Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,202	G→A	intergenic (‑20/‑150)	yoaI ← / → yeaL	protein YoaI/DUF441 domain‑containing inner membrane protein YeaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,202	0	G	A	100.0%	94.9 / NA	30	intergenic (‑20/‑150)	yoaI/yeaL	protein YoaI/DUF441 domain‑containing inner membrane protein YeaL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/13);  total (17/13)

TTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATT  >  NC_000913/1873964‑1874435                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          tttGATGAAATCGCCTTCACTGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGCACGGACAAAACAAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTTTCGACAAACATTTGATCGTTCATAGCATGACCCCGGGAGATAACTGTTTTCACGt                                                                                                                                                                                                                               >  1:378679/1‑251 (MQ=255)   tgatgaAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGtt                                                                                                                                                                                                                              >  1:264258/1‑250 (MQ=255)     atgaAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTAtt                                                                                                                                                                                                                           <  2:13167/251‑1 (MQ=255)                 tCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGca                                                                                                                                                                                                                <  2:190829/250‑1 (MQ=255)                      gCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTTATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAAt                                                                                                                                                                                                           <  1:437059/250‑1 (MQ=255)                                 ggATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAGCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTtgatg                                                                                                                                                                                               <  1:426681/251‑1 (MQ=255)                                   aTAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGAc                                                                                                                                                                                             >  2:24454/1‑251 (MQ=255)                                   aTAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGAc                                                                                                                                                                                             >  1:363012/1‑251 (MQ=255)                                      aaTCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGt                                                                                                                                                                                          >  1:430427/1‑251 (MQ=255)                                       aTCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGtt                                                                                                                                                                                         >  2:283561/1‑251 (MQ=255)                                          cGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGc                                                                                                                                                                                      >  2:304290/1‑251 (MQ=255)                                                 ttGAAAACCGCGGATTATTTGCGCATTGCGCAACCATAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAtt                                                                                                                                                                                <  2:115866/250‑1 (MQ=255)                                                              attattTGCGCATTGCGCAACCAGAACTGGTCAATTTTCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCaaaaa                                                                                                                                                                   >  1:84687/1‑250 (MQ=255)                                                                      cgcattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGa                                                                                                                                                          <  1:283561/251‑1 (MQ=255)                                                                                 aacAGAACTGGTCAATTTCAGCCGCACTGCGTCAATAGCCGGTCCGTTCGATAAGAAGAACGGACCAACCCAGTACAACCGCAATGGCAAACAATGATGCCGTGATACTCAGTGTCTCGACAAACATTTGATCGTTCATCGCCTGCCCCCGGGATCTAACTGTTTTCACGTTATTGTTAGAGAGCACACATGGCAGTTTGATGACAGTTCGCCTGATTTTTTTTGCAAAAAACGTCCAGATTTTCTCctt                                                                                                                                                >  2:374155/3‑250 (MQ=255)                                                                                       aCTGGGCAATTGCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGGTAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATGGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTgccttcct                                                                                                                                         <  2:180506/251‑1 (MQ=255)                                                                                              aaTTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTgcagca                                                                                                                                   >  1:194631/1‑250 (MQ=255)                                                                                                                        cGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAAGGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTAttt                                                                                                        <  1:98313/251‑1 (MQ=255)                                                                                                                         ggTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACTAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAATCAGGCTTATTAtttt                                                                                                       <  2:180780/251‑1 (MQ=255)                                                                                                                         ggTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTattatt                                                                                                         <  1:437094/249‑1 (MQ=255)                                                                                                                         ggTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTattatt                                                                                                         >  2:437094/1‑249 (MQ=255)                                                                                                                                                     aCCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGt                                                                           >  1:121659/1‑251 (MQ=255)                                                                                                                                                     aCCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGAATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGt                                                                           >  1:193791/1‑251 (MQ=255)                                                                                                                                                              acaGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGTAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCt                                                                  >  1:55522/1‑251 (MQ=255)                                                                                                                                                                           aaaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCggg                                                     <  1:102366/251‑1 (MQ=255)                                                                                                                                                                                          tAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGc                                      >  1:66171/1‑251 (MQ=255)                                                                                                                                                                                          tAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGc                                      >  1:109314/1‑251 (MQ=255)                                                                                                                                                                                                  gtCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAg                              <  2:109314/251‑1 (MQ=255)                                                                                                                                                                                                                     cGTTCATAGCATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGcc           <  2:194631/251‑1 (MQ=255)                                                                                                                                                                                                                              cATGCCCCCGGGAGATAACTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAAtt  >  1:273493/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          TTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATT  >  NC_000913/1873964‑1874435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑