Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,820,223	T→C	A316A (GCA→GCG)	ligB ←	DNA ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,820,223	0	T	C	97.8%	145.5 / ‑4.3	45	A316A (GCA→GCG)	ligB	DNA ligase B
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (22/22);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

AGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCT  >  NC_000913/3819976‑3820460                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              aGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATTATCAAAGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCAAGCGCCTGACGAAACCAATATTCACCCGCTGGCCTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCACCCACCGCTATTTTACAGCTCTTACCCACCTCAAACTGAATCGcc                                                                                                                                                                                                                                            >  1:284662/1‑251 (MQ=255)  gTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATTATCAATGCGAGGAATAACCTGACCGGCAAGGCTGACGAGAATCTGATCCACAGGCGCAATATCCCACTCCTGCCAGCGCCTGAAGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAAGTGCGAGTGCCGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCt                                                                                                                                                                                                                                           >  1:270313/1‑251 (MQ=255)        gCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATTATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTAACtt                                                                                                                                                                                                                                     >  1:40964/1‑251 (MQ=255)                                 gTACGTTCTGCACCGCGCCACACCACATTATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGat                                                                                                                                                                                                             <  2:364422/250‑1 (MQ=255)                                          gCACCGCGCCACACCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGAAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAgg                                                                                                                                                                                                    <  2:120145/250‑1 (MQ=255)                                                         acaTTATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGc                                                                                                                                                                                    >  1:403223/1‑251 (MQ=255)                                                                             aCCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGc                                                                                                                                                                >  1:330884/1‑251 (MQ=255)                                                                                          aGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGctc                                                                                                                                                   <  1:82899/251‑1 (MQ=255)                                                                                                aCGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGccacc                                                                                                                                                                                               >  2:356854/1‑201 (MQ=255)                                                                                                aCGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGccacc                                                                                                                                                                                               <  1:356854/201‑1 (MQ=255)                                                                                                aCGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGCTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCAGGCTCTTTcgc                                                                                                                                             >  1:41490/1‑251 (MQ=255)                                                                                                  gagaATCTGAACCCCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTGTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTcgccg                                                                                                                                           <  2:137344/251‑1 (MQ=255)                                                                                                           aTCACCAGGCGCAATATACCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGAAGCCAATGGCGGGATTCTGGCTCTTTCCCCGCCCGtcca                                                                                                                                   >  1:299297/1‑247 (MQ=255)                                                                                                            ccACCAGGCGCAATACCCCCCCCCTGCCAGCGCCTGACGGAACCAATATTCCCCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTTAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAAc                                                                                                                                 <  2:336825/250‑1 (MQ=255)                                                                                                             cACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCAACCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAGACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTACCCGCCCGTACAACt                                                                                                                                >  1:387179/1‑251 (MQ=255)                                                                                                                            cccACTCCTGCCAGCGCCTGACGGAACCAAAATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACCACTACGCCATCGGGGACg                                                                                                                 >  1:97502/1‑251 (MQ=255)                                                                                                                               aCTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGGTTCTGGCTCTTTCGCCGCCCGTACAACTACGCCATCGGTGACGa                                                                                                                >  2:3252/1‑249 (MQ=255)                                                                                                                                 cccTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAATCTGAACCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAggg                                                                                                            <  2:414568/250‑1 (MQ=255)                                                                                                                                   cTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGGTTCTGGCTCTTTCGCCGCCCGTACAACTACGCCATCGGTGACGGAGGGt                                                                                                           >  2:202093/1‑250 (MQ=255)                                                                                                                                          cgcCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGc                                                                                                   <  1:399682/251‑1 (MQ=255)                                                                                                                                              tGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGCGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTTGCCGCTCGTACAAATACGCCATCGGTGACGACGGGGAATTTCGCtttt                                                                                               >  2:171569/1‑250 (MQ=255)                                                                                                                                                 cGGACCCAGTATTCCCCCGCTGGACTTTTTTATCTTCCAGCATGACAGGTGCGAGTAACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCGCTTCGGTAACCTGAGCTACAGGTTGATATTTCCAGGCCCCCAGCCACTCTGCCGGGCCCTGTATCCAATGGCGGGATTCTGGCTCTTTCGTCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTccac                                                                                            <  1:204608/251‑1 (MQ=255)                                                                                                                                                     aCCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCCTCGGTGACGAAGGGTAATTCCGCTTTCCACCACt                                                                                        >  1:235817/1‑251 (MQ=255)                                                                                                                                                              cACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGGAGGGTAATTCCGCTTTCCACCACTCATTGCGt                                                                                >  1:80477/1‑250 (MQ=255)                                                                                                                                                                cccGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATg                                                                                                                                                                  >  2:83318/1‑166 (MQ=255)                                                                                                                                                                cccGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATg                                                                                                                                                                  <  1:83318/166‑1 (MQ=255)                                                                                                                                                                  cGCTGGACTTTTTTATCATCCAGCATGCCAGGTGCGAGTGACTCAACCACCTATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTAcgcg                                                                           <  2:114908/251‑1 (MQ=255)                                                                                                                                                                  cGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCGTTCCACCACTCATTGCGTAcgcg                                                                           <  1:123927/251‑1 (MQ=255)                                                                                                                                                                          ttttttATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTTGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGGAATTTCGCTTTCCCCCCCTCATTTCGTAccggggccactt                                                                   >  2:400500/1‑240 (MQ=255)                                                                                                                                                                           tttttATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACtt                                                                   <  1:228991/250‑1 (MQ=255)                                                                                                                                                                                  catcCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCCACTTCATCAGc                                                            >  1:316632/1‑250 (MQ=255)                                                                                                                                                                                       cgCATGACAGGTGCGAGTGACGCAACCTCCGATATTTTACCGCTCTTACCAACCGCAAACTGAATCGCCTTCACTGCGGCAACCTGAGCTACAGGTTGATAGTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGGGACGAAGGGTAAGTCCGCTTTCCAACACTCATTGCGTACGCGCGTAACTTCATCAGCAttttt                                                      <  2:248079/250‑1 (MQ=255)                                                                                                                                                                                         cATGACAGGTGCGAGTGACGCAACCCCCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCa                                                    <  2:97502/251‑1 (MQ=255)                                                                                                                                                                                               aGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGGAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATTAGCATTTTTCACCGCAc                                              >  1:88936/1‑251 (MQ=255)                                                                                                                                                                                                 gTGCGAGTGACGCCACCTCCTATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCTGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACgg                                            <  2:278616/251‑1 (MQ=255)                                                                                                                                                                                                     gAGTGACGCCACCTCCGATATTGTACCGCTCTTACCCGCCGCAACCTGCATCCCCTTCACTTCGGCTACCTGAGCTACAGGTTGATATTTCCAGGCCGCCAGCCCCGCTTCCTGTCCCGGTAGCCAATGGCGGGATTCTGGCTCTTGCGCCGCTCGTACAACTACGCCATCGGTGACGAAGTGTAATTCCGCTTTCCCCCACTCATTGCTTACGCGCGCAACTTCATCAGTCTTTTTCCCCGCACGGGtat                                        <  2:419968/251‑1 (MQ=255)                                                                                                                                                                                                            gCAACAACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCCCCGCACGGGTATACGTCTg                                 >  2:183929/1‑251 (MQ=255)                                                                                                                                                                                                             cAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAAGTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGc                                <  1:171569/251‑1 (MQ=255)                                                                                                                                                                                                               cccaccGATATTTTCCCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAgg                                                                                                             <  2:331937/171‑1 (MQ=255)                                                                                                                                                                                                               accaccGATATTTTACCGCTCTTACCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAgg                                                                                                             >  1:331937/1‑172 (MQ=255)                                                                                                                                                                                                                    cGATTTTTTCCCGCTCTTCCCCACCGCAAACTGAATCGCCTTCACTTCGGCAACCGGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTagag                         <  2:80477/251‑1 (MQ=255)                                                                                                                                                                                                                                 ccctTACCCCCCGCAAGCGTAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATTTTTCCAGGCCACCAGCCCCTCTGCCCGGCCCGGTAGCCAATGGCGGGAGTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCTGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCTACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGt            <  2:403223/249‑1 (MQ=255)                                                                                                                                                                                                                                       cacACCGCAACCTGAATCGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCCCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGGAGGGGAATTCCGCTTTCCCCCACCCATTGCGTACGCGCGCAACTTAATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAAACTGCGGTAggc        >  2:57395/3‑247 (MQ=255)                                                                                                                                                                                                                                         caccgacaaCTGAATCGCCTTCCCTTCGGCAACCTGAGCTACAGGTTGATATTCCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGct    <  2:327961/244‑1 (MQ=255)                                                                                                                                                                                                                                           ccGCAAACTGAATCGCCTTCACTTCGGCACCCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGctct  <  1:303889/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              AGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATACGTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCT  >  NC_000913/3819976‑3820460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑