Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,177,670	G→A	L299F (CTC→TTC)	fbaB ←	fructose‑bisphosphate aldolase class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,177,670	0	G	A	100.0%	106.2 / NA	34	L299F (CTC→TTC)	fbaB	fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/19);  total (15/19)

TCGCATCAGGCATAGGTTGCCGGATGCAGCGTAAACGCCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGATCGTCGGTGTAACCGTAATTAATTGCTTTATAGCCGCCGTTATTTTCCGCCATTTTTTGTTTGACGATATCTGCACCGATGGTTGCCGCCAGATGGTTTGCCTGACCGGTCA  >  NC_000913/2177433‑2177906                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             gcGCATCAGGCATAGGTTGCCGGATGCAGCGTAAACGCCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAATTCAGTTTCAcc                                                                                                                                                                                                                                  <  2:266014/249‑1 (MQ=255)     aTCAGGCATAGGTTGCCGGATGCAGCGTAAACGCCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATGGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACAGCAGTACGCACTGCATCGCTGAAGTCAGTTTCAccgcccg                                                                                                                                                                                                                             >  2:133126/1‑251 (MQ=255)       cAGGCATAGGTTGCCGGATGCAGCGTAAACGCCTTATCCGTCCTACGGGGAGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCAccgcccgc                                                                                                                                                                                                                            >  1:177684/1‑250 (MQ=255)         ggCATAGGTTGCCGGATGCAGCGTAAACGCCTCATCCGTCCTACGGGGAGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCgcagc                                                                                                                                                                                                                         >  1:382128/1‑251 (MQ=255)                   gCCGGATGCAGCGTAAACGCCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAGCGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAg                                                                                                                                                                                                               <  2:116187/251‑1 (MQ=255)                                                     ggggTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAATTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAg                                                                                                                                                                             <  2:412016/251‑1 (MQ=255)                                                            ggttgttCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATACCCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTg                                                                                                                                                                      <  2:241111/250‑1 (MQ=255)                                                              ttgttCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCCTCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTCGCTAACTGAt                                                                                                                                                                    >  1:207948/1‑251 (MQ=255)                                                                gttCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCGGCACGGCGTTAATCAGTTTCCCGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAAGCAGCCCCATTCCTCCTGCGCGTTTTTTGATAACCGCAGTACGCACTGCATCTCTGAAGTCAGTTTCACCGCCCGCAGCGCCGCCGGAGTTAATCAACCCAGCCCGACCCATATAGCAGGGAGCTAACTGATaa                                                                                                                                                                  <  2:207948/251‑1 (MQ=255)                                                                        aaGGCGTATCAGGCTATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCATTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCTGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAg                                                                                                                                                          <  2:437847/251‑1 (MQ=255)                                                                        aaGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAg                                                                                                                                                          <  1:80423/251‑1 (MQ=255)                                                                        aaGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAg                                                                                                                                                          <  1:95540/251‑1 (MQ=255)                                                                               aTCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATc                                                                                                                                                   >  1:76932/1‑251 (MQ=255)                                                                                         aGTAATTTTGCTATCGGGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGAcc                                                                                                                                                                                           >  1:346052/1‑201 (MQ=255)                                                                                         aGTAATTTTGCTAGCGGGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGAcc                                                                                                                                                                                           <  2:346052/201‑1 (MQ=255)                                                                                               tttGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTc                                                                                                                                    >  1:70058/1‑250 (MQ=255)                                                                                                     aTCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCAACAGATCAATCGGGTTTTCGCTGGTCAATTTGc                                                                                                                             >  2:105378/1‑251 (MQ=255)                                                                                                        gagaTAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGt                                                                                                                          <  2:166370/251‑1 (MQ=255)                                                                                                                    cTGCACGGCGTTAATCAGTTTCCCGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTc                                                                                                                                    >  2:268946/1‑229 (MQ=255)                                                                                                                    cTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTc                                                                                                                                    <  1:268946/229‑1 (MQ=255)                                                                                                                      gCACGGCGTTAATCAGTTTCACGCCGCCAACCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGGTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAGCGCACCAGATCAATCTTGTTTGCGCTGGTCAATTTGCTGGAAACACGAtcgtcg                                                                                                            <  2:410359/251‑1 (MQ=255)                                                                                                                            cGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATGCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCCCTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGATCGTCGGTGTaa                                                                                                      <  2:231174/251‑1 (MQ=255)                                                                                                                                         cACGCCGTCAGCCACCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGATCGTCGGTGTAACCGTaattaatt                                                                                          <  2:37940/250‑1 (MQ=255)                                                                                                                                             ccGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGCTCGTCGGTGTAACCGTAATTAATTGCttt                                                                                     >  1:195557/1‑251 (MQ=255)                                                                                                                                                          aTTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGATCGTCGGTGTAACCGTAATTAATTGCTTTATAGCCGCCGtt                                                                         >  1:340840/1‑250 (MQ=255)                                                                                                                                                                 gAACTCTTTACGTCCAAGAATCAGCCCCAGTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTTCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGAt                                                                                                                                                        <  1:414821/164‑1 (MQ=255)                                                                                                                                                                 gAACGCTTTCAGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGCTAACCGTAGTACGCACTGCCTCGCTGAAGTCCGTTTCAACGCCCGCTGCACCGCCGGAGTTTATCACCCCAGTCCGCCCCATATAGCAGTTAGCTAACTGCTACCGCACCAGAt                                                                                                                                                        >  2:414821/1‑164 (MQ=255)                                                                                                                                                                        ttccttCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATACCCGTAGTACCCACTTCATCGCTGAAGTCATTTTCACCGCCCGCATCCCCGCCGGATTTTATCAACCCAGCCCGCCCCTTATAGCAGTTAGCTACCTGATAACGCCCCAGATCAATCGGGTTTTCGCTGGTAAATTTGCTGTAAACACGATCTTCGTTGTAACCGTAATTAATTGCTTTATAGCCGCCGTTATTTTCCGCCAttt                                                           <  2:344305/245‑1 (MQ=255)                                                                                                                                                                                                  gCCTGCGAGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCCCCGCCCGCAGCACCGCCGGCGTTCATCAACCCAGCCCGACCCATCTAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGATCGTCGGTGTAACAGTAATTAATTGCTTTATAGCCGCCGGTATTTTCCGACATTTTTTGTTTGACGATATCTGCACCGATgt                                >  2:191741/1‑250 (MQ=255)                                                                                                                                                                                                    cTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGAtcgtcg                                                                                                            >  2:103928/1‑173 (MQ=255)                                                                                                                                                                                                    cTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGAtcgtcg                                                                                                            <  1:103928/173‑1 (MQ=255)                                                                                                                                                                                                          gTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAAGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGATCGTCGGTGTAACCGTAATTAATTGCTTTATAGCCGCCGTTATTTTCCGCCATTTTTTGTTTGACGATATCTGCACCGATGGTTgccgcc                        >  1:113352/1‑251 (MQ=255)                                                                                                                                                                                                             tgttgAAAACCGCAGTCCCCCCTGCATCGCTGAAGTCCGTTTCCCCGCCCGCAGCACCGCCGGAGTTTATCACCCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCCCCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGATCGTCGGTGTAACCGTAATTAATTGTTTTATAGCCGCCGTTATTTTCCGCCATTTTTTGTTTGACGATATCTGCACCGATGGTTGCCGCCAg                      <  1:110077/250‑1 (MQ=255)                                                                                                                                                                                                                                cACTGCACCGCTGAATTCAGTTCCCCCGCCCGCAGCCCCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGATCGTCGGTGTAACCGTAATTAATTGCTTTATAGCCGCCGTTATTTTCCGCCATTTTTTGTTTGACGATATCTGCACCGATGGTTGCCGCCAGATGGTTTGCCTGACCGGTCa  <  2:113352/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             TCGCATCAGGCATAGGTTGCCGGATGCAGCGTAAACGCCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTGATAACGCACCAGATCAATCGGGTTTTCGCTGGTCAATTTGCTGTAAACACGATCGTCGGTGTAACCGTAATTAATTGCTTTATAGCCGCCGTTATTTTCCGCCATTTTTTGTTTGACGATATCTGCACCGATGGTTGCCGCCAGATGGTTTGCCTGACCGGTCA  >  NC_000913/2177433‑2177906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑