Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,471,806	G→A	intergenic (‑289/+306)	yfdK ← / ← yfdM	putative tail fiber assembly protein YfdK/putative methyltransferase YfdM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,471,806	0	G	A	100.0%	48.2 / NA	16	intergenic (‑289/+306)	yfdK/yfdM	putative tail fiber assembly protein YfdK/putative methyltransferase YfdM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/7);  total (9/7)

GAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTCCTGGTTATAACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAAGCAGCTGGTCATGCCGCCCCTTCTCCAGGCT  >  NC_000913/2471559‑2472017                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                    gAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTCCTGGTTATAACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCGTAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGaa                                                                                                                                                                                                                  <  1:179723/251‑1 (MQ=255)  aaaaaTGCAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATATCACCGGGTCCTGGTTATAACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGACCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAAc                                                                                                                                                                                                                 >  2:179872/1‑251 (MQ=255)                 aTACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTCCTGGTTATAACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCAt                                                                                                                                                                                                  <  1:120557/251‑1 (MQ=255)                    ccGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTCCTGGTTATAACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCATaaa                                                                                                                                                                                               <  1:156605/251‑1 (MQ=255)                          aCGCCTGCTCTGTAAATAAATTGCACCGGGTCCTGGTTATAACCGGCAAAAGCTATATAGCCAACATTTGCACTGCCGGGAGTTTAATCCGGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCACCATTGAATAAATAAACCGGATGTCAATTTTTGATATCCAAGAACTTAAGCATAAAGCATAATACTAcc                                                                                                                                                                                         <  2:372679/251‑1 (MQ=255)                                                                                  ataGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTccc                                                                                                                                  >  1:177995/1‑250 (MQ=255)                                                                                                                        tCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTc                                                                                                                                                   <  2:343488/195‑1 (MQ=255)                                                                                                                             aaTACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATc                                                                                                                                                                            <  1:198416/165‑1 (MQ=255)                                                                                                                             aaTACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCATGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATc                                                                                                                                                                            >  2:198416/1‑165 (MQ=255)                                                                                                                                   agCAGACATCTATTCGGAAATGCAATCGGAAACTAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTACACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCATACAACTACCCATATAAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCCAGGTTTTCGCGAGCCTTTTGCACCGTGCCGTCCAATTTGatctc                                                                                >  2:247419/2‑248 (MQ=255)                                                                                                                                       gACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGcc                                                                             >  1:170378/1‑250 (MQ=255)                                                                                                                                          aTCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCATAAAACTACCCATATAAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAAc                                                                         >  2:89346/1‑251 (MQ=255)                                                                                                                                                                 aaaTAGGATACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCACCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCACTTTGATATCGCCAAACGGATTCTTGCGGCTTAAcagc                                                    >  1:423310/1‑249 (MQ=255)                                                                                                                                                                          aCTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGa                                           >  1:21692/1‑249 (MQ=255)                                                                                                                                                                          aCTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAAGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAg                                         <  1:326542/251‑1 (MQ=255)                                                                                                                                                                                                                 cATTGAATAATTAAACCGGATGGCAATTTTTGCTATCCAAGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAAGCAGCTGGTCATGCCGCCCCTTCTCCAGGCt  >  1:96150/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                    GAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTCCTGGTTATAACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAAGCAGCTGGTCATGCCGCCCCTTCTCCAGGCT  >  NC_000913/2471559‑2472017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑