Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,818,305	T→C	I182I (ATT→ATC)	dinD →	DNA damage‑inducible protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,818,305	0	T	C	100.0%	79.2 / NA	26	I182I (ATT→ATC)	dinD	DNA damage‑inducible protein D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/15);  total (11/15)

CATTGCGGCAGGGCAGACTTATTTTGCTATCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACATAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAGCTATTCATCAGCGGAAGGGGCTGAAAAAGAATCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCTAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCAAACACCACACACTTTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATC  >  NC_000913/3818062‑3818542                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              taTTGCGGCATGGCAAACTTATTTTGCTATCCAGACCCGACGGCAGGAGCTGGCTTACGATGAAGCTTTCAGGCAATTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAACTGAATTGAAAGAACATAACAAACAATTGGTTGAGGCATCGCAGCAAGCTGGTGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAg                                                                                                                                                                                                                                         <  1:231213/249‑1 (MQ=255) taTTGCGGCAGGGCAAACTTATTTTGCTATCCAGACCCGACGGCAGGAGCTGACTGACGATGAAGCTTTCAGGCAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGATGAAGGAGCGCCAGCTATCGATTACGACATATTTCAGAATCATGGTTACAGGGGGCTGTATGGAGGATTAGATCAGAAAGCGATCCATCAGc                                                                                                                                                                                                                                        >  1:287923/2‑251 (MQ=255)            ggcaaACTTATTTTGCTATCCAGACCCGACGGCAGGAGCTGGCTGACGATGAAGCTTTCAGGCAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTg                                                                                                                                                                                                                             >  1:108379/1‑251 (MQ=255)                       tttGCTAGCCAGACCCGACGGCAGGAGCTGGCTGACGATGAAGCTTTCAGGCAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTGCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGagtc                                                                                                                                                                                                                   <  2:109449/250‑4 (MQ=255)                           cTATCCAGACCCGACGGCAGGAGCTGGCTGACGATGAAGCTTTCAGGCAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCaga                                                                                                                                                                                                                >  2:137511/1‑249 (MQ=255)                                    cccGACGGCAGGAGCTGGCGGACGATGAAGCTTTCAGGCAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAATGGGCTGAAAAAGAGTCAGAAGAGCCTGGAt                                                                                                                                                                                                     <  2:161031/251‑1 (MQ=255)                                                 gctggCTGACGATGAAGCTTTCAGGCAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCaa                                                                                                                                                                                        <  2:12647/247‑1 (MQ=255)                                                        gACGATGAAGCTTTCAGGCAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGACTTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGCCAGCTATCGATTTCGCCATATTTCAGAATCATGGTTAACGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACt                                                                                                                                                                                 >  1:72087/1‑251 (MQ=255)                                                                       aggCAACTTCGTGAAGACGAAAATCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTGTCGATTTTGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGGTGAGATCAGAAAGCCATCCATCAGCGGAGGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCtt                                                                                                                                                                  <  1:290851/249‑1 (MQ=255)                                                                          cAACTTCGTGAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCg                                                                                                                                                               >  2:360097/1‑251 (MQ=255)                                                                                   gAAGACGAAAAGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAAGTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTcccc                                                                                                                                                       <  2:97317/250‑1 (MQ=255)                                                                                          aaaaGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCAATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGCACTGGCGGCGAATCTCTTTCGAGCTACCCAAACaga                                                                                                                                                >  1:16846/1‑250 (MQ=255)                                                                                             aGCGTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAgaagaa                                                                                                                                            <  1:193388/251‑1 (MQ=255)                                                                                                gTCTTTTTCTGAGAAATGAATTGAAAGAACATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGaaaa                                                                                                                                          >  1:315286/1‑250 (MQ=255)                                                                                                                      gAAAGAACATAACATACACGTGGGTGGGGCAGCGCAGCAAGCGGGGGGAGCTACAGCGAGCGATTTCGCCATAGTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTTGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTGCAACAGAACTGGCGGCTAATCGCTTTCGAGCGACCCAAACAGAAGAAAAACTCAAGCGGGATGGCgggtat                                                                                                                    <  2:72087/250‑6 (MQ=255)                                                                                                                            aCATAACAAACAATTGGTTGAGGCAGCGCAGCAAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAAGTCAAAAc                                                                                                             <  2:54891/251‑1 (MQ=255)                                                                                                                                                 gtcagcgcagcaAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTTCAGAATCATGGTTAGCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAACACCACACACt                                                                                        <  2:241655/249‑1 (MQ=255)                                                                                                                                                      cgcagcaAGCTGGGGGAGCGACAGCTATCGATTTCGCCATATTGCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGAc                                                                                   <  1:217436/249‑1 (MQ=255)                                                                                                                                                                                  cgATTTCGCCATATTTCAGAATCATGGTTACCGGGGGCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTc                                                       >  2:74712/2‑251 (MQ=255)                                                                                                                                                                                        cGCCATATTTCAGAATCTTGGTTACCGGGGGCTGTATGGCGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGCAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTCATACCACCCACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTCAGGAAc                                                 >  2:392893/1‑251 (MQ=255)                                                                                                                                                                                                                   ggggCTGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGACGTGGGTAGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGc                      <  1:360097/251‑1 (MQ=255)                                                                                                                                                                                                                        tGTATGGTGGATTAGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGCAAAAGAGTCAGAAGCTCCTGGATCATATGGGTTCAACCGAACTGGCGGCGAATCTCTTTCGAGCTACCCCAACAGAAGAAAAACTCAAGCGGGATGGCGTGCATTCAAACCagcaa                                                                                                        >  2:24638/1‑164 (MQ=255)                                                                                                                                                                                                                        tGTATGGTGGATTAGATCAGAAAGCCAACCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTGCCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACagcaa                                                                                                        <  1:24638/164‑1 (MQ=255)                                                                                                                                                                                                                                     aGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAACAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGCTGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGAc                                                                                   >  2:296326/1‑172 (MQ=255)                                                                                                                                                                                                                                     aGATCAGAAAGCCATCCATCAGCGGAAGGGGCTGAAAAAGAGTCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCTAATACCACACACTTTGAc                                                                                   <  1:296326/172‑1 (MQ=255)                                                                                                                                                                                                                                       aTCAGAAAGCGATCCATCAGCGGAAGGTGCTGAAAAAGAGTCATAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCGAATCTCTGTCGAGCTACCCAAACATAACAAAAACTCAAGCGGGATGGCGTGAATGCAAAACAGCAAGCTAATACCACACACGGTGACGTGTGTAGCAAGGTGAGGCAAACCAATCAGGAACTTGGCGGAATGATGCCTTAGGAATTGCCGACCCCGGAGGgcagcagc  <  2:249945/251‑3 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              CATTGCGGCAGGGCAGACTTATTTTGCTATCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACATAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAGCTATTCATCAGCGGAAGGGGCTGAAAAAGAATCAGAAGATCCTGGATCATATGGGTTCAACAGAACTGGCGGCTAATCTCTTTCGAGCTACCCAAACAGAAGAAAAACTCAAGCGGGATGGCGTGAATTCAAAACAGCAAGCAAACACCACACACTTTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATC  >  NC_000913/3818062‑3818542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑