Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,473,392	G→A	intergenic (‑146/‑212)	yfdO ← / → yfdP	protein YdfO/uncharacterized protein YfdP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,473,392	0	G	A	100.0%	63.5 / NA	19	intergenic (‑146/‑212)	yfdO/yfdP	protein YdfO/uncharacterized protein YfdP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/9);  total (10/9)

GGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTT‑ACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGC  >  NC_000913/2473164‑2473618                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    ggACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGc                                                                                                                                                                                                               >  1:245346/1‑251 (MQ=255)         caccTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCt                                                                                                                                                                                                       >  2:220203/1‑251 (MQ=255)               gCCGTCACATTCGGCGGCCTGCTCGTACAGGTCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAA‑TCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGCCGGATCCCCGCCAGATTCTGACGGGt                                                                                                                                                                                                <  2:383890/251‑1 (MQ=255)                    cacaTTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTGCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGAGGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACTGATCCCCGCCAGATTCTGACGGGTGaaa                                                                                                                                                                                            <  2:222237/251‑1 (MQ=255)                               gcCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGAttttt                                                                                                                                                                                 >  1:228466/1‑251 (MQ=255)                                   gCTCGTACAGGGCGATGATTTTTTTCCAAAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTgac                                                                                                                                                                             >  1:333962/1‑249 (MQ=255)                                     ccGTACAGGGCGAGTATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGACAg                                                                                                                                                                           <  2:333962/250‑1 (MQ=255)                                                             cagagaccctgagcgcAGGTAAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGCTTTCGACGCATCAAATTTTGACg                                                                                                                                                   >  2:426867/13‑251 (MQ=255)                                                                           gcAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGAt                                                                                                                                     >  1:126098/1‑251 (MQ=255)                                                                               gTCAACTCATCCTGCGTTCCCCACTGACGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTACGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGACAGATTCTGACGGGTGAAAACCCGCTTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGt                                                                                                                                 >  2:417195/1‑251 (MQ=255)                                                                                                              ttttAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGCAGGTTTTTTATCTGACGGATCCTGTTTTAATTTTTACTGACGGGTCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATGTTGACGGGTCAGATTTTGATGCGTCAGATTTTGAGGGGTCAGAGTCTGACAGTTg                                                                                                  >  1:279657/1‑251 (MQ=255)                                                                                                                               aCCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCt                                                                                 <  2:126098/251‑1 (MQ=255)                                                                                                                                                           gcTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCTTGAAGCTTCGCAACGTTAAGCTGATaaa                                                      <  2:8303/249‑1 (MQ=255)                                                                                                                                                              gTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGTATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATTAAAGTTTGACGTGTCAGATTTTGATGCGGCAGATTTTTACGGGGCATAGTCTGACAGTTGAGAAAATGCCGCTTCCTGAAGCTTCTCAACGTTAATCGGATAAACAt                                                   <  2:427697/250‑1 (MQ=255)                                                                                                                                                                                 cGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTACCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTGTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTGTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTatga                               <  1:290505/251‑1 (MQ=255)                                                                                                                                                                                   gTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTatgatg                             <  1:131107/251‑1 (MQ=255)                                                                                                                                                                                                cGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCaaa                 >  1:184030/1‑250 (MQ=255)                                                                                                                                                                                                    cGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACTCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGc            <  1:84806/251‑1 (MQ=255)                                                                                                                                                                                                                ttATCTGACGGATCATGTTTTAATTTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGGAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGGGGAGCAGCAAAATGGCTAGCGAACGc  >  1:424786/1‑249 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    GGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTT‑ACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGC  >  NC_000913/2473164‑2473618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑