Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,729,510	A→G	K142E (AAA→GAA)	lhr →	ATP‑dependent helicase Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,729,510	0	A	G	100.0%	156.4 / NA	52	K142E (AAA→GAA)	lhr	ATP‑dependent helicase Lhr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/26);  total (26/26)

ACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTG  >  NC_000913/1729277‑1729755                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      aCGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAACAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTCATccg                                                                                                                                                                                                                                      >  1:357778/1‑251 (MQ=255) aCGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATcc                                                                                                                                                                                                                                       <  1:99060/250‑1 (MQ=255)   gCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATccgcc                                                                                                                                                                                                                                    >  2:114694/1‑251 (MQ=255)     ccTCGATCGGCGCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCgg                                                                                                                                                                                                                                  <  2:434038/251‑1 (MQ=255)             ggCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTg                                                                                                                                                                                                                          >  1:45971/1‑251 (MQ=255)                 ctTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATc                                                                                                                                                                                                                       >  1:264439/1‑250 (MQ=255)                               gcgAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCACGATATTGCACCGATAAAAGCCCTGGGCACCGACGTGCAGCGCAACTTGCAGATCCCGGTGAAGGGTAGTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACGCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCa                                                                                                                                                                                                        <  2:170821/251‑1 (MQ=255)                                             gAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCt                                                                                                                                                                                          <  1:370898/251‑1 (MQ=255)                                              aGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATCAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTg                                                                                                                                                                                         >  1:163290/1‑251 (MQ=255)                                              aGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATACAAGCCCTGGTCACCGACGTTCAGCGCAACTTTCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACATGAACGCAGCGAACTCCCCCGTAATCCGTCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTg                                                                                                                                                                                         <  2:384402/251‑1 (MQ=255)                                                gcgcATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGAc                                                                                                                                                                                       >  1:421574/1‑251 (MQ=255)                                                  gcATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGatat                                                                                                                                                                                                                              <  2:304146/210‑1 (MQ=255)                                                  gcATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGatat                                                                                                                                                                                                                              >  1:304146/1‑210 (MQ=255)                                                       aGCGTAACACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCACGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTCCTGGCGCTACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCcgc                                                                                                                                                                                >  2:433471/1‑251 (MQ=255)                                                         cGTAAAACCTCACGCATCCTCTATATGTCACCGATAAAAGCCCTGGTCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCTCCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTTATGCTGACCTCTcgcgc                                                                                                                                                                              <  1:134392/251‑1 (MQ=255)                                                             aaaCCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCCGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGCATCACTCTATCTGATGCTGACCTCCcgcgcgcgc                                                                                                                                                                          >  2:206728/1‑251 (MQ=255)                                                               aCCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCcgcgcgcgcg                                                                                                                                                                         >  1:54705/1‑250 (MQ=255)                                                                 cTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGAAGCGAACTCACCCGTAATCCGCCGGATATTCTTATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGaa                                                                                                                                                                       >  1:304415/1‑250 (MQ=255)                                                                   cccgcgtCCTCTATATTGCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGGTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGaaa                                                                                                                                                                      <  2:5260/243‑1 (MQ=255)                                                                       cATCCTCTATCTTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGCATTGCCGATGAACGGCGGCGGCGCGGCGAACCGGTAGTCCATCTTCGCGTAGGGAACCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGAGAAACGCTAc                                                                                                                                                                >  2:271931/1‑251 (MQ=255)                                                                                          aTAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAat                                                                                                                                             <  1:409608/251‑1 (MQ=255)                                                                                             aaaGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAattat                                                                                                                                          <  1:319923/251‑1 (MQ=255)                                                                                               aGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtg                                                                                                                                        <  1:332320/251‑1 (MQ=255)                                                                                                  ccTGGGCCCCGACGTTCAGCGCAACTTGCAGAGCCCGGTGAATGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGAGACGCCTGCACAGGAACGCAGCGAACTCTCCCGTAATCCGCCGGATAGTCTGATCACCACGCCCGAATCACTCTATCTGATGCTGACCGCCCGCGCGCGCGAAACGCTACGCTGCGTCGAAACGTTAATTATtgatg                                                                                                                                     <  2:304415/251‑1 (MQ=255)                                                                                                  ccTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGCATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgatg                                                                                                                                     >  1:159690/1‑251 (MQ=255)                                                                                                   cTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgatg                                                                                                                                     >  1:282377/1‑250 (MQ=255)                                                                                                     gggCGCCGCCGTTCAGCGCAACTTGCAGAGACCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTGCGCGTAGGGAGCCGTACTGGCGATACGCCTGCACAGGAACGCATCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTTCCGCGCGCGCGAAACGCTACGCGGCTTCGAACCGGTAATTATTGATGaa                                                                                                                                   <  2:72281/250‑1 (MQ=255)                                                                                                                         aCTTGCAGATCCCGTTGAAGGGTATTGCCGAGGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGt                                                                                                              <  2:236888/251‑1 (MQ=255)                                                                                                                          cTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACACGTAATCCGCCGGATATTCTGATCACCCCACCCGAATCACTCTATCTGCTGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGt                                                                                                              >  1:250655/1‑250 (MQ=255)                                                                                                                            tGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTa                                                                                                             <  1:248990/249‑1 (MQ=255)                                                                                                                             gCAGATCCCGTGGAAGGGTAGTGCCGATGAGCGGCGGCGGAGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGAGCCGTACTGGCGATACGCCTTCACAGGAACTCAACGAACTCACCCTTAATCCGCCGGATATTCTGATCGCCACGCCCGAAGCACTCTATCTGTTGCGGACCTCCCGCGCGCGCGAAAGGCTACGCGTCGTCTAAACGGTAATAATGGAGGAAGGCCACGCGTTAGCAGGCAGTAAAc                                                                                                          <  2:44208/251‑1 (MQ=255)                                                                                                                                 aTCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAACCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTACCGCGCGCGCGAAACGCTACGCGGCGTCGAAAAGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgggg                                                                                                      >  2:109301/1‑248 (MQ=255)                                                                                                                                            gggTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTgg                                                                                           <  2:424780/251‑1 (MQ=255)                                                                                                                                                 ttGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGtt                                                                                       >  2:376612/1‑250 (MQ=255)                                                                                                                                                      gATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCt                                                                                 >  1:296809/1‑251 (MQ=255)                                                                                                                                                             ggcggcggcGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCACGAAACGCTCCGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTACGTCTGGAGCgg                                                                          >  2:261289/1‑251 (MQ=255)                                                                                                                                                                   ggcgcggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCTCTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGAt                                                                    <  1:137293/251‑1 (MQ=255)                                                                                                                                                                   ggcgcggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGAt                                                                    >  1:315994/1‑251 (MQ=255)                                                                                                                                                                      gcggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGCTATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGACACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGCTgcg                                                                 >  2:343981/1‑251 (MQ=255)                                                                                                                                                                      gcggcgAAACGGAAGTCAATCTCCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgcg                                                                 <  2:321986/251‑1 (MQ=255)                                                                                                                                                                                ggAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTCTCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCcacc                                                       >  1:182741/1‑250 (MQ=255)                                                                                                                                                                                          cTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCTGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCAGCGCGAGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGCTGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGCTGCGCTGCTCCACACCTCAGcaa                                              >  2:438838/1‑249 (MQ=255)                                                                                                                                                                                            tCGCGTAGGGATCCGTCCGGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGGAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGGCTGGACCGGCTAGATGCGCTGCTCCACACCTCAGcac                                              <  2:285946/248‑1 (MQ=255)                                                                                                                                                                                            tCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAg                                            <  1:114694/250‑1 (MQ=255)                                                                                                                                                                                                gTAGGGATCCGTACTGGCGATACGCCTGCACCGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGACACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACCCCTCAGCACAGCGAAt                                       >  2:373548/1‑251 (MQ=255)                                                                                                                                                                                                  aGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTg                                     >  2:218198/1‑251 (MQ=255)                                                                                                                                                                                                     gATCCGGACTGGCGATACGCCTGCACAGGAACGCAGCGAACGCACCCTTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAATCGGTAATTATTGATGAAGTCCACGCGGTAGCTGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCTGCACAGCGAATTGGcc                                  <  2:326321/251‑1 (MQ=255)                                                                                                                                                                                                      aTCCGTACTGGCGATACTCTTGCACAGGAACGCCGCGAACTCCCCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTACACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCCCAGCGAATTGGCCt                                 <  2:373450/251‑1 (MQ=255)                                                                                                                                                                                                         cGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTc                              <  2:250655/251‑1 (MQ=255)                                                                                                                                                                                                                       gCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCt                <  2:315994/251‑1 (MQ=255)                                                                                                                                                                                                                        ccTGCCCAGGAACGCCGCGCACTCACCCGTACTCCGCCGGCTATTCTGAACACCACACCCGAATCACTCTATATGATGCTGACCTCCCGCGAGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTACGTCTGGAGAGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTc               >  2:20686/1‑251 (MQ=255)                                                                                                                                                                                                                                  aaCGCAGCGAACTCACCCGTAAGCCGCCGGATATTCTGATCACCACACCCGAATCCCGCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTAATGAAGGCCACGCGGTAGCGGGCAGTAAGCGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGTCCTTTCTGCCACTGTGGGCTCAGCCAGCGAt     <  1:373548/251‑1 (MQ=255)                                                                                                                                                                                                                                     gcagcgAACTCACCCGTATTCCGCCGGATATTCTCATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAACCGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCCCCCCTCAGCACAGCGAATTGGCCTTTCTGCCCCTTGGCGCTCCGCCAGCGAtgtg  >  2:157969/1‑251 (MQ=255)                                                                                                                                                                                                                                     gcagcgAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCGGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTGGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGAtgtg  <  2:314146/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      ACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTG  >  NC_000913/1729277‑1729755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑