Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	42.2 / NA	15	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/5);  total (10/5)

CCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652118‑1652575                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       ccTGGTGAGCTGAGTGCACTTGGATGGGAGGACATAGATCTGAAAACTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGGCATTACTCTTATTAAGCCACCAATAGACGCTCTCCGGAATCAGATGAAATTTACAGGACTTAGCAAAGAGCATCTCCATGATGTTTACCTCAGAAAGTATGGCAGAAACA‑AAAAACAAAAAtaaac                                                                                                                                                                                                                  >  2:419921/1‑247 (MQ=255)                       gATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATgg                                                                                                                                                                                                                                           <  1:18202/205‑1 (MQ=255)                       gATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAACCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATCTCCTTGATGTTCACCTCCGAGAGTAttg                                                                                                                                                                                                                                           >  2:18202/1‑203 (MQ=255)                                  aTAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCGCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTTATGTTAACCTCAGAGAGTATGGCAGAA‑AAGAAAAACAAAAATGCACCTTTGTTTTTCAAACTGAA‑GTGTCAGCgag                                                                                                                                                                                   >  2:339208/1‑248 (MQ=255)                                                            gatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATTGTGACCATTTTACCg                                                                                                                                                      >  1:331929/1‑251 (MQ=255)                                                                           aGCAAAAGACCGTTTCACTGTACCAAAAACACAGGCGGGAACCAATCGGGTCCTTCATCTTTTTAATCCACCAATCGAAGCTCTCCGGGGTCCGATGACATTAACGTGACTGAGCAAAGAGCATATCATTGATGTTCCCCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCCCCTTTGTTTTTTAACCTGAC‑ATGTCCGCGCGAGTAAAAAATTATGGTGACCCTTTTACCGTTGACTCAATaaagc                                                                                                                                       >  1:128671/1‑248 (MQ=255)                                                                             cAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTGTCAACCGGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAAGAAGGCAg                                                                                                                                     <  2:277871/251‑1 (MQ=255)                                                                                      gTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAGCCAGCAAACGACGCTCCCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGCATATCCTTGATGTTCCCCTCAGAGAGTATTGCAGAA‑CAGAAAAAAAAAAATGCACCTTTGTTTTTCAAACTGAAAGTG‑CAGCGAGAGTAAAAAAATATGGTGACCCTTTTAACGTTGGCTCAATAAAGGAGATGGGGGAc                                                                                                                            >  2:304027/1‑251 (MQ=255)                                                                                                               gggAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTcc                                                                                                   >  1:133865/1‑251 (MQ=255)                                                                                                                                       tattaAGCCAGCAATCGACGCTCTCCAGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGt                                                                              <  1:91997/248‑1 (MQ=255)                                                                                                                                        attaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCga                                                                           <  1:304027/250‑1 (MQ=255)                                                                                                                                                   aaTCGACGCTCTCCGGAGTCAGATGAAATTAACGAGACTGAGCAACGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACAGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATg                                                               >  2:209235/1‑251 (MQ=255)                                                                                                                                                                                 aaCGAGACTGAGCAAAGAGCATATCATTGATGTTAACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTaaac                                 >  2:333385/1‑249 (MQ=255)                                                                                                                                                                                       aCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGGTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTTACAGCTGGTGCTAACCCGGCAt                           <  1:134182/251‑1 (MQ=255)                                                                                                                                                                                                                gTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGc  >  1:10989/1‑251 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652118‑1652575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑