Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,224,169	A→G	F234F (TTT→TTC)	pbpG ←	peptidoglycan DD‑endopeptidase PbpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,224,169	0	A	G	100.0%	109.3 / NA	34	F234F (TTT→TTC)	pbpG	peptidoglycan DD‑endopeptidase PbpG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/21);  total (13/21)

CACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGC  >  NC_000913/2223935‑2224412                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    cACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCCTCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATCACCGTACGCATCCACAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGtt                                                                                                                                                                                                                                      >  2:371133/1‑250 (MQ=255)       aTCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGaa                                                                                                                                                                                                                               <  1:127124/251‑1 (MQ=255)            tCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATAtt                                                                                                                                                                                                                           <  1:393711/250‑1 (MQ=255)             cTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTcc                                                                                                                                                                                                                         >  2:177569/1‑251 (MQ=255)                   cGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACAGTACGCATCACCAGCCAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTAt                                                                                                                                                                                                                   >  2:353605/1‑251 (MQ=255)                               cgccgccATTTGGGCGGCTTTTTGTTTTTTATAGCTCCACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCACTCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTCGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACAcc                                                                                                                                                                                                        >  1:396374/1‑250 (MQ=255)                                        ttGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTa                                                                                                                                                                                               <  1:96388/250‑1 (MQ=255)                                        tggggCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTCCCAGCGCCACCGGTTTATTATTGATACCCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAgatgagta                                                                                                                                                                                               <  1:96385/248‑4 (MQ=255)                                         ggggCGGTTTTTTTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACAC‑AGATGATTAGt                                                                                                                                                                                             <  1:371133/249‑1 (MQ=255)                                          gggCGTCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACACGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGTCAAAATGGGTATATTTGCCAATCGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCTTACGCATCACCAGACAATGTCCCGCCGCAATGGTGAAGCCGGTTTTGTTTAACTGAATATTCCAGTTATCGCGATACACCCGATGATTAGTa                                                                                                                                                                                            <  2:165070/251‑1 (MQ=255)                                                 tttttGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTAGTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCgg                                                                                                                                                                                      <  2:42296/250‑1 (MQ=255)                                                   tttGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCATGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATGGCGGaa                                                                                                                                                                                    <  1:353605/250‑1 (MQ=255)                                                              taGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGtat                                                                                                                                                                         >  1:429936/1‑250 (MQ=255)                                                                      cGCTGCTGCCGGCACCGGCATCAATTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGAt                                                                                                                                                                >  2:94123/1‑251 (MQ=255)                                                                       gctgctGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGAtt                                                                                                                                                               <  1:177569/251‑1 (MQ=255)                                                                          gctgcCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGAtt                                                                                                                                                               >  2:331250/1‑248 (MQ=255)                                                                          gctgcCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTaga                                                                                                                                                            <  1:19489/251‑1 (MQ=255)                                                                                           cACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCt                                                                                                                                            <  2:112177/250‑1 (MQ=255)                                                                                                          aaTCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCTGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACtt                                                                                                                             >  1:310483/1‑250 (MQ=255)                                                                                                                 gTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGccc                                                                                                                     >  2:155248/1‑251 (MQ=255)                                                                                                                    cGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCCCTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGAt                                                                                                                  <  2:390449/251‑1 (MQ=255)                                                                                                                      cAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCa                                                                                                                <  1:9072/251‑1 (MQ=255)                                                                                                                                     ggCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGc                                                                                                 <  2:145472/251‑1 (MQ=255)                                                                                                                                             gggTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATg                                                                                         <  1:179519/251‑1 (MQ=255)                                                                                                                                                                gTCCATCACCACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGACGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCCGTTTGGTTAAGTCAc                                                                      >  2:182313/1‑251 (MQ=255)                                                                                                                                                                        cTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGt                                                                                   >  1:61738/1‑230 (MQ=255)                                                                                                                                                                        cTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGt                                                                                   <  2:61738/230‑1 (MQ=255)                                                                                                                                                                                gcCACCGGGTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTGCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGTGCAGTTGAAACGtt                                                      <  1:58368/251‑1 (MQ=255)                                                                                                                                                                                     cGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCa                                                 <  2:438469/251‑1 (MQ=255)                                                                                                                                                                                         ttattattaAAAACCGTACGAATCACCAGACAAGTGCCCGGCGCATTGGTGAAGCCGGTTTTGGTTATCATAATATTCCAGTTATCTCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGTGTAGTACTTAACTGCCCGATCAACGTATATTGTTTTCTGGCAATGAGCAGTTTTGTTAAGTCACGGGCAGTTTAAATGTTATGCACCg                                              <  2:48266/250‑1 (MQ=255)                                                                                                                                                                                          tattatTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGgta                                                                                                                                   <  1:370701/164‑1 (MQ=255)                                                                                                                                                                                          tattatTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGgta                                                                                                                                   >  2:370701/1‑164 (MQ=255)                                                                                                                                                                                                                     gccAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCgtg                 <  2:344953/249‑1 (MQ=255)                                                                                                                                                                                                                                    cATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGc  >  1:130173/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    CACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGC  >  NC_000913/2223935‑2224412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑