Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,956,003	G→A	D225N (GAT→AAT)	ilvA →	threonine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,956,003	0	G	A	97.8%	277.8 / NA	91	D225N (GAT→AAT)	ilvA	threonine deaminase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (34/55);  total (36/55)
Fisher's exact test for biased strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGGATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCG  >  NC_000913/3955775‑3956249                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        cagGGGTTCACCTGTGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGGTCAAACAACTGATGCCGCAAATCAAAAGGATAGCCGTAGAAG‑CGGGAGAATCCGCATGCCTGAAAGCCGCGCTGGAGGCGGGGCATCCGGGTGATCt                                                                                                                                                                                                                                   >  2:395687/1‑251 (MQ=255)    gggTTCCCCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGcc                                                                                                                                                                                                                                <  1:110707/251‑1 (MQ=255)       ttCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAAGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGGTCAAACAACTTAAGCCGCAAATCACAGTGATCGCCGTAGAAG‑CGGGAAACTCCGCCAGCCTGAAAGCGGCGCCGAATGCGGGTCATCCGGTTGCTCTTCcgcg                                                                                                                                                                                                                             >  1:151834/1‑251 (MQ=255)        tCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCCAAGTGGTCGCCGTAGAAA‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCCTCCGGGTGATCTGCcgcgc                                                                                                                                                                                                                            >  1:386516/1‑251 (MQ=255)          cccggggTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGt                                                                                                                                                                                                                          <  2:382732/247‑1 (MQ=255)           ccTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGGTCAAACAACTGATGCCGCAAATCAAAGGGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCcgcgcgg                                                                                                                                                                                                                          >  1:264017/1‑249 (MQ=255)              gggTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCGGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGATG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCAGCCTGTTGATCTGCCGCGCGTCggg                                                                                                                                                                                                                      <  2:261457/251‑1 (MQ=255)                   gcgccgTGCGACCAGCCGATTGTTATTGCCGGTCTAGGCACTCTGGCTCTGTGAA‑TGCTCCAGTAGGACGCTCATCTCGATCGCGTATTTGGGCAAGGCGTCTGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACAGATGCCGCCAATCAAAGAGATCGCCGTAGAAG‑TGGAAGCCTCCGCCTGCCTGAAAGCAGCGCTTAATGCGGGTCATCCGGTTGATCTGCAGCGCGTAGGGCTAtt                                                                                                                                                                                                                 <  2:349644/250‑1 (MQ=255)                       cgTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGGTGCCGCAAATATAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGGAAGCAGCGCTGAATGCGGGGCATCCGGGTGATCTGCCGCGCGTAGGGCTATTTGCt                                                                                                                                                                                                             >  1:292749/1‑251 (MQ=255)                        gTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑g                                                                                                                                                                                                           <  1:27653/251‑1 (MQ=255)                           cGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGAGATCGCCGGAGAAG‑CGGAAGACTCCGCCTGCCCGAAAGCAGCGCTAAATGAGGGTCATCCGGTTGATCTGGCGCGCGTAGGGCTATTTTCT‑Ga                                                                                                                                                                                                          >  1:388085/1‑249 (MQ=255)                             aCCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCAGAACAAAAAAATGAAGGCGCAAATCCAAGGGATCGCCGGAGAAG‑CGGAAAACACCGCCTGCCTGAAAGAAGCGCTGAAAGCGGGGAATCCGGTTGATCTGCCGGGCGGCGGGGTATTTGGT‑GAAggg                                                                                                                                                                                                      >  1:307629/1‑250 (MQ=255)                               cATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGt                                                                                                                                                                                                    <  1:314913/251‑1 (MQ=255)                                  ccGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAg                                                                                                                                                                                                  <  1:399074/250‑1 (MQ=255)                                  ccGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGc                                                                                                                                                                                                 <  1:153957/251‑1 (MQ=255)                                     agggTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCGCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGGTGTCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGGGAGCGCCGTAGAAG‑CGGAATACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGGTCTTCCGCGCGTGGGGCTATTTGCT‑GAAGGCGTAGCGGt                                                                                                                                                                                              <  2:59459/249‑1 (MQ=255)                                       ggtggtTGCCGGGCAAGGCACGCTGTCGCTG‑GAACTGCTCCAGCAGGACGCCTATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCGGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCTCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCGGAAAGCAGCGCTGAATGCGGTTCAGCCGGTTGATCTGCCGCGCGGAGAGCTATTTGTT‑GAAGGCGTGGCGGTaa                                                                                                                                                                                            <  2:182639/251‑1 (MQ=255)                                       ggTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGGTGATCAAACAAATGATGCCGCAAATAAAAGGGATCGCCGGAGAAG‑CGGAAGACTCCGCCTGTCTGTAAGCAGCGCTGAATGCGGGTCATCCGGGTGATCTGCCGCGCGTAGGGCTATTTTCT‑GAAGGAGTAGCGGTa                                                                                                                                                                                             >  2:373205/1‑250 (MQ=255)                                       ggTGATTGCCGGGAAAGGCACGCTGGCGCTG‑GATCTGCTACAGCAGGACGCCCATCTCGACCGCGTATTTGGGCCAGTCGGCGGTGGCGGTCTGTGTGCTGGCGTGGCGGTGCTGATCAAACAACCGATGCCGCAAATCAAAGTGATCGCAGTAGAAG‑CGGAAGGCTCCGCGTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTaa                                                                                                                                                                                            <  1:385888/251‑1 (MQ=255)                                         tGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGGGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTGGGAGACGGA‑GACTCCGCCTGCCCGGAAACAGCGCTGAATGCGGGTCATCCGGTTGGTATGCCGCGCGTAGGGCTATTTGCTTGAAG‑CGGAGCGGTaaa                                                                                                                                                                                           >  1:56908/1‑250 (MQ=255)                                          gATTGCCGGGCAAGGCACGCTGGCGCGG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTAGTTGCT‑GAAGGCGTAGCGGTAAAAc                                                                                                                                                                                         <  2:343319/251‑1 (MQ=255)                                               ccGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATc                                                                                                                                                                                    <  1:43353/251‑1 (MQ=255)                                                   gCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACCTAAGGCGCAAATCAAAGGGATCGCCGGAGGAG‑CGGTAGACTCCCCCTGACTGAACACAGCGCGGAATTCGGGGCATCCGGGTGGTCTGCCGCGCGTCTGGGTATTTGCT‑GAAGGGGGAGCGGGTAAACGCCTCgggg                                                                                                                                                                                >  2:216852/1‑249 (MQ=255)                                                     aaGGCACGCTTGCGATG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAAGCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGATGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑TAAGGCGTAGCGTTAAAACGCATCGGTGAc                                                                                                                                                                              <  1:317442/251‑1 (MQ=255)                                                     aaGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCCAATCCAAGTGATCGCCCTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCCGCGCTGAATGCCGGGCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGGAACGGGAAAACGCATCgggggc                                                                                                                                                                              >  1:258231/1‑247 (MQ=255)                                                          aCGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGTGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAAc                                                                                                                                                                         <  1:337901/251‑1 (MQ=255)                                                              tggcgctg‑gAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGAGTGGCGGTGCTGATCAAACACCTGCTGGCGCACATCAAAGTGATCGCCGGAGAAG‑CGGAAGACTCCCCCTGCCTGAAAGCAGCGCTGGATGCGGGTCATCCGGTTGATTTGCCGCGCGAAGGGCTCTTTGCT‑GCAGGCGTAGCGGGAAAACGGATCGGTGACGGAACCTTc                                                                                                                                                                     >  2:425503/1‑251 (MQ=255)                                                                gcgctg‑gAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTTATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTAGTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAATCCTTCCg                                                                                                                                                                   <  2:270401/251‑1 (MQ=255)                                                                        aaCTGCTCCAGCAGGCCGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGAGGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGTCTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACACATCGGTGACGAAACCTTCCGTTTATGc                                                                                                                                                            <  2:99868/251‑1 (MQ=255)                                                                         aCTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGGTCAAACAACTGATGCCGCCAATCAAAGGGATCGCCGTAGAAG‑CGGCAGACTCCGCCTGCCTGAAAGGAGGGCTGAATGAGGGGCATCCGGGTGATCTGCCGCGCGAAGGGGTATTTGGT‑GAAAGCGGAGCGGGAAAACGAAACGGTGACGAAACCTTCCGGTTATggc                                                                                                                                                           >  1:280415/1‑249 (MQ=255)                                                                         aCTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGcc                                                                                                                                                           <  2:58709/251‑1 (MQ=255)                                                                           tGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGCTCAAACAACCGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGGAGCGGGAAAACGCATCGGTGACGAAACCTTCCGTTTATGcc                                                                                                                                                           >  1:410946/1‑249 (MQ=255)                                                                               ccAGCAGGCCGCCCATCTCGACCGCGTATGTGGGCCAGTCGGCGGCGGCGGTCTTGCTGCTGGCGTTGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTTATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTTCCGCGCGTAGGGCTATTTGCT‑GAAGTCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGt                                                                                                                                                     <  2:307629/251‑1 (MQ=255)                                                                                 cgcagGACGCCCATCTCGACCGCGTATTGGGGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTAt                                                                                                                                                   <  2:172833/250‑1 (MQ=255)                                                                                 agcagGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCCAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGGTGATCTGCCGCGCGTAGGGCGATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAggat                                                                                                                                                   >  2:215583/1‑248 (MQ=255)                                                                                    agGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGGGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGCATAtctc                                                                                                                                                >  1:353445/1‑251 (MQ=255)                                                                                     ggACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTTCTGATCCAACAACTGATGCCCCAAATCCAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCCCGCGTAGGGCTATTTGGT‑GAAGGCGTTGCGGTAAAACGCAACGGGGACGAAACCTTCCGTTTATGCCAGGAggataccg                                                                                                                                               >  1:371311/1‑244 (MQ=255)                                                                                         gCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCCAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAAAGCGGGGCATCCGGGTTATCTGCCGCGCGGAGGGCTCTTTGCT‑GGAGGCGGACCGGTAAAACGCACCAGGGACGAAACCTTCCCGTTATGCCAGGAGGATCTcgtcgc                                                                                                                                           >  2:328855/1‑247 (MQ=255)                                                                                            cATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGTCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGCcat                                                                                                                                        <  2:326982/251‑1 (MQ=255)                                                                                                 cGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGTAGTATCTCGACGAcatca                                                                                                                                      <  2:392755/248‑1 (MQ=255)                                                                                                 cGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAAAAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAAACTCCGCCTGCATGAAAGAAGCGCTGAATGCGGGTCATACGGGTGAACTGCCGGGGGTAGGGCTATTTGCT‑GAAGGCGGAGCGGTAAAACGCATCGGGGAAGAAACCTTCCGGTTTTGCCAGGAGTATTTTGACGAcataata                                                                                                                                    >  1:84899/1‑246 (MQ=255)                                                                                                   aCCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCAcc                                                                                                                                 <  1:106332/251‑1 (MQ=255)                                                                                                    ccGCGTAGTTGTGCCAGGCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCCCCg                                                                                                                                <  2:334560/251‑1 (MQ=255)                                                                                                             tgtgCCAGGCGGCGGCGGCGGTATGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgc                                                                                                                        <  2:132850/250‑1 (MQ=255)                                                                                                             ggggcaaGTCGGCGGCGGCGGGCTGTCTGCTGGCGTGGCGGTGCTGATCAAACAACTTATGCCGCAAATCAAAGTGAGCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGTTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgc                                                                                                                        <  1:214444/244‑1 (MQ=255)                                                                                                                gCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgcgat                                                                                                                     <  1:431472/250‑1 (MQ=255)                                                                                                                  cAGTCGGCGGCGTCGGGCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgcgatgcg                                                                                                                  <  1:55007/251‑1 (MQ=255)                                                                                                                       ggcggcggcggTCTGGCTGCTGGCGTGGCGGTGCCGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCCCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑TAAGGCGTAGGGGTAAAACGCCTAGGTGACGAAACCTTCCGTTTATGCCAGGCGAATCTCGACGACCTCATCACCGTCGATAGCGATGCGATCtg                                                                                                             >  1:15725/1‑251 (MQ=255)                                                                                                                          ggcggcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAAAAACTGATGCCGCAAATCAAAGGGATCGCCGTAGAAG‑CGGGAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGGGATTTGGT‑GGAGGCGGAGAGGGAAAACGCATCGGGGACGAAAACTTCCGTTTATGCCAGGGGGAACTCGGCGACATCATCACCGCCGATAGCGATGCGCTCtgtgg                                                                                                          >  2:344116/1‑250 (MQ=255)                                                                                                                                ggTCGGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCGGCAAATCAAAGTGATCGCCGGAGAAT‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCAGCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAGCGCATCGTTGACGAAACCTTCCGTGTATGCCAGGAGTATCTCGACGACATCAGCACCGTCGATAGCGATTCGATCTATGCTGCGAt                                                                                                    <  2:409032/251‑1 (MQ=255)                                                                                                                                          ctgGCGTGGCGGTGCTGATCAAAAAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAGAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACCTCATCACCGCCGATAGCGCTGCGATCTGTGCGGCGATGAAGGAttt                                                                                           >  1:104400/1‑250 (MQ=255)                                                                                                                                              cgtggcgGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCg                                                                                      <  2:353445/251‑1 (MQ=255)                                                                                                                                                    ggTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCTCCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGAtg                                                                                <  2:383722/251‑1 (MQ=255)                                                                                                                                                    ggTGCTGATCAAACAACGGATGCCGCAAATCAAAGTGCTCGCCGTAGAAG‑CGGCAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGCTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGAtg                                                                                >  2:420184/1‑251 (MQ=255)                                                                                                                                                       gCTGATCAAACAACTGATGCCGCAAATAAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCGGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTgc                                                                             <  2:239535/251‑1 (MQ=255)                                                                                                                                                         tGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGCAGATGTgcgc                                                                           >  2:322240/1‑251 (MQ=255)                                                                                                                                                                      gATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAAc                                                              >  2:244207/1‑251 (MQ=255)                                                                                                                                                                       aTGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAACGCAGCGATGAATGCGGGTCATCCGGTTGATCTGCCGCGAGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGAGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAAcc                                                             >  2:225081/1‑251 (MQ=255)                                                                                                                                                                         gCCGCAAATCAAAGTGGTCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCct                                                           <  2:367489/251‑1 (MQ=255)                                                                                                                                                                           cGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCctct                                                         <  1:357706/251‑1 (MQ=255)                                                                                                                                                                            gCAAATCAAAGTGATCGCCGTAGAAG‑CGGCAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGGGGCGGCACCCTCTg                                                        >  2:372716/1‑251 (MQ=255)                                                                                                                                                                             cAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTgg                                                       <  2:264017/251‑1 (MQ=255)                                                                                                                                                                             cAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTgg                                                       >  1:356979/1‑251 (MQ=255)                                                                                                                                                                              aaaTCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGATCCCTCTGgc                                                      <  2:386516/251‑1 (MQ=255)                                                                                                                                                                                      gggATCGCCGTAGAAG‑CGGAAGACGCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCGCTGGCgcgctggc                                              <  2:242856/249‑1 (MQ=255)                                                                                                                                                                                      gTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgctggc                                              <  1:373073/251‑1 (MQ=255)                                                                                                                                                                                       tGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgctggcg                                             >  1:168404/1‑251 (MQ=255)                                                                                                                                                                                         aTCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgctggcgct                                           <  1:91461/251‑1 (MQ=255)                                                                                                                                                                                          gcGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATGTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgctggcgctg                                          <  1:412225/250‑1 (MQ=255)                                                                                                                                                                                           cGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTGCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgctggcgctg                                          <  1:425503/250‑1 (MQ=255)                                                                                                                                                                                            gCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCgctggcgctggc                                        <  2:124105/251‑1 (MQ=255)                                                                                                                                                                                            gCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCgctggcgctggc                                        <  1:325051/251‑1 (MQ=255)                                                                                                                                                                                               gTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCggg                                     <  1:344116/251‑1 (MQ=255)                                                                                                                                                                                                 aGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGa                                    <  2:366253/250‑1 (MQ=255)                                                                                                                                                                                                  gAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAAt                                  <  1:215583/251‑1 (MQ=255)                                                                                                                                                                                                  gAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAAt                                  >  1:130180/1‑251 (MQ=255)                                                                                                                                                                                                  gAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTGGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTGATGCCAGGAGTATCTCGACGACAGCATCGCCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATTTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAAt                                  <  1:180792/251‑1 (MQ=255)                                                                                                                                                                                                   aaG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGGCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCTGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATg                                 <  1:194204/251‑1 (MQ=255)                                                                                                                                                                                                   aaG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATg                                 <  1:167793/251‑1 (MQ=255)                                                                                                                                                                                                       cGGAAGACCGCGCATGCCTGAAGGCAGCGCAGAATGCGGGTCATCCGGTTGCTCTGGCGCGCGTAGGGCTAGTTGCT‑GAAGGCGTAGCGGTAAAACGCAGCGGTGACTAAGCCTGCCGTTTATGCCTGGAGTATCTCGACGACATCATCGCCGTCGATAGCGAGGCGTTCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGGGGTGGCGGAACCCCCTGGCGCGCTGGCGCTGGCGGGAATGaaa                              <  1:169834/251‑1 (MQ=255)                                                                                                                                                                                                       cGGAAGAATCTGCCTGCCTGAATGCAGGGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGAGTAGAGGTAAATCGCATCGGTGACGACACCTTCCGATTATGCCAGGAGTATCTCGACGACATCAACACCGTCGATAGCGATGCGATCTGTGCGGCGATGAGGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGAGCTGGCGGGAATGaa                               >  2:181838/1‑250 (MQ=255)                                                                                                                                                                                                        ggAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGaaaa                             <  1:395687/251‑1 (MQ=255)                                                                                                                                                                                                         gAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGaaaaa                            <  1:134641/251‑1 (MQ=255)                                                                                                                                                                                                          aaGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAACCCTTCCGTTTATGCCAGGAGAATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGGGCTGCCGCTGGCGGGAATGaaaaa                            >  2:143722/1‑250 (MQ=255)                                                                                                                                                                                                            gacgccGCCTGCCTGAAAGCAGCGCGGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTTCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCTGGAATGAAAAAata                         <  2:292749/247‑1 (MQ=255)                                                                                                                                                                                                                       actgAAAGCAGCGCTGAATGCGGGTCATACGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAGGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCCGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGACGATGCAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCTGGAATGAACAAATATATCGCCCTGc              >  2:263862/2‑251 (MQ=255)                                                                                                                                                                                                                          gAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATATCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGTTGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAACAATATATCGCCCTgaaa            >  1:433174/1‑247 (MQ=255)                                                                                                                                                                                                                            aaGCAGTGCTGAATGCGGGGCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATAGATCGCCCTGCacaac         <  2:56908/251‑1 (MQ=255)                                                                                                                                                                                                                            aaGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAACACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTCTCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCacaac         >  1:427501/1‑251 (MQ=255)                                                                                                                                                                                                                              gcagcGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACAt       <  2:241916/251‑1 (MQ=255)                                                                                                                                                                                                                                   gcTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGCAAAAATAAATCGCCCTGCACCACATTcgcg  >  2:300067/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        CAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTG‑GAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAG‑CGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGGATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCT‑GAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCG  >  NC_000913/3955775‑3956249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑