Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,861,827	C→T	A50T (GCC→ACC)	yeaC ←	DUF1315 domain‑containing protein YeaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,861,827	0	C	T	100.0%	67.9 / NA	23	A50T (GCC→ACC)	yeaC	DUF1315 domain‑containing protein YeaC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/20);  total (3/20)

GGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATCGTTTTTTCAACCGTTGATTTCTTCGCCGTTTTCGCCATCGGTAAAGCGTAAAGAGGCAGAGTTA  >  NC_000913/1861585‑1862073                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        gggACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCcaca                                                                                                                                                                                                                                                <  2:379132/251‑1 (MQ=255) gggACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCcaca                                                                                                                                                                                                                                                <  1:62442/251‑1 (MQ=255)       gCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGAGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATCCCCAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑gtctcggcctcc                                                                                                                                                                                                                                          >  1:276859/1‑240 (MQ=255)                                                   tGAGAGGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTcaacgcaac                                                                                                                                                                                               <  2:186646/250‑1 (MQ=255)                                                    gagaTGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTcaacgcaacg                                                                                                                                                                                              <  2:98109/250‑1 (MQ=255)                                                    gagaTGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTcaacgcaacg                                                                                                                                                                                              <  1:275357/250‑1 (MQ=255)                                                                ggggTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTTTGCC‑CAGCCTCCCCCGTTGCCGGCGGTTTTTTTTTTTTTTCTCGGGCAACGCAAAGccctccagcccat                                                                                                                                                                                 >  1:194473/1‑240 (MQ=255)                                                                         gttgGCTATCCCCTTAATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGGGCTTCGATATTATGGCGCGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCGTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTGCCCCAGtt                                                                                                                                                                        <  2:276859/251‑1 (MQ=255)                                                                                 tCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑TCCCCAGCCTCACCCGCTTCAGGGAGTTTTCTTTTTTTTTGTCGGTCAACGCACCACCCCCAGGCCATTTTCCCCGttttccggcg                                                                                                                                                                >  1:68929/1‑243 (MQ=255)                                                                                   cccTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGt                                                                                                                                                              <  2:119968/251‑1 (MQ=255)                                                                                              cGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCt                                                                                                                                                   <  1:348790/251‑1 (MQ=255)                                                                                                  gCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGta                                                                                                                                               <  1:359478/251‑1 (MQ=255)                                                                                                         ggggATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCGGGTATACCTCa                                                                                                                                        <  1:95953/251‑1 (MQ=255)                                                                                                                aaCCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCAGCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGcatca                                                                                                                                 <  2:75457/251‑1 (MQ=255)                                                                                                                                  ggCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCATCGCCAGCAGGCCATTTCCCCAGTTCAACGGCGGACGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGtca                                                                                                                <  1:393533/250‑1 (MQ=255)                                                                                                                                  ggCAATAGGCTTTGCTGAGATACCAAAATCGTCTTTGAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGtcat                                                                                                               <  1:404780/251‑1 (MQ=255)                                                                                                                                                                 tcttTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCtgtg                                                                                   <  1:186948/248‑1 (MQ=255)                                                                                                                                                                                           gACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATaa                                                                                                                     <  1:213208/188‑1 (MQ=255)                                                                                                                                                                                                   gACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATCGTTTTTTCAACCGTTGAtt                                               <  1:194497/250‑1 (MQ=255)                                                                                                                                                                                                               cAATTGTCATATGCTGTGCTTCGATATTATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATCGTTTTTTCAACCGTTGATTTCTTCGCCGttt                                   <  1:53370/250‑1 (MQ=255)                                                                                                                                                                                                                         atGCTGTGCTTCGATATGATGGCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAAGTTTTCCGCTCCTGTGGAATAAGTTGCTGAATCGTTTGTTCAACCGTTTATTTCTTCGCCGTTTTCGCCATCgg                         <  2:362549/250‑1 (MQ=255)                                                                                                                                                                                                                                             ggCGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATCGTTTTTTCAACCGTTGATTTCTTCGCCGTTTTCGCCATCGGTAAAGCGTAAAGAGGCAGAGt    <  2:194473/251‑1 (MQ=255)                                                                                                                                                                                                                                               cGGGTTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATCGTTTTTTCAACCGTTGATTTCTTCGCCGTTTTCGCCATCGGTAAAGCGTAAAGAGGCAGAGTTa  <  1:320588/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        GGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTT‑GCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATCGTTTTTTCAACCGTTGATTTCTTCGCCGTTTTCGCCATCGGTAAAGCGTAAAGAGGCAGAGTTA  >  NC_000913/1861585‑1862073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑