Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 246,747 G→T S12S (TCG→TCT)  yafL → NlpC/P60 family protein YafL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913246,7470GT97.7% 145.6 / NA 44S12S (TCG→TCT) yafLNlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (28/15);  total (28/16)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCT  >  NC_000913/246508‑246989
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  
tATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGtt                                                                                                                                                                                                                                         >  2:8010/1‑251 (MQ=255)
        gtgCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGATTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGAtttgt                                                                                                                                                                                                                                  <  2:78582/250‑1 (MQ=255)
          gCTGTATATTTATTCAGTTTGAAATGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGAGGTTTTTACATAATTAACGGAAATATATATGTTAATTTTATAATAAGGGTTTATTAAGTTTGTTCAGAAACTATCCGGGATACACTGCAACATTAAAGCATGACCAGCTATTATGGAGTATGTCCTTGCCTTCGATTCCATCCTTTGTATTGTCTTTATTACTGTTTAgttgtttg                                                                                                                                                                                                                               <  2:25803/251‑1 (MQ=255)
          gCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCTTCGATTCCATCCTTTGTATTTTCTGGATTACTGTTGAtttgttt                                                                                                                                                                                                                                <  2:274034/250‑1 (MQ=255)
               atatTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATTATTAATTTAATAAACGGATGTTTTGACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATGCACTGCATCATTAAAGCATGACCTGCCATTAGTGAGTAGGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGtt                                                                                                                                                                                                                          <  2:115318/251‑1 (MQ=255)
                         aGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTAGTGTCTGGATTACTGTTGATTTGTGTGCCGTTTTCTGCAttt                                                                                                                                                                                                                <  1:336803/251‑1 (MQ=255)
                                  tGTAGCTCAAATGAGCAACTGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTCAATAATTAACTGCAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCACCATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGcc                                                                                                                                                                                                       >  2:275419/1‑251 (MQ=255)
                                                  aagagggccaatcactGAGTAATTTAATAAGCGGATGTTTTAACACCATTCACTGAAATATGTATGTTAAAGTTATACTAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGTTACACTGCAACATTAAAGAATGCCCAGCCATTATTGAGTATGTCCTTACCGTCGATTCCATCCTATGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCCCCACATCCCATAtct                                                                                                                                                                                       >  2:388373/16‑251 (MQ=255)
                                                            aTCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCt                                                                                                                                                                              >  2:332470/1‑250 (MQ=255)
                                                                aTGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGCGTATGTCCTTGCCGTCGCTTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTAcgcc                                                                                                                                                                         >  2:428334/1‑251 (MQ=255)
                                                                         ttAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCcgcccgcc                                                                                                                                                                  >  1:56731/1‑249 (MQ=255)
                                                                                            aCATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGCTTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTgcgc                                                                                                                                             >  2:101655/1‑251 (MQ=255)
                                                                                                    aaCTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAAAAACGATCCGGGATACACTGCAACATTAAAGCATGCCCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTttatt                                                                                                                                      <  1:139179/250‑1 (MQ=255)
                                                                                                        aaaaTATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAAc                                                                                                                                 <  1:101655/250‑1 (MQ=255)
                                                                                                          aaTATATATGTTAATTTTCTACTAATGGTTTATTACGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCAATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAAc                                                                                                                                 >  2:291792/1‑249 (MQ=255)
                                                                                                                 atGTTAATTTTATAATAATGGTTTATTACGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACAAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTTCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCACCAGCGGATGCAAAACCGTGCGCGTTTCTTCAACCAGTACCaa                                                                                                                         >  2:265544/1‑250 (MQ=255)
                                                                                                                   gTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGccacca                                                                                                                                                                                                   >  2:104606/1‑174 (MQ=255)
                                                                                                                   gTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTGTTCTGCATTTGCCAGCGccacca                                                                                                                                                                                                   <  1:104606/174‑1 (MQ=255)
                                                                                                                       aTTTTATAATAAAGGTTAATTAAGTTTGTTCCGAAAAGATCCGGGATACACTGCACCATTACAGAATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATc                                                                                                                  >  2:228274/1‑251 (MQ=255)
                                                                                                                         tttATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAACCTCATCTg                                                                                                                >  2:226270/1‑251 (MQ=255)
                                                                                                                              aataatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACCCTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGAATGCAACACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaaa                                                                                                            >  2:246581/1‑250 (MQ=255)
                                                                                                                                 aatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTTCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCTTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCTCCACCACAGCACATATCTCTTTCATCGACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCa                                                                                                        <  1:194418/251‑1 (MQ=255)
                                                                                                                                   tGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAgg                                                                                                      >  1:109823/1‑251 (MQ=255)
                                                                                                                                   tGGTTTACTAAGTATGATCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTCTGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGCTGCAAAACCGTGCGCGTTTATTAACACAGTACCAAAATCATCTGAAAAAGCAgg                                                                                                      >  2:21618/1‑251 (MQ=255)
                                                                                                                                    gttttATTAAGTTTGTTCAGAAACTATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTTTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGTATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGc                                                                                                     <  1:91963/249‑1 (MQ=255)
                                                                                                                                         attaAGTTTGTTCAGCAACGATCCGGGATACACTGCACCATTAAAGCATGACCCGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCACGCCAGCGGATGCAAAACCGTGAGCGTTTCTTAAAACAGTACCAAACTCATCTGAACAAGCAGGCCAGCt                                                                                                >  2:351104/1‑251 (MQ=255)
                                                                                                                                         attaAGGTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTGGCCGTCTATTCCATCCTTTGTAGTGTCTGGATTACTTTTGATTTGTTGGCCTTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCt                                                                                                <  1:260487/251‑1 (MQ=255)
                                                                                                                                           taAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCtat                                                                                              >  1:132263/1‑251 (MQ=255)
                                                                                                                                            aaGTTTGTTTAGAAACGATCCGGGATACACGGCAACATAAAAGCATGCCCAGCCATTATGGAGTATGTCGTTGCCGTCGATTCCATCCTTTGTATGGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTCCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTTCCAAACTCATCTGAAAAAGCAGTCCAGCtat                                                                                              <  1:94127/250‑1 (MQ=255)
                                                                                                                                                     aaGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACAGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGATATATTGAGGa                                                                                      >  2:116170/2‑249 (MQ=255)
                                                                                                                                                         aaCGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCa                                                                                 >  1:101796/1‑250 (MQ=255)
                                                                                                                                                          ccGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATCCCATCCTTTGTATTGTCTGGATGACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTGCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAAt                                                                               <  1:246581/250‑1 (MQ=255)
                                                                                                                                                                     tACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCa                                                                     >  1:238989/1‑250 (MQ=255)
                                                                                                                                                                                 ttAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACg                                                        >  1:313171/1‑251 (MQ=255)
                                                                                                                                                                                       caggcccAGCCATTATTGAGTATGTCCTTGCTGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTGGCCAGCGCCACCACATCTCATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTGATTAAAACAGTACCAAACTCATCTGAAAAAGCa                                                                                                        <  1:335615/192‑1 (MQ=255)
                                                                                                                                                                                       cATGACCAGCCATTATTGAGTATGTCCTTGCTGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCCTCTGAAAAAGCa                                                                                                        >  2:335615/1‑197 (MQ=255)
                                                                                                                                                                                        aTGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCCTCTGAAAAAGCAGGCCAGCTATATTGTGGACGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGcac                                                 >  1:31136/1‑251 (MQ=255)
                                                                                                                                                                                        aTGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCAACGCCAGcac                                                 >  1:164732/1‑251 (MQ=255)
                                                                                                                                                                                           aCCAGCCCTTATTGAGAATGTCATTGCCGTCGATTCCATCCTTTGTATTGTCTGGGTTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTCCCAAACTCATCTGAAAAAGCAGGCCAGCTATCTTGTGGAAGGCCATGCCGAACGCAAAAGGGCGCTACGCCAGCACAAc                                              >  2:46470/1‑251 (MQ=255)
                                                                                                                                                                                             cAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGACAGCAAAAGGGCGCTACGCCAGCACAACCg                                            >  2:364304/1‑251 (MQ=255)
                                                                                                                                                                                                     attGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGa                                    <  1:1415/251‑1 (MQ=255)
                                                                                                                                                                                                             tGTCCCTGCCGTCGCTTAAATCATTTGTATTGTCTGGATTACTGTTGCTTTGTTTGCAGTTTTCTTCATTTGCCCGCGCCACCACATAACATATCTCTTTCAGCTACGCCGACCGCCACCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGCAAGCAAAAGGGCGCTAAGCCAGCACAACCGGGAGCAGATAAAAc                              >  2:284125/1‑249 (MQ=255)
                                                                                                                                                                                                                     ccGTCGATTCCATCCTTTGTAATGTATGGATTACTGTTGCTTTGTTTGCAGTTTTCTTCATTTGCAAGCGCCACCACCTCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATcc                       >  2:190225/1‑248 (MQ=255)
                                                                                                                                                                                                                                       gTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCGTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCt  <  1:265544/251‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  
TATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCT  >  NC_000913/246508‑246989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑