Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,485,434	C→T	A354V (GCT→GTT)	evgS →	sensor histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,485,434	0	C	T	100.0%	33.4 / NA	11	A354V (GCT→GTT)	evgS	sensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/3);  total (8/3)

CTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTT  >  NC_000913/2485219‑2485660                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   cTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGTTGGAACACAGCTTAGCCCCGGAGGATGGGAtata                                                                                                                                                                                                  >  1:42236/1‑250 (MQ=255)               cATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCACAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGTTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTAtt                                                                                                                                                                                   <  2:341549/251‑1 (MQ=255)                aTTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATAACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGTTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTAttt                                                                                                                                                                                  >  1:90187/1‑251 (MQ=255)                  tAGAACTCACTGCACATGAAAAACAGTGGATTAAGCAGCATCCCAATTCAAAGGTGCTGGAAAATCCTTACTCGACCCCCTATTCTATGCCGGATGAAAATGGCTCGGTTCGGGGAGATATGGGGTACATTCTTAATATTATTACCTTGAAAACAGGTTTAAATTTTTCTCCGATAACCGTTTCACACAATATCCATGTTGGACAACCGCTTAGCCCCGTAGTGTGGGATATAATACCAGGCGCTATTtat                                                                                                                                                                                >  2:422595/1‑251 (MQ=255)                                       aaCAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAACTGGATCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCACACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGTTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAaataat                                                                                                                                                           >  2:203772/1‑251 (MQ=255)                                                                                                                        gCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGTTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTaaa                                                                           >  1:280606/1‑250 (MQ=255)                                                                                                                                                        aaTATTCTTACCTTGCAACCAGGTTTCAATTTTTCTCCGATCACCGTTTCACACAATATCCATGTTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATACTGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCAtattatt                                           >  2:278847/1‑250 (MQ=255)                                                                                                                                                            ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGTTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAgc                                                                                                   >  1:44921/1‑190 (MQ=255)                                                                                                                                                            ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGTTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAgc                                                                                                   <  2:44921/190‑1 (MQ=255)                                                                                                                                                                                            ccGATCACCGTTTCACACAATATCCATGTTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCAATTAAAAGAGATGTATcc         <  2:93488/248‑1 (MQ=255)                                                                                                                                                                                                tCACCGTTTCACACAATATCCATGTTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGtt  >  1:230446/1‑251 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   CTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTT  >  NC_000913/2485219‑2485660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑