Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,817,739	T→C	intergenic (+200/‑21)	yicC → / → dinD	putative RNase adaptor protein YicC/DNA damage‑inducible protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,817,739	0	T	C	100.0%	35.7 / NA	12	intergenic (+200/‑21)	yicC/dinD	putative RNase adaptor protein YicC/DNA damage‑inducible protein D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/7);  total (5/7)

ATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCC‑ACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATTGATATCACATGGAGTGGGCAATGAACGAACATCATCAACCTTTTGAAGAGATAAAACTGATTAATGCAAACGGAGC  >  NC_000913/3817501‑3817815                                                                                                                                                                                                                                                |                                                                             attgaGCAGATGCGCGAGCAGATTCAGAATATTGAATAACGCGTAACACTAAGTCTTACTTGTCAGCCTTCACAAGTGCCGCCCACTCAAACAGAGCGGCATTTTGCTTCCCCGCAAC‑TTCAATGCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTTAGATACTCACAACTGTATATAAATACAGGTACAGAATTACTTCCTGTGCAATCAATATCCCATgg                                                                  <  2:389470/251‑1 (MQ=255)      gCAGATGCTCGAGCAGATTCAGAATATTGAGGAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGCCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTGTTTTTGCCCAGCCCACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATGTACTTTCTTTGCAATCGATATCACATGGAGTgg                                                             <  1:141969/251‑1 (MQ=255)                            aatattgagtAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGCCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATGAACGAACATCATCAAc                                       >  2:193880/10‑251 (MQ=255)                                             aacactaaTTCTTACTTTTCAGCCTTCACAAATGCCGCCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATGAACGAACATCATCAACCTTTTGAAGAAATacgg                      <  1:107278/245‑4 (MQ=255)                                                      tCTTACTTTTCAGCCTTCACAAATGCCGCCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATGAACGAACATCATCAACCTTTTGAAGAAATacggcattatgg              <  1:193880/250‑12 (MQ=255)                                                               tcagCCTTCACAAATGCCGCCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATGAACGAACATCATCAACCTTCTGAAGAAATacggcattatggcactgaagg     <  1:165763/247‑21 (MQ=255)                                                                  gCCTTCACAAATGCCGCCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATGAACGAACATCATCAACCTTTTGAAGAAATacggcattatggcactgaagggca  >  2:328706/1‑227 (MQ=255)                                                                  gCCTTCACAAATGCCGCCCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATGAACGAACATCATCAACCTTTTGAAGAAATacggcattatggcactgaagggca  >  2:354197/1‑227 (MQ=255)                                                                                                            ttcCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATgaac                                                    >  1:135247/1‑158 (MQ=255)                                                                                                            ttcCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATgaac                                                    <  2:135247/158‑1 (MQ=255)                                                                                                                                                                          cAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTAGCTTTGCAATCGATATCACATGGAGTGGGCAATGAACGAACATCATCAGCTTTTTGaa                               <  2:401156/118‑1 (MQ=255)                                                                                                                                                                          cAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATAAAGTTACAGATTTACTTTCTTTGCAATCGATATCCAATGGAGTGGGCAATGAACGAACATCATCAACCTTTTGac                               >  1:401156/1‑117 (MQ=255)                                                                                                                                                                                                                                                |                                                                             ATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGCC‑ACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATTGATATCACATGGAGTGGGCAATGAACGAACATCATCAACCTTTTGAAGAGATAAAACTGATTAATGCAAACGGAGC  >  NC_000913/3817501‑3817815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑