Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,819,185	G→C	G104G (GGG→GGC)	yicG →	PF03458 family inner membrane protein YicG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,819,185	0	G	C	100.0%	142.3 / NA	44	G104G (GGG→GGC)	yicG	PF03458 family inner membrane protein YicG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (23/21);  total (23/21)

CGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTCGGACTGGTGGTCTTTTCTATCATCGGGGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTG  >  NC_000913/3818946‑3819423                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                cgccgcatgattACATTTGGCGGAATTATTAGTGCGACCGCCACCGCAATTGGCGGAGGGCCAGTGCTCGATATTCTGCTGGGCCACGATCCGCTCGGCTGGGTCAAACCCCCGGAATATGTGATTATCGTCGGTACCGCCGCAGTACTCACCACTAGCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCTCAGGTTGc                                                                                                                                                                                                                                      <  2:150038/240‑1 (MQ=255)    cgcATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACt                                                                                                                                                                                                                                   >  1:423010/1‑251 (MQ=255)       aTGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTgg                                                                                                                                                                                                                                 >  1:10453/1‑250 (MQ=255)                          ttattattGCGACCGCCACCGCCATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAatt                                                                                                                                                                                                             >  2:339145/1‑251 (MQ=255)                            gttattGCGACAGCCCCCCCAATTGGGGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTTCCCGCGCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTCCCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGGTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAattat                                                                                                                                                                                                           <  2:238330/250‑1 (MQ=255)                              tattGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTg                                                                                                                                                                                                         >  2:311886/1‑251 (MQ=255)                                  gcgcccGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCGGGGCCCCTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGt                                                                                                                                                                                                     <  2:243762/247‑1 (MQ=255)                                   cGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGtt                                                                                                                                                                                                    <  1:311886/251‑1 (MQ=255)                                    gACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTg                                                                                                                                                                                                   >  2:340074/1‑251 (MQ=255)                                    gACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTg                                                                                                                                                                                                   >  1:17052/1‑251 (MQ=255)                                      ccGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCCCAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTc                                                                                                                                                                                                 >  2:249209/1‑251 (MQ=255)                                                               aGTGCGCGATATTCTGCTGGGCCACTATCAGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCTGCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTg                                                                                                                                                                        <  2:109437/251‑1 (MQ=255)                                                                           tCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTgc                                                                                                                                                            >  1:367691/1‑251 (MQ=255)                                                                           tCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTg                                                                                                                                                             <  1:52327/250‑1 (MQ=255)                                                                                tgGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGGTTGGTGGCGTTTTGGGCGat                                                                                                                                                       >  1:363048/1‑251 (MQ=255)                                                                                 gggCCACTATCCGCTCGGCTGGGTCAAACCCCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGt                                                                                                                                                                 <  1:342581/240‑1 (MQ=255)                                                                                 gggCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGtagaaggggagg                                                                                                                                                      >  2:342581/1‑240 (MQ=255)                                                                                     cccTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGtt                                                                                                                                                  <  1:111470/249‑1 (MQ=255)                                                                                         aTCCGCTCGGCTGTGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCt                                                                                                                                                <  1:178820/249‑1 (MQ=255)                                                                                           ccGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTaa                                                                                                                                            <  1:324878/251‑1 (MQ=255)                                                                                                          aaaCACCCGGAATATGTGGTTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGATCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCACTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGAGTGTTTGGCGCAGGTTTGCGCGATATGTTCTGTAAACGTAC‑CCCACTggg                                                                                                                            >  2:172792/1‑250 (MQ=255)                                                                                                                       atGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGGGGTTGGCGGCGTTTTGCGGGATATGTTTTGGAAACGTAT‑CCCAATGGTATTCCAGAAag                                                                                                                 >  1:1248/1‑249 (MQ=255)                                                                                                                         gtgATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTGTTTCAGAAAGAGtt                                                                                                             >  1:320506/1‑251 (MQ=255)                                                                                                                              tATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTTGCGGCGTTTTGCGCGATATGTTCTGTAACCGTAT‑CCCCCTGGTATTCCAGAAAGAGTTTTATg                                                                                                        >  1:393199/1‑251 (MQ=255)                                                                                                                                  gtcgCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCgg                                                                                                    >  1:129731/1‑251 (MQ=255)                                                                                                                                        cccgccgCAGTACTCACCACTATCGTTGCTCCGGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCTCTTGGACTGGTGGTCTTTTCTATCATCGGCGCTCAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCTGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCTCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGtctc                                                                                              <  1:141833/250‑1 (MQ=255)                                                                                                                                         ccgccgCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCa                                                                                             <  1:386584/251‑1 (MQ=255)                                                                                                                                          cgccgcAGTACTCCCCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCGCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCCCAGGTTGACCTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGGAACCACCGGCGGGTTTGGCGTCGTTTTGGGCGATATGGTCTGGAAACATTT‑ACCTCTGGCCTTCCAGAAACAGTTGTATTCCgggggctct                                                                                             >  2:349479/1‑243 (MQ=255)                                                                                                                                             cgcAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTg                                                                                         <  1:249209/251‑1 (MQ=255)                                                                                                                                                   aCTCACCACTATCGTTGCTCCTGTAATGCCTTGCCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTcc                                                                                    <  2:129731/250‑1 (MQ=255)                                                                                                                                                          acTATCGTTGCTCCCGTACTGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATTCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCCCTGGATATGGGGCACGGCCCAATTCTTGCCGTTGTCGCGGCGGTCACCACCGGCGTGTTTGGCGCCGTTTTGGGCGGTTTGTTCTGGCAACTTAT‑CCCACGGGTTTTCCAGCATGAGTTTTATGCCGGTGgctctgttgccccccccgtccg                                                                            >  2:160030/1‑233 (MQ=255)                                                                                                                                                                  tGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGGTTTTCGCGATATGTTCTGTAAACGCAT‑CCCACTGGGATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATcc                                                                    >  1:14713/1‑250 (MQ=255)                                                                                                                                                                               ccTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGCCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGGTTGGCGGCGCTTTGCGCGATATGGTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGGGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACtt                                                       >  1:416098/1‑250 (MQ=255)                                                                                                                                                                                   aCCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGtt                                                   <  2:88481/251‑1 (MQ=255)                                                                                                                                                                                                     tGGTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTTCGCGATATGTTCTGTAAACGTAT‑CCCAATGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGGGCTGTACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGt                                  >  1:55762/1‑250 (MQ=255)                                                                                                                                                                                                      ggTGCTCGATGCGCTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCAACGGCGTGTTTGGCGGCGTTTTGCGCGAAATGTTCTGTGAACGTATACCCAC‑GGTATTCCAGAAAGAGTTGTATGCCGGGGTCTCAATTTCCTTCGCCGGGCTGTGCATCCCGCTGCAACCCTATGTTTCTAACCatgatggggaa                                 >  2:883/1‑245 (MQ=255)                                                                                                                                                                                                            cGATGCGCTTGGACTGGTGGGTTTGTCTATCATCGGCTCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTAtc                           <  2:436836/250‑1 (MQ=255)                                                                                                                                                                                                                gcgcTTGGACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCa                       <  2:17052/250‑1 (MQ=255)                                                                                                                                                                                                                    ttggACTGGTGGTCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCCTGGTTGGCGGCGTTTTGCGGGGTATGTTCTGTAAAAGTCT‑CCCCCTGGGATTCCAGGAAGAGTTGTATTCCGGTGTATCCTTTTACTCCGCCCTGCTGTACATCGCGCTGAAACCCCATGGTTCTACACATGATGTGGCAAATTTCTCCCCCCTg                  >  2:55069/3‑251 (MQ=255)                                                                                                                                                                                                                     tggACTGGTGGGCTTTTCTATCATCGGCGCTCAGGTTGCAATGGATATGGGGCACGGCCCAATTAGTGCCGTTGTCGCGGCGGTAACCACCGGCGTGGTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCt                   <  2:393199/248‑1 (MQ=255)                                                                                                                                                                                                                      ggACTGGTGGTCCTTTCTATCACCGGCGTTCAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTTGTTGGCGGCGTGTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGGGCTGTACAGCGCGCTGCAACTCTATGTTGCTAACCATGATGGGGTAATTATCGCCAACCTGGt                <  2:177315/251‑1 (MQ=255)                                                                                                                                                                                                                           ggtggtCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTAtt             <  1:126295/249‑1 (MQ=255)                                                                                                                                                                                                                            gtggtCTTTTCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCgg          <  2:14713/251‑1 (MQ=255)                                                                                                                                                                                                                                    ttCTATCATCGGCGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACCGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTCTGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTg  >  1:74581/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                CGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTCGGACTGGTGGTCTTTTCTATCATCGGGGCACAGGTTGCACTGGATATGGGGCACGGCCCAATTATTGCCGTTGTCGCGGCGGTAACCACCGGCGTGTTTGGCGGCGTTTTGCGCGATATGTTCTGTAAACGTAT‑CCCACTGGTATTCCAGAAAGAGTTGTATGCCGGTGTCTCATTTGCCTCCGCCGTGCTGTACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTG  >  NC_000913/3818946‑3819423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑