Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,338,564	A→G	V286A (GTG→GCG)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,338,564	0	A	G	98.4%	206.1 / NA	62	V286A (GTG→GCG)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (26/35);  minor base T (0/1);  total (26/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTT  >  NC_000913/2338329‑2338804                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 acgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTa                                                                                                                                                                                                                                   <  2:82542/251‑1 (MQ=255)   gggTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTAcc                                                                                                                                                                                                                                 <  1:376539/251‑1 (MQ=255)     gTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAg                                                                                                                                                                                                                               <  2:248437/251‑1 (MQ=255)                   gcggTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAAtcttct                                                                                                                                                                                                                 <  1:293414/251‑1 (MQ=255)                    cggTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTtctc                                                                                                                                                                                                                <  1:172722/251‑1 (MQ=255)                      gTGACGAACCAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCg                                                                                                                                                                                                               >  2:94501/1‑250 (MQ=255)                        gACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATc                                                                                                                                                                                                            <  1:97322/251‑1 (MQ=255)                                   gccgcGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTGTGTCAGACTCGGCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCGCGATCAGGCGCGCttt                                                                                                                                                                                                 <  1:308044/251‑1 (MQ=255)                                       cgATGATGTCTTTCAGGGTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGttt                                                                                                                                                                                             >  2:286761/1‑251 (MQ=255)                                        gatgatGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGttt                                                                                                                                                                                             >  1:20610/1‑250 (MQ=255)                                          tgatgTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCCATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTAcc                                                                                                                                                                                          >  1:115843/1‑251 (MQ=255)                                              gTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCTCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTGCCGCGCGTTTTTCTTTTATCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGAt                                                                                                                                                                                      <  1:262469/251‑1 (MQ=255)                                                  ttCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATa                                                                                                                                                                                     <  1:332221/248‑1 (MQ=255)                                                  ttCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACGCGTCACGCAGCGCGCTGATGCCTTCCGCTCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACg                                                                                                                                                                                   <  2:412766/250‑1 (MQ=255)                                                    cAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGTCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTTCCTGATACGGaa                                                                                                                                                                                <  2:148697/251‑1 (MQ=255)                                                       gTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAAttt                                                                                                                                                                             <  1:375157/251‑1 (MQ=255)                                                            tGATCTTCGGCTGAACATGGTGCAATGCCACCATGATGATACCGAAAGAAACCGGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTAACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACAAGTTCAGACATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATAAGGAATTTCTTgg                                                                                                                                                                        >  1:101490/1‑251 (MQ=255)                                                             gATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTgg                                                                                                                                                                        >  2:118983/1‑250 (MQ=255)                                                             gATCTTCGGCCGACCATGGTGCAAGGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGATGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTGCCGCGCGTTGTTCTTTTTCCAGGTCCGCAAGCTTCTCGATCAGTCGCGCGTTGTTTACCTGATGCGGAATTTCGTgg                                                                                                                                                                        <  2:394510/250‑1 (MQ=255)                                                                  tCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGAc                                                                                                                                                                      <  2:2672/247‑1 (MQ=255)                                                                   cagcttaCCATGGTGCAAGGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGAGGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTGCGCGATCAGTCGCGCTTTGTTTACCTGATACGGAATTTCGTTGACGATa                                                                                                                                                                  <  2:25064/244‑1 (MQ=255)                                                                   cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAAATTCACCGATCGCAGCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCTCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATa                                                                                                                                                                  <  1:334605/250‑1 (MQ=255)                                                                        gACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGTGCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGGTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACTAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGtt                                                                                                                                                            <  1:410506/251‑1 (MQ=255)                                                                           cATGGTGCAATGCCACCATGGTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCa                                                                                                                                                         <  2:115843/251‑1 (MQ=255)                                                                                  cAATGCCACCATGTTGATACCGACAGAAACCTG‑AACTGGGTCTGGGAGTAGAGGATGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGACTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGCTCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGCCGATAATGGTTTCACGACCGGt                                                                                                                                                 >  2:349560/1‑251 (MQ=255)                                                                                      gCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGtttt                                                                                                                                              >  1:207541/1‑251 (MQ=255)                                                                                        caccaTGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTgg                                                                                                                                            <  2:55621/251‑1 (MQ=255)                                                                                         accaTGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGc                                                                                                                                           >  2:332581/1‑251 (MQ=255)                                                                                                      cGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACt                                                                                                                              <  2:83663/251‑1 (MQ=255)                                                                                                          agaaaCCAGCAACTGGGAATGGGAGTAGAGGTTGTTGAGCCCAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATCCCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGCTGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCAAGACCGGTTTTGGCGTCAACTTCCACTTCTg                                                                                                                          >  2:36088/1‑251 (MQ=255)                                                                                                                  gCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCTCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTCCCTGATGCGGAATTTCGTGGACGATAATGGTTTCACGCCCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAgcg                                                                                                                  <  1:280659/251‑1 (MQ=255)                                                                                                                  gCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCGCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCATTTCTGCGCGAg                                                                                                                    <  1:70906/249‑1 (MQ=255)                                                                                                                        gggTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGat                                                                                                             <  1:238601/250‑1 (MQ=255)                                                                                                                             tGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTc                                                                                                                                                                            >  2:367273/1‑182 (MQ=255)                                                                                                                             tGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTc                                                                                                                                                                            <  1:367273/182‑1 (MQ=255)                                                                                                                                 aGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTGTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTg                                                                                                   <  2:241575/251‑1 (MQ=255)                                                                                                                                     gagGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCcgc                                                                                               >  1:57559/1‑251 (MQ=255)                                                                                                                                       ggTTGTTGACCACAACTCAACAGCCCGAATCGCGTTTCAATTCAATCACGCTGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGACTTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCcgcg                                                                                              >  2:286894/1‑250 (MQ=255)                                                                                                                                           gttgAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACcg                                                                                          <  1:253603/250‑1 (MQ=255)                                                                                                                                             tgAGCCCAACTTCACCGACCGCATCGCGTTTCACATCAATCACGATGCGCATACCGTCTTTGTCAGACTCGGCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTGCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTGTTGGCGTCAACTTCCACTTTTGCGCGACCGCGGATATACACCTTGCCGCGACcggt                                                                                        <  2:365450/250‑1 (MQ=255)                                                                                                                                                              accgCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTAACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGAGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAAt                                                                      >  2:263589/1‑251 (MQ=255)                                                                                                                                                               ccgCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACg                                                                                                                                                       >  1:153211/1‑169 (MQ=255)                                                                                                                                                               ccgCATCGCGTTTCACTGCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTGTCACg                                                                                                                                                       <  2:153211/169‑1 (MQ=255)                                                                                                                                                                   aTCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATAc                                                                    <  1:52193/248‑1 (MQ=255)                                                                                                                                                                   aTCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATAc                                                                    >  2:52193/1‑248 (MQ=255)                                                                                                                                                                            cACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCCCGCAGAGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAGTTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCGTCTTCAATACCGCGACGACCGt                                                        >  2:312513/1‑251 (MQ=255)                                                                                                                                                                                     cACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGAtt                                                <  1:415713/250‑1 (MQ=255)                                                                                                                                                                                        gATGCGCATACCGTTTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTgccg                                            <  2:438445/251‑1 (MQ=255)                                                                                                                                                                                             gcATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTc                                       >  1:305425/1‑251 (MQ=255)                                                                                                                                                                                                tACCGTCTTTGTCAGACTCGTCACGCCGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTCCCTGATACGGAATTTCGTGGACGATAATGGTTTCACGCCCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAAt                                                                      >  2:197771/1‑217 (MQ=255)                                                                                                                                                                                                tACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAAt                                                                      <  1:197771/217‑1 (MQ=255)                                                                                                                                                                                                       tttGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCg                             >  2:338983/1‑251 (MQ=255)                                                                                                                                                                                                        ttGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTGCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCg                             <  1:263589/250‑1 (MQ=255)                                                                                                                                                                                                         ttgcAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTTCCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCGGATACGGAATTTCGTGTACGATAATGGTTTCACGACCGGTTTTTGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCgt                            <  2:6165/247‑2 (MQ=255)                                                                                                                                                                                                          gTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGcc                          <  1:118983/251‑1 (MQ=255)                                                                                                                                                                                                           tCAGACTCGTCACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCCGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACAATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGAAAGCTTCTTCAATACCGCGACGACCGATAATGATTGCCGCCGTCGGGAAGTCCGGccc                         >  1:39171/1‑251 (MQ=255)                                                                                                                                                                                                                     cACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATgtgt               >  1:216344/1‑251 (MQ=255)                                                                                                                                                                                                                     cACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATgtgt               >  1:217248/1‑251 (MQ=255)                                                                                                                                                                                                                     cACGCAGCGCGCTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATgtgt               >  1:197908/1‑251 (MQ=255)                                                                                                                                                                                                                          aGCGCGATGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCAt          >  2:290594/1‑251 (MQ=255)                                                                                                                                                                                                                             gcgcTGATGCCTTCCGCGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGCTTTCCATc         >  2:330950/1‑249 (MQ=255)                                                                                                                                                                                                                                  gATGCCTTCCGCGCGGTTTTCTTTTACCAGGTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGGTTTGGCGGCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCTGGATGTGTTCCATCAGCCCtt  <  2:281717/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 ACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTTTCTTTTACCAGTTCCGCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTT  >  NC_000913/2338329‑2338804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑