Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,890,364	C→T	A43A (GCC→GCT)	tnaB →	tryptophan:H(+) symporter TnaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,890,364	0	C	T	100.0%	97.7 / NA	30	A43A (GCC→GCT)	tnaB	tryptophan:H(+) symporter TnaB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

GCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCCTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTAATCGGTAACACCTGGAACATTATCAGCGGTATTACCGTTGCCTTCGTTCTCTATATCCTCACTTATGCCTATATCTCTGCTAATGGTGCGATCATTAGTGAAACGATATCAATGAATTT  >  NC_000913/3890125‑3890594                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                       gCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTAt                                                                                                                                                                                                                             <  1:123129/251‑1 (MQ=255)                gTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGAACTCTGCATTTTGGGGTGTTATGGTTATCGCAGGTACAGTAATTGGAGGAGGTATATTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCATTATCATTGCCTGGTTTTc                                                                                                                                                                                                              >  1:402379/1‑251 (MQ=255)                     attaatACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGc                                                                                                                                                                                                         >  1:247040/1‑251 (MQ=255)                        aatACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGCTCAAGCTGAAAAATAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGATTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTc                                                                                                                                                                                                      >  2:34795/1‑251 (MQ=255)                              acAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTc                                                                                                                                                                                                  <  2:67420/249‑1 (MQ=255)                                     ggCTATAAGGATGTTAGACACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGgttatt                                                                                                                                                                                         >  2:15278/1‑251 (MQ=255)                                         ataAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGAGCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCGTATCATTGCCTGGTTTTCAATGCTTCATTCCGGgtgattgt                                                                                                                                                                                       <  2:392672/249‑1 (MQ=255)                                             ggATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGgttattgttatt                                                                                                                                                                                   >  1:89898/1‑249 (MQ=255)                                                           ctctTACACTACAACCTCAAGAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTTCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTAGTGTTATTAGAAGCAAACTTaa                                                                                                                                                                     <  1:236062/249‑1 (MQ=255)                                                                aCCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATcc                                                                                                                                                              <  1:117400/251‑1 (MQ=255)                                                                     aCATCCTCAATAACAAAAATAGCCTTCCTCTAATGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTACTTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCACATTTAAATTATCCCGTCg                                                                                                                                                         >  1:282921/1‑251 (MQ=255)                                                                     aCATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTc                                                                                                                                                          <  1:367368/250‑1 (MQ=255)                                                                         ccTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTc                                                                                                                                                     <  2:45743/251‑1 (MQ=255)                                                                            cAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTTCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCa                                                                                                                                                   <  1:282716/250‑1 (MQ=255)                                                                                              tCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCg                                                                                                                                                            <  2:47592/223‑1 (MQ=255)                                                                                              tCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCg                                                                                                                                                            >  1:47592/1‑223 (MQ=255)                                                                                              tCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCAcc                                                                                                                                  <  1:34951/249‑1 (MQ=255)                                                                                               ccTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCaaa                                                                                                                               <  1:122057/251‑1 (MQ=255)                                                                                                         tGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTAATCg                                                                                                                     >  1:345659/1‑251 (MQ=255)                                                                                                                atGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATcc                                                                                                                                                              >  1:168669/1‑203 (MQ=255)                                                                                                                atGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATcc                                                                                                                                                              <  2:168669/203‑1 (MQ=255)                                                                                                                              tGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTAATCGGTAACACCTGGAACATTATc                                                                                                 >  2:320046/1‑250 (MQ=255)                                                                                                                                                     ggggAGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGAGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCAGTCGGCTCCAGTTTTAACACCATCACCAAAGCTTTAATCGGTAACACCTGGAACTTTATCAGCGGTATTACCGTTGCCTTCGtt                                                                         >  1:10158/1‑251 (MQ=255)                                                                                                                                                        gtgtTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTAATCGGTAACACCTGGAACATTATCAGCGGTATGACCGTTGCCTTCGTtctc                                                                      <  2:393868/251‑1 (MQ=255)                                                                                                                                                                        aGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTAATCGGTAACACCTGGAACATTATCAGCGGTATTACCGTTGCCTTCGTTCTCTATATCCTCACTTATg                                                      >  1:69179/1‑251 (MQ=255)                                                                                                                                                                                                      tttACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTAAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTAATCGGTAACACCTGGAACATTATCAGCGGAATTACCGTTCCCTTCGCTCTCTATCTCCTCACTTATCCCTCTAtccctcctacccgcgccatcattc                        >  1:30596/1‑229 (MQ=255)                                                                                                                                                                                                                     tGCCGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTAATCGGTAACACCTGGAACATTATCAGCGGTATTACCGTTGCCTTCGTTCTCTATATCCTCACTTATGCCTATATCTCTGCTAATGGTGCGATCATTAGTGAAACGata             <  1:320046/247‑1 (MQ=255)                                                                                                                                                                                                                       ccGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTAATCGGTAACACCTGGAACATTATCAGCGGTATTACCGTTGCCTTCGTTCTCTATATCCTCTCTTATGCCTATATCTCTGCTa                                       <  2:400878/219‑1 (MQ=255)                                                                                                                                                                                                                       ccGGTGCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTAATCGGTAACACCTGGAACATTATCAGCGGTATTACCGTTGCCTTCGTTCTCTATATCCTCACTTATGCCTATATCTCTGCTa                                       >  1:400878/1‑219 (MQ=255)                                                                                                                                                                                                                            gCCTGGTTTTTCTGGGGTGCTTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTAATCGGTAACACCTGGAACATTATCAGCGGTATTACCGTTGCCTTCGTTCTCTATATCCTCACTTATGCCTATATCTCTGCTAATGGTGCGATCATTAGTGAAACGATATCAATGAAttt  >  1:34030/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                       GCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAAAGGTGGCATCATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTATGTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCCTTTATCCTTATCATTGCCTGGTTTTCAATGCTTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTAATCGGTAACACCTGGAACATTATCAGCGGTATTACCGTTGCCTTCGTTCTCTATATCCTCACTTATGCCTATATCTCTGCTAATGGTGCGATCATTAGTGAAACGATATCAATGAATTT  >  NC_000913/3890125‑3890594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑