Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,392,233	C→G	G270A (GGT→GCT)	nuoM ←	NADH:quinone oxidoreductase subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,392,233	0	C	G	100.0%	165.4 / NA	48	G270A (GGT→GCT)	nuoM	NADH:quinone oxidoreductase subunit M
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (19/29);  total (19/29)

CGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGG  >  NC_000913/2391989‑2392475                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      cggcAAACCGTGCGCAATCATCTGGATGACCGCGCCCTGGTAGGCCAAGTGGCGGCCTGTGTAGATAGCAATCAGCAAGAAGACCATGTGGGAAACCGAGGTGTAGGCGATCAGACGGTAGATATGCGTCTGGGCGAAGGCTATCCACGCACCGTAGAAGATGCCGATAACTCACAGCCACAGAGCAATTGGCGCGAACACTGCCGACGAGTTCGGGAACAGCGGCAGGGAGAATCGCAGCAAAGCGTAAg                                                                                                                                                                                                                                                 <  2:214311/248‑1 (MQ=255)        ccGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGCTGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCCGCAAAGCGTAAGCGGCAGt                                                                                                                                                                                                                                          >  2:28846/1‑251 (MQ=255)         cGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAACCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGAGCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGtt                                                                                                                                                                                                                                         <  2:408414/251‑1 (MQ=255)            gcgcAATCATCTGGATGACCGCGCCCTGGTAGGCCACCTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGAAGCGAGAAACGCAGCAAAGCGTAAGCGTCTGtttt                                                                                                                                                                                                                                       <  2:32746/250‑1 (MQ=255)                   catcTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTATAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAg                                                                                                                                                                                                                                <  1:325237/250‑1 (MQ=255)                       tGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATccc                                                                                                                                                                                                                           >  1:307155/1‑251 (MQ=255)                        ggATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGAGCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATc                                                                                                                                                                                                                             <  2:238191/248‑1 (MQ=255)                               cgcgCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCATCAAGATCCCCGCGAGGt                                                                                                                                                                                                                   <  1:345680/251‑1 (MQ=255)                               cgcgCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATAAGCACGCAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCAGATCACACACAGCCACATAGCAATTGGCGCGAACTCTGCAGACGCGATCGGGAACAGCGGCAGGGAGAAACGCAGCCAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGt                                                                                                                                                                                                                   >  1:185588/1‑251 (MQ=255)                                   cccTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAAGTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAAc                                                                                                                                                                                                               <  2:229023/251‑1 (MQ=255)                                               aaCTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGAGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGCGGTCAACGGAACcggcggg                                                                                                                                                                                                   >  1:160818/1‑250 (MQ=255)                                                                            cACGCAGCCCATGTGGGAAACCGAGGCGTAGGAGATCACCCGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGCCACGTTCGAGACCAGAGGCAGGGAGAAACGCAGCAAAGCGGAAGCGGCAGGTTTCAGCCAGATCCCCGCGAGGTCAACGGAACCGGCGGGCGGGGCCGGGG‑GGTgctcccacagccgcc                                                                                                                                                                     >  2:241812/1‑238 (MQ=255)                                                                               gAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑GGGGCGGATCC‑gcgagcccgc                                                                                                                                                                 >  2:376135/1‑242 (MQ=255)                                                                                aaGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGGGCCTggggggggcgcacggggggagggcg                                                                                                                                                                  >  2:416566/1‑229 (MQ=255)                                                                                aaGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTgc                                                                                                                                                                                  <  1:416566/234‑1 (MQ=255)                                                                                aaGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGGCGGGGCCTGGG‑GGGGCGCATcccgcggacagcc                                                                                                                                                                 >  2:437148/1‑240 (MQ=255)                                                                                     cATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCa                                                                                                                                                           <  2:353310/251‑1 (MQ=255)                                                                                               aCCGAGGTGTAGGCGATCAGACGGTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACTCCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAaccac                                                                                                                                                 <  1:392325/251‑1 (MQ=255)                                                                                                  gAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCg‑g                                                                                                                                             <  1:376135/251‑1 (MQ=255)                                                                                                    ggTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGTCATGCAGCGGATCCACCG‑GCa                                                                                                                                           <  2:292685/251‑1 (MQ=255)                                                                                                       gtAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCAttt                                                                                                                                        <  2:307155/251‑1 (MQ=255)                                                                                                                     gTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCg                                                                                                                          <  1:25845/251‑1 (MQ=255)                                                                                                                            atCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATACCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGGGAG‑GCGCATCC‑GGCAGCCAGCCCTGCAGCGGAACCACCG‑GCCTTTTGACTGACAAGGCggggaaa                                                                                                                    >  1:3669/1‑244 (MQ=255)                                                                                                                              cGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATgaagaa                                                                                                                  <  1:152892/250‑1 (MQ=255)                                                                                                                              cGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATgaagaa                                                                                                                  <  1:131257/250‑1 (MQ=255)                                                                                                                               ggTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGGCGGGGCACGGG‑GGGGCGCAGCC‑GGCACCCCGCCAAGCAGCGGAAACACCG‑GCATTTTGACTGCggagagggtggagaagc                                                                                                                >  2:259726/1‑235 (MQ=255)                                                                                                                                      gCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAATCCCAGCAt                                                                                                         <  1:91331/251‑1 (MQ=255)                                                                                                                                           ggCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACa                                                                                                    <  2:252523/251‑1 (MQ=255)                                                                                                                                                     gCACCGTAGAAGATGCCGGTAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGGGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGTATGCGAAGGCGATGAAGAAGCCCAGCATTTACAGGGATTCcac                                                                                          >  1:329094/1‑251 (MQ=255)                                                                                                                                                          gTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑GGTGCGCCTCC‑GGCAGCCAGCCATGCCACGGAACCACCG‑CCATTTTGCCCGAGAAGGCGATGAAGAAACCAAGCATTAACAGGAATTACCcaccac                                                                                     >  1:232945/1‑251 (MQ=255)                                                                                                                                                              aagaTTCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGa                                                                                 <  2:126166/251‑1 (MQ=255)                                                                                                                                                                 aTGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACAt                                                                              <  1:28846/251‑1 (MQ=255)                                                                                                                                                                     cGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGGCGGAGCCTGGG‑GGTGCGCATCC‑GGCAGACAGCCCTGCAGCGGGACCCCCG‑GCCTTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTccccccccacggacattggg                                                                          >  2:428444/1‑233 (MQ=255)                                                                                                                                                                      gATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGGGCCTGGG‑GGTGCGCATCC‑CGCCGCCCGCCCTTCAACGGAACCACCG‑GCCTTTTGACCACTGAGGCGATGAAGAAGCCCCCCATTTACCGGGATTCCACACCCCTCGACATTTGCg                                                                         >  1:420978/1‑251 (MQ=255)                                                                                                                                                                       aTAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGGGCCTGGG‑AGGGCGCATCC‑GGCAGCCAGCCCTGCAGCGGAACCCCCG‑GCATTTTGTCTTCGAAGGCGAAGAAGAAGCCCAGCATTAAAAGGTAtttcccacccactggcaatggcgg                                                                        >  1:329961/1‑229 (MQ=255)                                                                                                                                                                             cccAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGGGCCTGGG‑GGAGAGCCTCCCGGC‑GCCCGCCATGCCGCGGAACCAACGCGC‑TTTTTACTGCGAAGGCGATAAAGAAACCCAGAGTTAACAGGGATTTCACACCCCTGGACATTgggggattc                                                                    >  2:371655/1‑242 (MQ=255)                                                                                                                                                                             cccAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTcag                                                                  <  2:329094/251‑1 (MQ=255)                                                                                                                                                                              ccAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGGGCGCCTCC‑CGCCGCCAGCCATGGAGCGGGAACACCC‑GGATTTTGACTGCGGAGGGGCTGCAGAAACCCCGCCGTAAAAGGGATTCCACACCCCCGGACATTGGcctcatcagc                                                                 >  2:153300/1‑242 (MQ=255)                                                                                                                                                                                 gCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCagcagc                                                              <  1:437148/251‑1 (MQ=255)                                                                                                                                                                                   cacaTAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACTCCACTGGACATTGGCGTATTCATCAGctc                                                            <  1:334532/251‑1 (MQ=255)                                                                                                                                                                                        aGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCAt                                                       <  1:428444/251‑1 (MQ=255)                                                                                                                                                                                        aGCAATTGGCGCGAACCCGGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCAt                                                       <  2:372258/251‑1 (MQ=255)                                                                                                                                                                                           aaTTGGCGCGAACTCGGCCGACGCGGTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCTCAGCAGTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGt                                                    <  2:420978/251‑1 (MQ=255)                                                                                                                                                                                                            ccGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATTGTTGAAGGTCCAGACGCCg                                   <  1:343343/251‑1 (MQ=255)                                                                                                                                                                                                              gACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGt                                 <  1:259726/251‑1 (MQ=255)                                                                                                                                                                                                                   gtgcGGGAACAGCGGCCGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCTAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGGTCGCATCC‑GGCAGGCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTTGCGTATTCAGCATCTCTTCATAGTTGAAGGCCCTGACGCCGGTCGCAt                            <  2:89076/248‑1 (MQ=255)                                                                                                                                                                                                                                   aGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGACCGGG‑GGGGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCC‑GCCTTTTTACTGCGAAGGCGATGAAGAAGCCCAGCAATAACAGGGATTCCACACCACCGGACATTGGCGGATTCAGCAGCCCTTTCTAGTTGAAGGGACAAGCGCCCGTCGCCTTGTAGGGAACcaaaa             >  2:241789/1‑250 (MQ=255)                                                                                                                                                                                                                                               agcaAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGGGGCTGGG‑AGGGCGCCTCC‑GGCAGACCGCCATGCAGCGGAAACACCG‑GCACTTTTACTGCGGAGGGGGTGAAGAAACCCAGCATTAACAGGGATTTCACAACCCTGGGCATTGGCGCATTCAGCAACTCTTTATAGTTGAAGGGCCAAACGCCGGTCCCATTGTAGTGAACAAAAAcaaccgcccgg  >  2:355717/1‑239 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGG‑AGTGCGCATCC‑GGCAGCCAGCCATGCAGCGGAACCACCG‑GCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGG  >  NC_000913/2391989‑2392475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑