Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,043,253	G→A	W280* (TGG→TAG)	srkA →	stress response kinase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,043,253	0	G	A	100.0%	119.3 / NA	35	W280* (TGG→TAG)	srkA	stress response kinase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/17);  total (18/17)

ACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGGCGCAGTATGAAGATGGTAA  >  NC_000913/4043022‑4043494                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  aCGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTAAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCt                                                                                                                                                                                                                                >  2:264879/1‑251 (MQ=255)       ggAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTTGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACCCCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGGTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATccc                                                                                                                                                                                                                          <  2:87389/251‑1 (MQ=255)                   ggCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGaaa                                                                                                                                                                                                              <  2:326249/251‑1 (MQ=255)                      gcgttGGTCCAAGGGTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTTAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAAt                                                                                                                                                                                                           <  1:32381/247‑1 (MQ=255)                           ggTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTccc                                                                                                                                                                                                      >  2:314026/1‑251 (MQ=255)                           ggTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTccc                                                                                                                                                                                                      >  2:373777/1‑251 (MQ=255)                                        ttgATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCggg                                                                                                                                                                                         >  1:203504/1‑251 (MQ=255)                                         tgATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCggg                                                                                                                                                                                         <  2:203504/250‑1 (MQ=255)                                         tgATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAAGTTCCCGTGGTTAACCggg                                                                                                                                                                                         <  1:336288/250‑1 (MQ=255)                                          gATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCgggg                                                                                                                                                                                        >  1:107183/1‑250 (MQ=255)                                                            aaTGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGGTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACa                                                                                                                                                                     <  1:67530/251‑1 (MQ=255)                                                             atggtcacgCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAAATGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTCCCTAGCTAATGCGGCGTTGGGCTGATACCGCGGTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCgcc                                                                                                                                                                      >  2:243276/9‑247 (MQ=255)                                                                        gTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGAt                                                                                                                                                                                   >  1:45981/1‑225 (MQ=255)                                                                        gTTCAGGAGTTGGGTATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAATCTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACTGCTTAAATCGGACTGATTGAGCCTTTACGCGCCATGCGTGTGGTTTAGTATCTTGCCTAGCTAATGCTGCGGTGTGCTGATCCCGCGTTCCCGAAAAATGTCCCGTGGGTAACCAGGGAAGAt                                                                                                                                                                                   <  2:45981/225‑1 (MQ=255)                                                                            aGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTtata                                                                                                                                                     <  1:373777/251‑1 (MQ=255)                                                                                  tgtgGATGTTGCTCAATGGCGATAAAGACGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACAACGCTGAAATCGGCCTGATTGAACCTTTACGCGCCATGGGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCGGCGACAGACGGCGACTTTTATAGacc                                                                                                                                                 >  2:37871/1‑247 (MQ=255)                                                                                     ggATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGATCAGGc                                                                                                                                             <  1:18828/250‑1 (MQ=255)                                                                                               cAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGCAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGCCGGCGCCTTTTATAGAACAGGCAAAAGTTCTAc                                                                                                                                  >  2:285206/1‑251 (MQ=255)                                                                                               cAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCt                                                                                                                                    <  1:163764/249‑1 (MQ=255)                                                                                                                                    acgattattGAAGCTTATGAAGAATTTAGCGAGTTCGACAACTGTGAAATCGGACTGATTGAGCCTTTACGCGCCATGCGTTTGGTTTATTATCATGCCTAGCTGATGCGGCGTTGGTCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGAGTACTGGCGGCGACAGACGGCGACTTTTATAGAACTGGCAAAAATTCTACAAGAGCCCCCTTTGCAATTAACACCTAGGTATTAAtt                                                                                             <  2:232028/248‑2 (MQ=255)                                                                                                                                               aGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGt                                                                                    >  1:303614/1‑249 (MQ=255)                                                                                                                                                 cTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTa                                                                                                <  2:3627/235‑1 (MQ=255)                                                                                                                                                 cTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTa                                                                                                >  1:3627/1‑235 (MQ=255)                                                                                                                                                         gaagtgaGCGAGTTCGACACCGCTTAAATCGGACTGAGTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGGTGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTGTGCAATTAACACCTATGTATTAATCGGATAGAGTAGATCATGaaaa                                                                        <  2:370850/245‑1 (MQ=255)                                                                                                                                                               aGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCATGAAAAAGAttt                                                                  >  1:205687/1‑251 (MQ=255)                                                                                                                                                                   aGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCg                                                                                            >  1:426096/1‑221 (MQ=255)                                                                                                                                                                   aGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGAGTACTGGCTGCGACAGACGGCGACTTGGATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCg                                                                                            <  2:426096/221‑1 (MQ=255)                                                                                                                                                                    gTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCATGAAAAAGATTtggctg                                                             <  2:107183/251‑1 (MQ=255)                                                                                                                                                                                      cGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGtt                                           >  1:8545/1‑251 (MQ=255)                                                                                                                                                                                                               aTGCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGAAGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCCTGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGgcgc                  >  2:277855/1‑251 (MQ=255)                                                                                                                                                                                                                 gCGTTTGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGGCGCAg                <  2:205687/251‑1 (MQ=255)                                                                                                                                                                                                                     ttGGTTTATTATCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGGCGCAGTAt             <  1:91589/250‑1 (MQ=255)                                                                                                                                                                                                                           tattatCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGGCGCAGTATGAAGATg      >  1:49519/1‑251 (MQ=255)                                                                                                                                                                                                                             ttatCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGGCGCAGTATGAAGATggg    >  2:305526/1‑250 (MQ=255)                                                                                                                                                                                                                                tCTTGCCTAGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGGCGCAGTATGAAGATGGTaa  >  1:7009/1‑250 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  ACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATTTCCCGTGGTTAACCGGGGAAGATTACTGGCTGCGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGGCGCAGTATGAAGATGGTAA  >  NC_000913/4043022‑4043494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑