Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,443,447	G→A	A562T (GCG→ACG)	mnmC →	fused tRNA 5‑aminomethyl‑2‑thiouridylate methyltransferase and tRNA 5‑aminomethyl‑2‑thiouridylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,443,447	0	G	A	100.0%	118.5 / NA	34	A562T (GCG→ACG)	mnmC	fused tRNA 5‑aminomethyl‑2‑thiouridylate methyltransferase and tRNA 5‑aminomethyl‑2‑thiouridylate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/16);  total (18/16)

GGTCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGGCGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCGATGGAT  >  NC_000913/2443209‑2443689                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   ggTCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGgtg                                                                                                                                                                                                                                        >  1:42620/1‑251 (MQ=255)                gACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCAccc                                                                                                                                                                                                                         <  1:30038/251‑1 (MQ=255)                                     gCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAg                                                                                                                                                                                                    <  2:395965/251‑1 (MQ=255)                                                   tgtgctATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGAATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGAt                                                                                                                                                                                      >  1:255545/1‑251 (MQ=255)                                                                 tagctcACGCCACAAAATCCGGCGAATCAACATCATTGTATGGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGAGTATGAGGCAacac                                                                                                                                                                         <  2:353883/247‑1 (MQ=255)                                                                       aCGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGa                                                                                                                                                                  <  1:21281/251‑1 (MQ=255)                                                                            ccaacaTCCGGAGAATCAACATCATTGTATTGGTGCAAGTTATCATCGCGGCAGCGAAGAAACGGCGTACAGTGAGGACGATCAGCAGCAGCATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCCGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGCTCATCTCCCAATGGTAGGCAATGTTCCCGATTCTGAGGCACCACTCGTGGAatctg                                                                                                                                                             >  2:243643/6‑248 (MQ=255)                                                                               aaaTCCGGCGAATCACCATCATTGTATTGGTGCCCGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGcgt                                                                                                                                                          >  2:293774/1‑251 (MQ=255)                                                                               aaaTCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGcgt                                                                                                                                                          <  1:376675/251‑1 (MQ=255)                                                                                aaTCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGcgtc                                                                                                                                                         >  1:180793/1‑251 (MQ=255)                                                                                                     tGTATTGATGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATg                                                                                                                                     >  1:126037/1‑250 (MQ=255)                                                                                                      gTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGa                                                                                                                                    <  1:159272/250‑1 (MQ=255)                                                                                                       tATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAgg                                                                                                                                  >  1:124564/1‑251 (MQ=255)                                                                                                                  gTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAgcgcg                                                                                                                       <  2:410449/251‑1 (MQ=255)                                                                                                                      tcatcGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCCGCAGAATCGCCAGCGGTTGATTGATTGTTTACCGCCGGCCCAGTGGGCAACAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGAAAGGGCGCCgg                                                                                                                   >  1:258910/1‑251 (MQ=255)                                                                                                                          cgcgGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGttt                                                                                                                <  1:30078/250‑1 (MQ=255)                                                                                                                                aGCGAAGATACGAAGTACAGTGAGGACGATCAGCAGCAGACTCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTggcg                                                                                                          >  2:78403/1‑247 (MQ=255)                                                                                                                                   gAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGGTTTTgtcggtct                                                                                                      >  2:26416/1‑244 (MQ=255)                                                                                                                                          cGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGa                                                                                                            <  2:159608/238‑1 (MQ=255)                                                                                                                                          cGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGGTTTTGa                                                                                                            >  1:159608/1‑238 (MQ=255)                                                                                                                                                          cGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCGTTAGGtt                                                                               <  2:65364/251‑1 (MQ=255)                                                                                                                                                                     agAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGTTCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTtg                                                                    >  2:132269/1‑251 (MQ=255)                                                                                                                                                                               gCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTAGGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGccc                                                          <  1:377697/251‑1 (MQ=255)                                                                                                                                                                                      attgattgTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGGTTTTGACGATCTCTTTTTGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCtgtg                                                   >  1:308722/1‑251 (MQ=255)                                                                                                                                                                                             gTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCga                                             <  1:102358/250‑1 (MQ=255)                                                                                                                                                                                              tttCCCGCAGGCACAGTGAGCAAAAGAGGCTGATGTCAGTGATAAAGAGAAGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGCGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACCATCTCTTTATGTTTGTGGCTTTAGGGTTTCGCGGTTTGTGTTTTGCCCCGCcgtgtggcgcg                                            >  2:290878/1‑245 (MQ=255)                                                                                                                                                                                                 cccGCAGGCACAGTGGGCCAGCGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGCTGCGCCACCCGCGACCATCTGCCAATGGTCGGCAATGTGCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGATTTTGACGGTCCCTTTGTGTTTGGGGTTTTAGGTTCTTGCGGTTTGTGTTCTTCACCTCTGTGTGCCgagaatc                                        >  2:166616/1‑248 (MQ=255)                                                                                                                                                                                                 cccGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTc                                        <  2:366180/251‑1 (MQ=255)                                                                                                                                                                                                               gggCAAAAGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGAt                           <  1:233417/250‑1 (MQ=255)                                                                                                                                                                                                                   aaaaGAGGTTGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCtgtg                                                   <  1:432149/222‑1 (MQ=255)                                                                                                                                                                                                                            tGATGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCg              >  1:6823/1‑250 (MQ=255)                                                                                                                                                                                                                              aTGTCAGTGATAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGAtt           <  1:26416/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTTTGCCCCGCTGTGTGCCGAGATTCTGGCGGGGCCGATGAGCGACGGAACGATTCCgctggat  >  1:211268/1‑251 (MQ=255)                                                                                                                                                                                                                                       aTAAAGAGACGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCgatggat  >  1:394035/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   GGTCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAAGAGGCGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCGATGGAT  >  NC_000913/2443209‑2443689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑