Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,595,749	C→T	A35T (GCG→ACG)	lgoR ←	putative DNA‑binding transcriptional regulator LgoR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,595,749	0	C	T	100.0%	92.2 / NA	30	A35T (GCG→ACG)	lgoR	putative DNA‑binding transcriptional regulator LgoR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/16);  total (14/16)

AGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCTGCCATTTTTCATTTTCATTGATATCTAAGGCAACAAAAAGT  >  NC_000913/4595508‑4595976                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    aggtGCAGTTCAGAAAACGTCTCCCCTGGACGCAACTGGCGCTGGGTTATCATCGTGAAAAAAACCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGCATCTGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGAACt                                                                                                                                                                                                                            >  2:37235/4‑251 (MQ=255)   gtGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGGTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGGCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTTCATTTCCGTCATCGCACttt                                                                                                                                                                                                                          <  2:224870/250‑1 (MQ=255)     gCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTg                                                                                                                                                                                                                         <  1:404661/250‑1 (MQ=255)                        cccTGGGCGTAACTGGCGCTGGTTTATCATAGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTCAAGCGGTAGTACAGGCGAACCCGTCCTCATGGTCAGGGTTACGCGCAATAACATAGTCGCTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAggg                                                                                                                                                                                                      >  1:401993/1‑250 (MQ=255)                                   aCTGGCGCTGGATTATCCTAGTGAAAAAAGCCTGCTCAAACTCTTTGTTTTGTTCACTCATTGACGCGGTAGCACAGGCGCAACCGTCATCATGGTCCGGTTTACGCGAAATACCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCCTTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGAACTTTGCGATGGAAGTGGGGAAGGGATATGACCCcgc                                                                                                                                                                                          >  2:255908/1‑251 (MQ=255)                                        cgcTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCat                                                                                                                                                                                     <  1:67170/251‑1 (MQ=255)                                          cTGGTTTATCATCGTGAAAACAGCCTGCTCAAACACTTTGTTTTGTTCAATCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCatat                                                                                                                                                                                   >  2:222214/1‑251 (MQ=255)                                           tGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATatc                                                                                                                                                                                  >  1:88241/1‑251 (MQ=255)                                                       gTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAt                                                                                                                                                                            <  2:414804/245‑1 (MQ=255)                                                       gTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAt                                                                                                                                                                            >  1:414804/1‑245 (MQ=255)                                                            aaaaGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTa                                                                                                                                                                  >  1:219116/1‑250 (MQ=255)                                                                            ccTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTaa                                                                                                                                                 <  2:174477/250‑1 (MQ=255)                                                                                            tCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTc                                                                                                                                 >  2:57947/1‑251 (MQ=255)                                                                                                  aaGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCat                                                                                                                                                                                     <  1:132112/193‑1 (MQ=255)                                                                                                  aaGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCat                                                                                                                                                                                     >  2:132112/1‑193 (MQ=255)                                                                                                     cGGTAGAACAGGCGAAACCGTCATCATGTTCAGGATTACGCGAAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTtcc                                                                                                                        >  1:337784/1‑251 (MQ=255)                                                                                                              aGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGCGAATATGACGCACTGTGGTGCGGCTGCTGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTtgtg                                                                                                                >  2:202698/1‑250 (MQ=255)                                                                                                               ggCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTtgtg                                                                                                                >  1:66218/1‑249 (MQ=255)                                                                                                                           atcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAAcc                                                                                                  <  1:312628/251‑1 (MQ=255)                                                                                                                              atGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCgt                                                                                               >  2:123653/1‑250 (MQ=255)                                                                                                                                                     cATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCgtgtg                                                                        <  2:216199/251‑1 (MQ=255)                                                                                                                                                           cGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACAGTTTTATGCGTGTGTTTTc                                                                   <  1:134056/250‑1 (MQ=255)                                                                                                                                                                        cGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGAtttt                                                     >  1:304152/1‑251 (MQ=255)                                                                                                                                                                           cAAGACGCCGCATTCGGGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTttcc                                                   <  1:136534/250‑1 (MQ=255)                                                                                                                                                                             aGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACAGTTTTATGCGTGTGTTTTCACAAAAAAGATTttccgtt                                                <  2:88241/251‑1 (MQ=255)                                                                                                                                                                                  ccgcATTCGCGTAAGTGGCTGAGAATATGCCGCACTGTGGTGCGGCTGATGTTGTACATTGCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCAGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACGCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGACTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCt                                           <  2:382852/251‑1 (MQ=255)                                                                                                                                                                                  ccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCt                                           <  2:2113/251‑1 (MQ=255)                                                                                                                                                                                                      gagaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTGTCCGTTCCGCTGCCATTTttcattttcatt                        <  1:340922/250‑1 (MQ=255)                                                                                                                                                                                                       agaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAGTTTGTGAACGACTCATATTTTCCTCCTTTTGAGTCCATTAAAGCCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCTGCCATTTTTCATTTTCATTGa                      <  1:202698/251‑1 (MQ=255)                                                                                                                                                                                                                           gCGGCTGATGTTGTACATTTCCGTCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCTGCCATTTTTCATTTTCATTGATATCTAAGGCAACAAAAAGt  <  1:222214/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    AGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCTGCCATTTTTCATTTTCATTGATATCTAAGGCAACAAAAAGT  >  NC_000913/4595508‑4595976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑