt                                                       <  2:61866/251‑1 (MQ=255)                                                                                                                                                                                                 aGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAAt                                             >  1:208459/1‑251 (MQ=255)                                                                                                                                                                                                                 gtgtgCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATGCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTAGTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTTACAAAATTGGCCGGCCGGGCAc                              <  2:201543/250‑1 (MQ=255)                                                                                                                                                                                                                  tgtgCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCAcg                             <  1:12492/250‑1 (MQ=255)                                                                                                                                                                                                                     gCAATGGCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGCGAc                         <  1:144503/251‑1 (MQ=255)                                                                                                                                                                                                                          ggCTAATCGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGCGACGGgtt                    <  1:202887/251‑1 (MQ=255)                                                                                                                                                                                                                                 cGACCAGGGGGTGTTGTTTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAACATTGGCCTGCCGGGCACGCGACGGGTTGTTGgcg             >  2:18729/1‑251 (MQ=255)                                                                                                                                                                                                                                             gttgttTGATATCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGCGACGGGTTGTTGGCGCGTGGGTATgg  <  2:102697/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               GCGCTACGCCTGGCTGACCAGCATCAGGTCATCGTTCTAAGTAAAGGCCCGGTAACGGAAGGTTCAACATTTTATGCCCAGGGCGGTATTGCCGCCGTGTTTGATGAAACTGACAGCATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATCCGAATATTCGCGTGCTGGAGCGCAGCAACGCGGTTGATCTGATTGTTTCTGACAAAATTGGCCTGCCGGGCACGCGACGGGTTGTTGGCGCGTGGGTATGG  >  NC_000913/2710489‑2710974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑