Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,597,626	C→T	A606T (GCG→ACG)	opgB ←	phosphoglycerol transferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,597,626	0	C	T	98.1%	178.6 / NA	54	A606T (GCG→ACG)	opgB	phosphoglycerol transferase I
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (23/29);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGAAACGCAGCGGTGCTGAGTATTCGCTTTCC  >  NC_000913/4597380‑4597861                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            tATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCGTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCGTTGTAACGCGTCAt                                                                                                                                                                                                                                          <  1:285763/250‑1 (MQ=255)    tGCCGTATGCTTTGGCGGTAATCACCAGGTCACATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCg                                                                                                                                                                                                                                      >  2:27734/1‑251 (MQ=255)        gTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTAGGCAGCGGATGCTTGTACTAGATTTTTCCTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAGATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTATAGCGAATATCGTTAACAACAATCTTCACAGTCTCGCCATTGCCTTTGTAACTCGCCATGTCgctg                                                                                                                                                                                                                                   >  2:275907/1‑247 (MQ=255)                 ggCGGTAATCACCAGGGCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATGTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTGTCACCTCTTCTGGGGCACCTGCTACGTTAAAGATGAAACTGTCTGCTTTGTAGCGAATATCGTTATCAACAATCGTCAGCGTATCGACATTGCCTTTGTAACGCGGCATGTCGATGACCGTATCt                                                                                                                                                                                                                          <  2:183577/250‑1 (MQ=255)                       aaTCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATg                                                                                                                                                                                                                   >  1:11490/1‑251 (MQ=255)                        aTCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGc                                                                                                                                                                                                                  >  1:143089/1‑251 (MQ=255)                         tCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGAcc                                                                                                                                                                                                                                >  1:356840/1‑236 (MQ=255)                         tCACCAGGTCAAAGTTCGTCGGCAGCGGGTGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGAcc                                                                                                                                                                                                                                <  2:356840/236‑1 (MQ=255)                          cACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGcc                                                                                                                                                                                                                 <  2:223689/250‑1 (MQ=255)                                       ttcttcGGCAGCGGATGCTTGTACTCGATGTTTGCTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCGTTGTAACGCGTCATGGCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGcc                                                                                                                                                                                                    <  2:216513/250‑1 (MQ=255)                                         cttcGGCAGCGGAAGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGCATCGACCTTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAACGCCGATTTGCCCTGCCAtg                                                                                                                                                                                                 >  1:316660/1‑251 (MQ=255)                                           tcGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACGCCGTACGGGAAATCCCGTTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCTTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCAtg                                                                                                                                                                                                 <  1:417453/249‑1 (MQ=255)                                                  cGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAGTCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGt                                                                                                                                                                                        >  1:102206/1‑251 (MQ=255)                                                                cGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAAc                                                                                                                                                                           <  2:326602/250‑1 (MQ=255)                                                                cGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACAGGCTGAACa                                                                                                                                                                          <  2:225139/251‑1 (MQ=255)                                                                         cTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTg                                                                                                                                                                  >  2:15284/1‑250 (MQ=255)                                                                           tCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATTAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGGCAGTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGa                                                                                                                                                                 <  1:257101/249‑1 (MQ=255)                                                                                   cAGCTGCGCGTTGGACCAGCGGCCCCAAGCATCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCt                                                                                                                                                       <  2:109417/251‑1 (MQ=255)                                                                                                         ccccACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTc                                                                                                                                 <  2:63380/251‑1 (MQ=255)                                                                                                                    cGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTg                                                                                                                      >  2:95944/1‑251 (MQ=255)                                                                                                                          ggAAATCCCGCTAAACTGTTTCACCTTTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTGTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGTCCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGtt                                                                                                                <  1:152334/251‑1 (MQ=255)                                                                                                                                 ccGCTAAGCTGCTTCGCCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCATATCGACCTTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTACATGCCGTTTTGCCCTGCCATGTTGTTTTGCCAACATGCTGAACACTTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCCGTAGGGGGAAAGTGCCAGTTCCGAAGc                                                                                                         >  2:58697/1‑251 (MQ=255)                                                                                                                                      aaaCTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCCGTCGGTGGAAAGTGCCAGTTCCGGCGccc                                                                                                       >  2:326532/1‑248 (MQ=255)                                                                                                                                              tCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGcc                                                                                            >  1:392252/1‑251 (MQ=255)                                                                                                                                                cccTCTTCTGGCGCGCCGGCGACGTTAAAGATGAAACTGTCGGCTGTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGCGCCCAGAGTTGTGCCAt                                                                                          <  2:332846/250‑1 (MQ=255)                                                                                                                                                 ccTCTTCTGGCGCACCGGCGACGTTAAAGATTAAACTGTCGGCTTTGTAGCGAATATCGTGATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACGATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTGCCGGCGCCCAGAGTTGTGCCATc                                                                                         <  2:67342/251‑1 (MQ=255)                                                                                                                                                 ccTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATc                                                                                         <  1:375637/251‑1 (MQ=255)                                                                                                                                                  ctctTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCt                                                                                        >  2:415718/1‑251 (MQ=255)                                                                                                                                                         ggCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAg                                                                                   <  1:414494/249‑1 (MQ=255)                                                                                                                                                           cgcACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAAc                                                                               <  2:335650/251‑1 (MQ=255)                                                                                                                                                                cGGCGACGTTAAAGATTAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTGGTAGCAACGGTCa                                                                          <  1:358392/251‑1 (MQ=255)                                                                                                                                                                      cGTTAAAGATGAATCTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCAGGTCGATTACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAg                                                                    <  1:122741/251‑1 (MQ=255)                                                                                                                                                                             gATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAAt                                                             <  1:374078/251‑1 (MQ=255)                                                                                                                                                                                        cGGCTTTGTAGCGACTATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAAAGCGTCTTGTCTATGACCGTATCTTTCAATGCCGTTTTGCCCTGCCCTGTTGTTTTGTCAACATGCTGCACATTTTGCTGCCCGCCAAGCTCCCCTTGCGATCCACCCCAGTCGGTGGCACGTGCCACTTCCGGAGCCCAGAGTTTTGCCATCTTGTAGCACCGGTCCACCCAGCCGCACTTGTCGCGTgtt                                                  >  1:69092/1‑249 (MQ=255)                                                                                                                                                                                        cGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTgg                                                   <  2:369801/250‑1 (MQ=255)                                                                                                                                                                                          gctgggTAGCGAAGATCGTGATCAACAAGGTTCAGCGTAGAGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTGTTGCCCTGCCATGTTGTTTTGTCAACATGCGGAACAATTTGCGGACCGCCAAGCGGCCCCTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTGGTGGCATCTTGTAGCAACGGTCAACGCAGACGAAAGTGGCGCGTGGAGc                                                <  2:69092/246‑1 (MQ=255)                                                                                                                                                                                                 aGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCg                                         <  2:143089/251‑1 (MQ=255)                                                                                                                                                                                                     aaTATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGc                                                <  2:355376/240‑1 (MQ=255)                                                                                                                                                                                                     aaTATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGc                                                >  1:355376/1‑240 (MQ=255)                                                                                                                                                                                                      atatCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGcc                                      >  1:96378/1‑249 (MQ=255)                                                                                                                                                                                                        atCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGc                                   <  1:149442/250‑1 (MQ=255)                                                                                                                                                                                                           gTTATCACCCATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCCTTAAATGCCGTTTTGCCCTACCATGTTGTTTTGTCAAAATGCTGAACAATTTGCTGACCGCCAAGCTGCACTTGCGAGACACACCAGTCGGTGGTAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAAACCAGCCGAACTTGTAGCGGGGGGCGAAATCGGCCAGCTgg                                >  2:175165/1‑250 (MQ=255)                                                                                                                                                                                                           gTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCCTGTCGCTGACCGTATCTTTAAATGACGTTTTGCCCTGCCCTGTTGTTTTGTCAACATGCTGAACAATTTGCTGACAGCCAAGCTGCCCTTGCGAGCCACACCAGTCGGTGGAAAGAGCCAGTTCCGGCGCCCAGAGTTGTGCCATCTTGTAGCAACGCTCACCCCAGACGACATTGTCGCGTGGTGCGCAATCGGCCAGCTgg                                >  1:267928/1‑250 (MQ=255)                                                                                                                                                                                                             tATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTACATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGCGTTGTGCCCTCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGaa                              >  2:261855/1‑250 (MQ=255)                                                                                                                                                                                                                caacaaTCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGTAGCGAAATCGGCCAGCTGGAAACg                           <  2:81721/250‑1 (MQ=255)                                                                                                                                                                                                                caacaaTCTTCAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGAAACg                           <  1:415718/250‑1 (MQ=255)                                                                                                                                                                                                                          cAGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCGTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCTGAGCCCAGAGTTGTGCCAGCTTGTAGCAACGGTCAACCCAGACTAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGAAACGCAGCGGTGCt                 <  1:261855/250‑1 (MQ=255)                                                                                                                                                                                                                           aGCGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGAAACGCAGCGGTGCTg                >  1:22868/1‑250 (MQ=255)                                                                                                                                                                                                                             cGTATCGACCTTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGAAACGCAGCGGTg                   >  2:177715/1‑245 (MQ=255)                                                                                                                                                                                                                             cGTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGAAACGCAGCGGTg                   <  1:177715/245‑1 (MQ=255)                                                                                                                                                                                                                              gTATCGACATTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTATAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGAAACGCAGCGGTGCTGAgg             >  1:418660/1‑249 (MQ=255)                                                                                                                                                                                                                                   gACATTGCCTTTGTAAAGCGTCATGTCGCCGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGAAACGCAGCGGAGCTGAGTATTCGc       >  2:302556/1‑251 (MQ=255)                                                                                                                                                                                                                                      aTTGCCTTTGTAACGCGTCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGAAACGCAGCGGGGCTGAGTATTCGCttt    >  2:304606/1‑251 (MQ=255)                                                                                                                                                                                                                                         gCCTTTGTAACGCGTCATGTCGATGACCGTAGCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCGTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGAAACGCAGCGGTGCTGAGTAGTCGCTTTcc  <  2:192104/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            TATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTCGGTGGAAAGTGCCAGTTCCGGAGCCCAGAGTTGTGCCATCTTGTAGCAACGGTCAACCCAGACGAAATTGTCGCGTGGAGCGAAATCGGCCAGCTGGAAACGCAGCGGTGCTGAGTATTCGCTTTCC  >  NC_000913/4597380‑4597861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑