Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,818,331	A→G	N191S (AAT→AGT)	dinD →	DNA damage‑inducible protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,818,331	0	A	G	100.0%	100.4 / NA	30	N191S (AAT→AGT)	dinD	DNA damage‑inducible protein D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/17);  total (13/17)

CTATCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACATAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAGCTATTCATCAGCGGAAGGGGCTGAAAAAGAATCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCTAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCAAACACCACACACTTTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGA  >  NC_000913/3818088‑3818574                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    cTATCCAGACCCGACGGCAGGAGCTGGCTGACGATGAAGCTTTCAGGCAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCaga                                                                                                                                                                                                                                                >  2:137511/1‑249 (MQ=255)          cccGACGGCAGGAGCTGGCGGACGATGAAGCTTTCAGGCAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAATGGGCTGAAAAAGAGTCAGAAGAGCCTGGAt                                                                                                                                                                                                                                     <  2:161031/251‑1 (MQ=255)                       gctggCTGACGATGAAGCTTTCAGGCAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCaa                                                                                                                                                                                                                        <  2:12647/247‑1 (MQ=255)                              gACGATGAAGCTTTCAGGCAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGACTTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGCCAGCTATCGATTTCGCCATATTTCAGAATCATGGTTAACGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACt                                                                                                                                                                                                                 >  1:72087/1‑251 (MQ=255)                                             aggCAACTTCGTGAAGACGAAAATCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTGTCGATTTTGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGGTGAGATCAGAAAGCCATCCATCAGCGGAGGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCtt                                                                                                                                                                                                  <  1:290851/249‑1 (MQ=255)                                                cAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCg                                                                                                                                                                                               >  2:360097/1‑251 (MQ=255)                                                         gAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAAGTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTcccc                                                                                                                                                                                       <  2:97317/250‑1 (MQ=255)                                                                aaaaGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCAATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGCACTGGCGGCGAATCTCTTTCGAGCTACCCAAACaga                                                                                                                                                                                >  1:16846/1‑250 (MQ=255)                                                                   aGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAgaagaa                                                                                                                                                                            <  1:193388/251‑1 (MQ=255)                                                                      gTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGaaaa                                                                                                                                                                          >  1:315286/1‑250 (MQ=255)                                                                                            gAAAGAACATAACATACACGTGGGTGGGGCAGCGCAGCAAGCGGGGGGAGCTACAGCGAGCGATTTCGCCATAGTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTTGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTGCAACAGAACTGGCGGCTAATCGCTTTCGAGCGACCCAAACAGAAGAAAAACTCAAGCGGGATGGCgggtat                                                                                                                                                    <  2:72087/250‑6 (MQ=255)                                                                                                  aCATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAAGTCAAAAc                                                                                                                                             <  2:54891/251‑1 (MQ=255)                                                                                                                       gtcagcgcagcaAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTAGCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAACACCACACACt                                                                                                                        <  2:241655/249‑1 (MQ=255)                                                                                                                            cgcagcaAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTGCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGAc                                                                                                                   <  1:217436/249‑1 (MQ=255)                                                                                                                                                        cgATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTc                                                                                       >  2:74712/2‑251 (MQ=255)                                                                                                                                                              cGCCATATTTCAGAATCTTGGTTACCGGGGGCTGTATGGCGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGCAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTCATACCACCCACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTCAGGAAc                                                                                 >  2:392893/1‑251 (MQ=255)                                                                                                                                                                                         ggggCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGc                                                      <  1:360097/251‑1 (MQ=255)                                                                                                                                                                                              tGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGCAAAAGAGTCAGAAGCTCCTGGATCATATGGGTTCAACCGAACTGGCGGCGAATCTCTTTCGAGCTACCCCAACAGAAGAAAAACTCAAGCGGGATGGCGTGCATTCAAACCagcaa                                                                                                                                        >  2:24638/1‑164 (MQ=255)                                                                                                                                                                                              tGTATGGTGGATTAGATCAGAAAGCCAACCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTGCCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACagcaa                                                                                                                                        <  1:24638/164‑1 (MQ=255)                                                                                                                                                                                                           aGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAACAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGCTGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGAc                                                                                                                   >  2:296326/1‑172 (MQ=255)                                                                                                                                                                                                           aGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGAc                                                                                                                   <  1:296326/172‑1 (MQ=255)                                                                                                                                                                                                             aTCAGAAAGCGATCCATCAGCGGAAGGTGCTGAAAAAGAGTCATAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTGTCGAGCTACCCAAACATAACAAAAACTCAAGCGGGATGGCGTGAATGCAAAACAGCAAGCTAATACCACACACGGTGACGTGTGTAGCAAGGTGAGGCAAACCAATCAGGAACTTGGCGGAATGATGCCTTAGGAATTGCCGACCCCGGAGGgcagcagc                                  <  2:249945/251‑3 (MQ=255)                                                                                                                                                                                                                     gccatccatcaGCGGAAGGGGCTGAAACAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATg                                                                   >  2:21979/7‑210 (MQ=255)                                                                                                                                                                                                                     gccatccatcaGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATg                                                                   <  1:21979/204‑1 (MQ=255)                                                                                                                                                                                                                        atccatcaGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCATGAGGAGTTGCCGACCCCGCAGGTCAGCGTCAAGCAGTTgg                        >  1:22960/4‑250 (MQ=255)                                                                                                                                                                                                                                  ggAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAAAAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAgtgt              >  1:126550/1‑250 (MQ=255)                                                                                                                                                                                                                                   gAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTaa            <  1:432086/251‑1 (MQ=255)                                                                                                                                                                                                                                          cTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGCATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGc                                                                                                >  2:304305/1‑160 (MQ=255)                                                                                                                                                                                                                                          cTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGc                                                                                                <  1:304305/160‑1 (MQ=255)                                                                                                                                                                                                                                             aaaaaGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCGAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACaga  <  1:108899/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    CTATCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACATAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAGCTATTCATCAGCGGAAGGGGCTGAAAAAGAATCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCTAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCAAACACCACACACTTTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGA  >  NC_000913/3818088‑3818574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑