Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,574	C→T	A87V (GCC→GTC)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,574	0	C	T	100.0%	97.5 / NA	32	A87V (GCC→GTC)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/22);  total (10/22)

GAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATG  >  NC_000913/4627335‑4627809                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            gAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGCCCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATGCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGTGTCTGTAGCCAgg                                                                                                                                                                                                                                  <  2:295174/251‑1 (MQ=255)  aaaaCTGCCCTCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGt                                                                                                                                                                                                                                 >  2:228843/1‑251 (MQ=255)                   aCAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGTTGTCGTATTCTGTAAGTTCTACCCACTGCATACCGTACAGATCTACCGTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCa                                                                                                                                                                                                                <  2:288070/251‑1 (MQ=255)                           gCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATggg                                                                                                                                                                                                         <  1:364888/250‑1 (MQ=255)                                        ggTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAAGCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGGTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATAccc                                                                                                                                                                                           <  2:173801/251‑1 (MQ=255)                                                         gTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCGCCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAATTGCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGCGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCTCGTTGTTc                                                                                                                                                                          <  2:357586/251‑1 (MQ=255)                                                           aTGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTGCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCg                                                                                                                                                                         <  2:162393/250‑1 (MQ=255)                                                                 aaaGGGTATTTAAGCCAGATACTGAATGCCAAAATCAACAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTTACGATACCCGTGACCGCGCGTTGTTCgacgac                                                                                                                                                                    >  2:73641/1‑249 (MQ=255)                                                                        aTTTAAGCCAGTTACTGAATGCCACAATCAAAAGCCCCAGCGCTCAAAAGCTGGAGGCGTTGCACCGTTTTTTGAGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGcc                                                                                                                                                                                                                                     >  2:230571/1‑177 (MQ=255)                                                                        aTTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCCACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGcc                                                                                                                                                                                                                                     <  1:230571/177‑1 (MQ=255)                                                                            aaGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCg                                                                                                                                                        <  1:114639/250‑1 (MQ=255)                                                                             aGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgc                                                                                                                                                       >  1:380779/1‑250 (MQ=255)                                                                                     aCTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGa                                                                                                                                              <  1:176985/251‑1 (MQ=255)                                                                                        gAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATTCCGGACATATCTACCGTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAATACAGTGCCATGTCGCAGCAGCCGACCg                                                                                                                                           <  2:375015/251‑1 (MQ=255)                                                                                                       aaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTCCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGc                                                                                                                            <  2:236195/251‑1 (MQ=255)                                                                                                         gCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGAGCGTCTGCGt                                                                                                                          <  2:145649/251‑1 (MQ=255)                                                                                                            ccAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTgg                                                                                                                       <  1:218733/251‑1 (MQ=255)                                                                                                             cAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGt                                                                                                                      >  1:3339/1‑251 (MQ=255)                                                                                                              aGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGtt                                                                                                                     >  1:246391/1‑251 (MQ=255)                                                                                                                   gcAAAAGCTGGATGCGTTGCACCGTTTTTTTGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATTGGTGTCGTATTCGTTAAGTTCTACCCACTGCATACCGGACATATCTACCTTAACCAGCGCGTCTGGAGCCAGGTTGACTAGCTGCATATCATTATGGTTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGGTATTGc                                                                                                                <  1:256816/251‑1 (MQ=255)                                                                                                                    cAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGc                                                                                                                >  2:187644/1‑250 (MQ=255)                                                                                                                       aaGCTGGAGGCGTTGCACCGATTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGAGCGTTGTTCGAAGACAGTGCCATGTCGCCGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAAc                                                                                                            >  2:369272/1‑251 (MQ=255)                                                                                                                             gAGGCGTTGCACCGTTTTTTGGGGCTTGAGGTTCCCCGGTAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACATTGCCATGTCGCATCAGGCGATCGTGCCGGATCGTCTGCGTTGGTGATTTCAAACTTTTaa                                                                                                      <  2:45875/251‑1 (MQ=255)                                                                                                                              gggCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCCGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTaaa                                                                                                     <  2:78310/250‑1 (MQ=255)                                                                                                                                gCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAata                                                                                                   <  2:380779/251‑1 (MQ=255)                                                                                                                                     gCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGACAACTTTTAAATATc                                                                                                 >  2:140604/1‑248 (MQ=255)                                                                                                                                       aCCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGaaa                                                                                            <  2:3339/251‑1 (MQ=255)                                                                                                                                            tgtttGGGGCTTGAGTTTCCCCGGCAGAATAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGGGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAata                                                                                        <  2:438958/248‑1 (MQ=255)                                                                                                                                                                         gaaAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCa                                                          <  1:228843/251‑1 (MQ=255)                                                                                                                                                                                                     ttCTACCCACTGCATAACGACCATATCTACCTTATCCAGCGCGTCTATAGCCAGGTTGACGAGCTGCATATCATTATGGGCTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCACAGCAGCAGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAACCTTTTCATTATCCGAAAAATATTCGCCTTCTTGCTTTAAACGAATAGGGCCTGGAGCCGTATCCGCACGGCTGGGAtgt                              >  2:375180/1‑251 (MQ=255)                                                                                                                                                                                                           ccACTGCATACCGGACATATCTACCTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAg                        <  1:417248/251‑1 (MQ=255)                                                                                                                                                                                                                                  ccTTATCCAGCGCGTCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAAGATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATg  <  1:388256/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            GAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATG  >  NC_000913/4627335‑4627809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑