| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,237,105 | G→T | P217T (CCG→ACG) | mglC ← | D‑galactose/methyl‑galactoside ABC transporter membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,237,105 | 0 | G | T | 98.0% | 137.6 / NA | 49 | P217T (CCG→ACG) | mglC | D‑galactose/methyl‑galactoside ABC transporter membrane subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (30/18); total (30/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GTAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGA > NC_000913/2236864‑2237350 | gTAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCCGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTCCCGcc > 2:235086/1‑251 (MQ=255) tAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGcc > 1:58734/1‑250 (MQ=255) aCCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGCTACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGATCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAAt > 2:77462/1‑251 (MQ=255) ggTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGCACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCt > 1:14849/1‑251 (MQ=255) gacaccacGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCtt > 2:404721/3‑251 (MQ=255) accacGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTAcc > 1:38651/1‑251 (MQ=255) accacGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTAcc > 1:54638/1‑251 (MQ=255) accacGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATACCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTAcc > 2:11550/1‑251 (MQ=255) cgccaacccccccccccACCCCGCCGCGGAACGATCCCCCGCCTCCGACGCACGCGGCAATA‑GCATCCAGCGCATACATAAAGCCGAGGTTTTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACTCGCCAGAAAACGCGTAAATCAGCAGCAGGTTCAGGCCTAC‑GTTGACACCAGATTCTTTTGCCGCTGCCGTGGTACCGCCAAGGGCAAAAATGTTCTTACCGAAg < 1:376895/238‑1 (MQ=255) gCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGc > 1:322080/1‑251 (MQ=255) caaccccccccccccccccgccgCTGACCGATCCCCCGCCTACCCCGCACCCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATCCGTCCGGCTTCAAACACCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGACCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTTTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCgg < 1:170661/234‑1 (MQ=255) cccgaccccACCCCGCCGCTGAACGATCCCCCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGtttt < 1:330336/246‑1 (MQ=255) gAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACg > 1:405284/1‑250 (MQ=255) ccGATACCCCGCCTCCCACGCCCGCCGCGATA‑GCACCCAGCTCATACATAAACCCGAGGTTGTGGGTGGCAGAGCCGATACGCCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGTTATTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAAc < 2:379582/250‑1 (MQ=255) gCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCa > 1:218548/1‑250 (MQ=255) aCCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCTCCAAAGTCATAGAACTCTCCAGACAACTCGTAGATCATCAGCAGGTTCATTCCGAC‑GTTGACACCAGATATTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACTT‑CACCGCAATc < 1:383972/249‑1 (MQ=255) cgcacgcCGCG‑TACGCATCCAGCTCATAAATAAAGCCGGGGTTGTGGGTGGCAGAGCCGATACGTCCGGCTTCTAACACCCCGCAAAAGGCAGAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACCCCAGATCCTTTTGCCGCTTCCGTGTTCCCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAAcgcg < 2:170442/251‑1 (MQ=255) cgccgcGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAAC‑CCCACGACGAACGC‑CACCGCAATCCACGCGt > 1:211712/1‑248 (MQ=255) cgccgcGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGACAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGt > 2:88124/1‑248 (MQ=255) gATA‑GCATCCAGCTCATACATAAACCCGAGGTTGTTGGGGGCAGAGCCGATACTTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACCCGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCATATACTTTGGCCCCTTCCGTTTTTCCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCTT‑GACGA‑CGTTAAGCGCAATCAACGCGTAGAAGGTGa < 1:24065/251‑1 (MQ=255) cacccAGCTCATACAAAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTGGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTaa < 1:404721/248‑1 (MQ=255) cATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGGTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCACcgcgaagcaggcgaggta > 1:115569/1‑236 (MQ=255) cAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGGGGAGAAGGGGATGTAag > 2:222033/1‑248 (MQ=255) gCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCACGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGGGATGTAagcg > 2:367484/1‑246 (MQ=255) aaaaacaaaaaGCCGAGTTTGTTGGGGACAGCGCCGATACGCCCGGCTACTAACACCCCGCCAAAGGCATCAACCACGCCAGAAAACGCGAAGATCATCAGCAGGTTCAGGCCGACTGTT‑ACACCAGATACTTTTGCCGCTTCCGTGGTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTGTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACg < 2:62046/243‑1 (MQ=255) cccgatttggtgggtggCAGAGCCGATACGCCCGGCTTCTAACACCCCGCCAAAGGCATAGACCACGCCAGAAAACGCGTAGACCATCAGCAGGTTCAGGCCGAC‑GTTGACCCCAGATCCTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTccc < 2:82313/237‑1 (MQ=255) cGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAgc > 1:93490/1‑251 (MQ=255) gttgttGGTGGCAGAGCCGATCCGTCCGGCTTCTAACATCCCTCCAAAGGCATAGAACCCGCCAGACAACGCGTAGATCATCAGCGGGTTCAGGCCGCC‑GTTGACTCCAGATCCTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGa < 1:85276/251‑1 (MQ=255) tgttgGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTccc > 1:82313/1‑241 (MQ=255) tgtgggtggCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCTTCAGCAGTATCAGGCCGCC‑GTTGACACCAGATACTTTTGCCGCTTCCTTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGAc < 1:426923/246‑1 (MQ=255) gATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAgg > 1:236169/1‑251 (MQ=255) tCTAACATCCCGCCAAAGGCATAGCACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGGTCTTACCGAAGCGGGTTTTGTTCCACAACACCAA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGCGATGTAAGAGAGACGGAAACTCCCCACCGCGACAAAGCCCTGCGCAAAGGGAAAGAAGCCACt > 2:307268/1‑251 (MQ=255) ccAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGACCGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGAGCGACAAAGCCCTGAGCAACGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGAc > 2:193465/1‑251 (MQ=255) aGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACa > 1:261348/1‑250 (MQ=255) cAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCAGCCAAAgg > 2:404996/1‑250 (MQ=255) aCGCGTAGATCATCGGCAGGTTCAGGCCGAC‑GTTGACACCAGATTCTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCg < 1:193465/251‑1 (MQ=255) ggtagacaatcaccggtttcggtcagAC‑GTGGACACAAGTTCCTTTTGCCGCTTCCTTGTTCCCGCCAATGGCAAAAATGTTCTTGCCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAAAGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGTCCCTACAAAGTCGTaa < 2:249922/226‑1 (MQ=255) cgTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGCCAAAGTCGTa > 2:320587/1‑250 (MQ=255) ccagcagGTTCAGGCCGCT‑GTTGACACCAGATCCTTTTCCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAg < 2:132638/250‑1 (MQ=255) ggTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATg > 1:328883/1‑251 (MQ=255) aGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCAt > 1:89984/1‑250 (MQ=255) gCCGAC‑TTTGACACCAGATACTTTTGCCGCTCCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAAGTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGa < 1:367484/251‑1 (MQ=255) cGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGTGTTACCGCCAATGGCCCAAATGTTCTTACCGAAGCGGGTTTTGTTCCACCACACCCA‑GACGCACGC‑CACCGCAATCAACGCGTCGAAGGTGATGTAAGAGCGACGGACACTCCCCAGCGCGACCAAGCCCTGAGCAAAGGTAGAAAAGCCACTGTCCAAACCAGAAATTGGCGACGCCCCGACAAAGTAGTAATAGAGCGCGTTGATGACATAGACg > 1:284688/1‑251 (MQ=255) ttGACGCCAGATCCTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACgatg < 1:77462/249‑1 (MQ=255) aTACTTTTGCCGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCg > 1:14374/1‑251 (MQ=255) aCTTATGCCGCTTCCGGGTAACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAg < 2:38651/251‑1 (MQ=255) ccGCTTCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGt > 2:31904/1‑250 (MQ=255) ttCCGTGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTACACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCAACTGTCAAACCCAGAAATTGGCGACGCCCCGACAACGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATg > 1:68490/1‑250 (MQ=255) tCCGTGTTACCGCCAATGGCAAAAATGTGCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCTAGCGTGGTAATGa < 2:61880/250‑1 (MQ=255) | GTAAAAATAATTACCCCGGTCACCACGCCAATCACCGTCCCCACACCGCCGCTGAACGATACACCGCCTACCACGCACGCCGCGATA‑GCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGAC‑GTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA‑GACGAACGC‑CACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGA > NC_000913/2236864‑2237350 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |