Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,510,609	C→T	P327L (CCG→CTG)	yfeO →	putative transport protein YfeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,510,609	0	C	T	100.0%	56.9 / NA	20	P327L (CCG→CTG)	yfeO	putative transport protein YfeO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/4);  total (16/4)

ATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGTCGTTGTACCCAAT‑ACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGT  >  NC_000913/2510377‑2510829                                                                                                                                                                                                                                         |                                                                                                                                                                                                                              atgatgCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGGATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTTAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCGTTCGCGGGGGGCGGATCTTTCTTGCAGGGTTTGTCGGCgg                                                                                                                                                                                                             >  1:235926/1‑250 (MQ=255) atgatgCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGGATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATACAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCCGGTCGTTGCTGTCGCCAGTGGCTTTCTCGGCGGGCGGCTCTTCCTGGTAGTGTTTGTCGGCgg                                                                                                                                                                                                             >  1:400912/1‑250 (MQ=255)    atgCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGCTTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGAAATTAAACTTGCCGCCCTGGGCGTTGCTGCCGCCAGGGGCTTTTGCGGGGGGCGAATCTTCCTGGCAGGGTTTGTTGGCggggg                                                                                                                                                                                                          >  1:385152/1‑247 (MQ=255)      gCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTTTTTGTCGGCTTGGCAt                                                                                                                                                                                                        <  1:332551/251‑1 (MQ=255)                                         ttGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCgg                                                                                                                                                                                                                <  1:416659/208‑1 (MQ=255)                                               gATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAAATTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGGTTGTCGGCGTTGCATTTGGGTTGATGCTGCATGAGCCCGTTCCCGCCGTAACAGCg                                                                                                                                                               >  2:113452/1‑251 (MQ=255)                                                             ttttggggggTTTTGGGGGGACCAGTTTCGTTTTTTAAAGGGGTGAAGGAGAGGCAGAAGGGGGGGGAAAACCGGGCTTTCAGCCCCAGCGTTTTTTTTTTGCGGGGGTTAATTAAACTTCCCCCCCTGGTCGTTGCGCCCGCCGGTGGCTTTCGGGGGGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGGGGTATTGGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGttt                                                                                                                                                  <  1:311533/245‑1 (MQ=255)                                                                       ttATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGGCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGGGTTGGTCGGCGTGGCATTAGGGGTGATGCTGCATGAGCACGTTCCCGCCGGACCAGCGGCCATAACCGGTTTTTTCGCTAtt                                                                                                                                       >  1:235202/1‑251 (MQ=255)                                                                                                tAAAGGGCTGGAAGAGATGCAGCAGCTGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTCCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTCGCAGTGGTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCCGCGGCAATAAACGTTTCTTGCGCTATTCTCGGCGACGTGCTGGTGGTAccac                                                                                                              >  2:319455/1‑251 (MQ=255)                                                                                                        tGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTAt                                                                                                                                        >  1:333099/1‑217 (MQ=255)                                                                                                                                 aaaTCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGGGGCTTTCGCGGGGGGCGAATCTTCCTGGCCGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGGAACACGCGATGGCTGGTTAAGTCTTTTTATggcggg                                                                               >  2:231667/1‑248 (MQ=255)                                                                                                                                    tCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAg                                                                                                                                                                                    <  1:421776/145‑1 (MQ=255)                                                                                                                                    tCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGGAATTCAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGGGGGCGAATCTTTCTGGCCGTGTTTGTCGGCGGGGCCTTAGGGGTGGTGCTGCATgtg                                                                                                                                                                                    >  2:421776/1‑143 (MQ=255)                                                                                                                                                                      gCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGTCGTTGTACCCAAT‑ACCACATTGCTACCGCTGCTCTg                                       >  1:428258/1‑251 (MQ=255)                                                                                                                                                                             ttAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGt                                                                                                  >  2:141583/1‑186 (MQ=255)                                                                                                                                                                                 aCTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGAGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGTCGTTGTAACCACT‑ACCACATTGCTACCGCTGCTCTTGATCGTCATGc                            >  2:408977/1‑251 (MQ=255)                                                                                                                                                                                            tGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCCTTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGAGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGACGTCGGTGTCCCCAAT‑TCCACATTGCTTACGCTGCTCTGTTTCGCAATGATTCCCGCATgg                 >  1:290305/1‑251 (MQ=255)                                                                                                                                                                                                 gTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGACTCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGTCGTTGGACCCAATCACCA‑ATTTCTACCGCTGTTCTGTATCGTCCTTGTTCCGGCAAGGCTGtt            >  2:417298/1‑251 (MQ=255)                                                                                                                                                                                                         cgccAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGTCGTTGTAACCAAT‑ACCCCCATGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCCTGGCTGttatttgc      >  1:344607/1‑246 (MQ=255)                                                                                                                                                                                                           ccAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGTCGTTGTACCCAAT‑ACCACATTGCTACCGCTCCTCTGTATCGTCATGCTTCCGGCCTGGCTGttttttgctggg  >  2:284239/1‑241 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                              ATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGTCGTTGTACCCAAT‑ACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGT  >  NC_000913/2510377‑2510829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑