Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,818,942 A→G G23G (GGA→GGG)  yicG → PF03458 family inner membrane protein YicG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,818,9420AG96.7% 84.9 / ‑5.9 30G23G (GGA→GGG) yicGPF03458 family inner membrane protein YicG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (17/12);  minor base T (1/0);  total (18/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑CTGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCCCTCCCCTTAAAAAATCTCAATCGTGACAATGCGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTCGGACTGGTGGTCTTT  >  NC_000913/3818704‑3819173
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                       
ttttCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATg                                                                                                                                                                                                                             >  2:232032/1‑251 (MQ=255)
   tCTTATGCATTTTCTCAGAAAGGAAGACCGAAAAATGCATCTACT‑CTACCGAACATAGCGATTTCCCCTAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACACATCGCTACCCTGCCAGACAGATTTTTAGGTAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGTCGGCCGGTCGTCGCCGCATGGAt                                                                                                                                                                                                                          >  2:424412/1‑251 (MQ=255)
     ttATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATAc                                                                                                                                                                                                                        >  2:219269/1‑251 (MQ=255)
         gCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACAttt                                                                                                                                                                                                                    >  1:45289/1‑251 (MQ=255)
                           agTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCg                                                                                                                                                                                                  >  2:126295/1‑251 (MQ=255)
                               agaagaATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCg                                                                                                                                                                                              >  1:88481/1‑251 (MQ=255)
                                   gaaTGCATCT‑ACGCAACAGAAACTAGCGATTTCACATAACTACAAGATATCAATTTCCTCTCACCTTAAAAAATCTCAATCGAGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCAGGGCGACGCCGCATGGATACCTTTGGAGTGATTATTATTGCGACAGCCAc                                                                                                                                                                                          >  2:383755/1‑251 (MQ=255)
                                                   aCAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAg                                                                                                                                                                            <  2:45289/250‑1 (MQ=255)
                                                       aaaaTAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCa                                                                                                                                                                       <  1:379568/251‑1 (MQ=255)
                                                                   tCACATATCTACAAGTTATCAATTTCCTCTCACCTGAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTGGGCATTACTGCCGAAGCCAGGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCaa                                                                                                                                                                                     <  2:140608/225‑1 (MQ=255)
                                                                   tCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCaa                                                                                                                                                                                     >  1:140608/1‑225 (MQ=255)
                                                                         aaCTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTctgct                                                                                                                                                      <  2:120856/250‑1 (MQ=255)
                                                                                    aTCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATcc                                                                                                                                          >  1:243762/1‑251 (MQ=255)
                                                                                        aTTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTc                                                                                                                                      >  1:436836/1‑251 (MQ=255)
                                                                                          ttCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAATTCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGATGTTACACATTTTGTATATGGTTGGCATTACTGCCGAAGCCATGACGGGAGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGACACCGCAATTGTCGGCGGGTCGGTGCTGGTTATTCTTCTGGGCACCTATACGCTCgg                                                                                                                                    >  2:141833/1‑251 (MQ=255)
                                                                                              cctcacctTAAAAAATCGCAACCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTTCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCTCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGAGATTCTGCTGGGCCACTATCCGCTCGGCTgg                                                                                                                                <  2:415977/251‑1 (MQ=255)
                                                                                                cctcctTAAAAAATCTCAACCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTCCTGCCGAAGCCATGACGGGGGCGCTGGCGGCCGGGCGTCGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGt                                                                                                                              <  2:126550/248‑1 (MQ=255)
                                                                                                 cacctTAAAAAATCTCAAGCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTgg                                                                                                                                <  1:219269/246‑1 (MQ=255)
                                                                                                  accttccaaaaTCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCCCCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTc                                                                                                                             >  2:182965/8‑250 (MQ=255)
                                                                                                       aaaaaaTCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAacac                                                                                                                       <  1:232032/251‑1 (MQ=255)
                                                                                                                    cGTGACAATACGCACAAATCGCTACCCTGCCAGACCGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATAtgtgc                                                                                                          >  1:102221/1‑250 (MQ=255)
                                                                                                                       gACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGAtt                                                                                                        <  1:18705/250‑1 (MQ=255)
                                                                                                                               gcACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCt                                                                                               >  2:324878/1‑251 (MQ=255)
                                                                                                                                                  cagacagaTTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGCATATGTGATTATCGTCGCTACCGCCGCAGTACTcaccc                                                                            >  2:88530/1‑250 (MQ=255)
                                                                                                                                                                        cacagggtgatACACATTTGGTATTGGGTTGGCATTCCGGCCGAAGCCATGGGGGGGGCTCTGGCGGCCGGGCGACGCCGCAGGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATAGGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGcc                                                      <  2:102221/241‑1 (MQ=255)
                                                                                                                                                                                 ttACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTgcg                                             >  2:111470/1‑251 (MQ=255)
                                                                                                                                                                                  tacacaaattGTATTTGAATGGCATTACTGCCGATGCCATGACGCGGGCTATGGAGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCAACGCAAATGGCGGAGGGTCAGTGCGCGATATTCTGCTGAGCAACTATCCGCTCGGCTGGGTCCACCACCCGGCATATGTGCTTATCGTCGCTACCGCCGCAGTACTCACCCCTATCGTTGATCCTGTAATGCCTTACCTgcgc                                            >  2:196929/9‑251 (MQ=255)
                                                                                                                                                                                                                  gAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCAc                                                                                                                                               <  2:137523/120‑1 (MQ=255)
                                                                                                                                                                                                                  gAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCAc                                                                                                                                               >  1:137523/1‑120 (MQ=255)
                                                                                                                                                                                                                            ggggggCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTAGCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTTGGACTGGTGGTCttt  <  1:182965/250‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                       
TTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑CTGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCCCTCCCCTTAAAAAATCTCAATCGTGACAATGCGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCACCGCAATTGGCGGAGGGTCAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAACACCCGGAATATGTGATTATCGTCGCTACCGCCGCAGTACTCACCACTATCGTTGCTCCTGTAATGCCTTACCTGCGCAAAGTGTTTCTGGTGCTCGATGCGCTCGGACTGGTGGTCTTT  >  NC_000913/3818704‑3819173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑