Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,146,017	C→T	L918F (CTT→TTT)	dgcE →	putative diguanylate cyclase DgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,146,017	0	C	T	100.0%	126.5 / NA	38	L918F (CTT→TTT)	dgcE	putative diguanylate cyclase DgcE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/15);  total (23/15)

CCGGGTGACGGTTTACGAACCGCAGCAAGCTGCCGCACATAGCGAACGGGCGGCGATGTCGCTTGATGAACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGCTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATAGCCCTCTACCACCACGGTTATTACATCTGATTATTCCGGCTGAAGCGATTTTAGATCACGCAGAAAGCGTGCAAAAACTGCGGCTGGCGG  >  NC_000913/2145779‑2146263                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       ccgggTGACGGTTTACGAACCGCAGCAAGCTGCCGCACATAGCGAACGGGCGGCGATGTCGCTTGATGAACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCAAGctc                                                                                                                                                                                                                                            >  2:311056/1‑251 (MQ=255)   gggTGACGGTTTACGAACCGCAGCAAGCTGACGCACATAGCGAACGGGCGGCGATGTCGCTTGATGAACAGAGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCCCGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGATGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTAAGCGATCCGGCGTTTAGCCATGCTCtt                                                                                                                                                                                                                                          >  1:395844/1‑251 (MQ=255)    ggTGACGGTTTACGAACCGCAGCAAGCTGCCGCACATAGCGAACGGGCGGCGAGGTCGCTTGATGAACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACCGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTTCGAAGGCGAGATTATTGATGACCAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCttt                                                                                                                                                                                                                                         >  1:83285/1‑250 (MQ=255)                     cGCAGCAAGCTGCCGCACATAGCGAACGGGCGGCGCTGTCGCTTGGTGACCAGTGGCGGCTGATTAAAGAGAATCAGTTGCTGATGCTCGCCCCAGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGCTTTCACTTAAGATCTGGAGGTGCGAAGGCGCGCTTATTGATGAACAAACATTTCGTCGTAGCTTCAGCCGTCCGGTGTTTAGCCATGCTCTTGACCCGCGGGTATTcccc                                                                                                                                                                                                                        >  2:229833/1‑249 (MQ=255)                       cagcaAGCTGCCGCACATAGCGAACGGGCGGCGATGTCGCTTGATGAACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTAGCCCACGGTGTCGCTTCGCCACGGATCCCGGATGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGGATTCCACg                                                                                                                                                                                                                       >  1:391551/1‑250 (MQ=255)                         gcaAGCTGCCGCACATAGCGAACGGGCGGCGATGTCGCTTGATGAACAGTGGCGGATGAGTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAAt                                                                                                                                                                                                                    <  2:142252/251‑1 (MQ=255)                           aaGCTGCCGCACATAGCGAACGGGCGGCGATGTCGCTTGATGAACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAAttt                                                                                                                                                                                                                  <  2:117642/251‑1 (MQ=255)                                   gCACATAGCGAACGGGCGGCGATGTCGCTTGATGAACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCCGCGATCCGGCGTTTCGACCTGCTCTTGACCGCCGGGTATTCCACGAATTTTTCcagcc                                                                                                                                                                                                          >  1:368837/1‑250 (MQ=255)                                              aCGGGCGGCGATGTCGCTTGATGAACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCaaa                                                                                                                                                                                                 >  1:68122/1‑249 (MQ=255)                                                        aTGTCGCTTGATGAACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCGGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAg                                                                                                                                                                                     <  1:83841/251‑1 (MQ=255)                                                          gTCGCTTGATGAACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGAAAAAGCGGGTGCCAGt                                                                                                                                                                                    >  1:311246/1‑250 (MQ=255)                                                              cTTGATGAACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGCTTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAACAGCGGTTGCCAGTAAAgg                                                                                                                                                                               >  1:396322/1‑251 (MQ=255)                                                                tgatgaACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAgg                                                                                                                                                                               <  2:217767/249‑1 (MQ=255)                                                                 gatgaACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCAt                                                                                                                                                                            >  1:282707/1‑251 (MQ=255)                                                                                      aaaGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCttt                                                                                                                                                        <  2:368837/250‑1 (MQ=255)                                                                                       aaGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTcc                                                                                                                                                      <  2:197949/251‑1 (MQ=255)                                                                                              tCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCg                                                                                                                                               >  1:151655/1‑251 (MQ=255)                                                                                                   tgatgaTGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGttt                                                                                                                                           <  2:151655/250‑1 (MQ=255)                                                                                                       gatgCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCACCCCCTTTCCGTTGCCGGTTTGAGTa                                                                                                                                      <  2:83285/251‑1 (MQ=255)                                                                                                       gatgCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCCGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGt                                                                                                                                       >  2:332095/1‑250 (MQ=255)                                                                                                       cgagCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTa                                                                                                                                      <  2:384997/248‑1 (MQ=255)                                                                                                             cGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTa                                                                                                                                      >  1:384997/1‑245 (MQ=255)                                                                                                             cGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGGAGCGCCa                                                                                                                                <  2:217693/251‑1 (MQ=255)                                                                                                              gCCCACGGGGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAACGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGCGTAGCGCCAc                                                                                                                               >  2:370016/1‑251 (MQ=255)                                                                                                                               gCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGc                                                                                                              >  1:378088/1‑251 (MQ=255)                                                                                                                                                                   ttCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGCGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCCGCTGGAAAATAGCCCTCTACCACCACGGt                                                                          >  2:370208/1‑251 (MQ=255)                                                                                                                                                                      aCTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATAGCCCTCTACCACCACGGttat                                                                       >  2:188762/1‑251 (MQ=255)                                                                                                                                                                           aGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATAGCCCTCTACCACCACGGTTATTACAt                                                                  <  2:282707/251‑1 (MQ=255)                                                                                                                                                                                                agaTTATTGATGCACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCAGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATCGCCCACTACCACCACGGTTATTACATCTGATTATTCCGGCTGAAGCg                                             >  1:358325/1‑251 (MQ=255)                                                                                                                                                                                                 gaTTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATAGCCCTCTACCACCACGGTTATTACATCTGATTATTCCGGCTGAAGCg                                             >  2:143376/1‑250 (MQ=255)                                                                                                                                                                                                  attattGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTccccc                                                                                                                                                           <  1:64412/139‑1 (MQ=255)                                                                                                                                                                                                  attattGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTccccc                                                                                                                                                           >  2:64412/1‑139 (MQ=255)                                                                                                                                                                                                    tattGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATAGCCCTCTACCACCACGGTTATTACATCTGATTATTCCGGCTGAAGCGAtt                                          <  1:311056/250‑1 (MQ=255)                                                                                                                                                                                                                      tcgtcgTAGCTTCAGCGATCCGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATAGCCCTCTACCACCACGGTTATTACATCTGATTATTCCGGCTGAAGCGATTTTAGATCACTCAGAAAGc                        <  2:271626/250‑1 (MQ=255)                                                                                                                                                                                                                                          cGGCGTTTAGCCATGCTCTTGACCGTCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATAGCCCTCTACCACCACGGTTATTACATCTGATTATTCCGGCGGAAGCGATTTTAGATCACGCAGAAAGCGTGCAAATTCTGCGGCTGGCg   <  1:360670/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATACGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATAGCCCTCTACCACCACGGTTATTACATCTGATTATTCCGGCTGAAGCGATTTTAGATCACGCAGAAAGCGTGCAAAAACTGCGGCTGGCg   >  1:184407/1‑251 (MQ=255)                                                                                                                                                                                                                                          cGGCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATAGCCCTCTACCACCACGGTTATTACATCTGATTATTCCGGCTGAAGCGATTTTAGATCACGCAGAAAGCGTGCAAAAACTGCGGCTGGCg   >  1:209119/1‑251 (MQ=255)                                                                                                                                                                                                                                           ggCGTTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATAGCCCTCTACCACCACGGTTATTACATCTGATTATTCCGGCTGAAGCGATTTTAGATCACGCAGAAAGCGTGCAAAAACTGCGGCTGGCgg  >  2:339854/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       CCGGGTGACGGTTTACGAACCGCAGCAAGCTGCCGCACATAGCGAACGGGCGGCGATGTCGCTTGATGAACAGTGGCGGATGATTAAAGAGAATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAAGCTCTGGAGTTGCGAAGGCGAGATTATTGATGAACAAACATTTCGTCGTAGCTTCAGCGATCCGGCGCTTAGCCATGCTCTTGACCGCCGGGTATTCCACGAATTTTTCCAGCAGGCCGCAAAAGCGGTTGCCAGTAAAGGCATAAGCATCTCCCTCCCCCTTTCCGTTGCCGGTTTGAGTAGCGCCACGCTGGTGAATGATCTGCTTGAGCAGCTGGAAAATAGCCCTCTACCACCACGGTTATTACATCTGATTATTCCGGCTGAAGCGATTTTAGATCACGCAGAAAGCGTGCAAAAACTGCGGCTGGCGG  >  NC_000913/2145779‑2146263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑