Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,817,565	C→A	intergenic (+26/‑195)	yicC → / → dinD	putative RNase adaptor protein YicC/DNA damage‑inducible protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,817,565	0	C	A	100.0%	114.4 / NA	34	intergenic (+26/‑195)	yicC/dinD	putative RNase adaptor protein YicC/DNA damage‑inducible protein D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/19);  total (15/19)

AAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGC‑C‑ACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATTGATATCACATGGAGTGGGCAATGAACGAACATCATCAACCTTTTGAAGAGATAAAACTGATTAA  >  NC_000913/3817322‑3817803                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 aagaaCTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTca                                                                                                                                                                                                                                           <  1:54922/251‑1 (MQ=255)     aCTGGAACGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCGTCACaaa                                                                                                                                                                                                                                       <  2:82895/251‑1 (MQ=255)        ggaccGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATg                                                                                                                                                                                                                                     <  2:309861/246‑1 (MQ=255)          accGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccg                                                                                                                                                                                                                                  <  1:430751/249‑1 (MQ=255)            cGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccgc‑c                                                                                                                                                                                                                               >  1:247803/1‑251 (MQ=255)                   aaGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCa                                                                                                                                                                                                                         >  1:89850/1‑250 (MQ=255)                              aaaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGc                                                                                                                                                                                                              >  1:263765/1‑250 (MQ=255)                              aaaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCa                                                                                                                                                                                                             <  1:418963/251‑1 (MQ=255)                              aaaGAGACGTACAACATTATGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGCCTTTATGATGCAGGAGTTCAAACGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAACGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGCAC‑ACTCAACCAGAGCgtc                                                                                                                                                                                                              >  2:38857/1‑248 (MQ=255)                               aaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccgc‑ccact                                                                                                                                                                                                                           >  2:51998/1‑232 (MQ=255)                               aaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccgc‑ccact                                                                                                                                                                                                                           <  1:51998/236‑5 (MQ=255)                                   gacgtACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCAttttt                                                                                                                                                                                                        <  2:247803/247‑1 (MQ=255)                                    acgtACAACATTATGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTttc                                                                                                                                                                                                       >  1:108936/4‑251 (MQ=255)                                      gtACAACCTTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAACCAGAGCGGCATTTttctt                                                                                                                                                                                                     >  2:378119/2‑251 (MQ=255)                                         caacaTTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTccc                                                                                                                                                                                                  >  2:17657/1‑251 (MQ=255)                                                      aaaaaaGCAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAAtt                                                                                                                                                                                    >  2:213254/1‑251 (MQ=255)                                                             aagCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGtttt                                                                                                                                                                             >  2:288378/1‑251 (MQ=255)                                                                                      tATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCaa                                                                                                                                                                                      >  1:427766/1‑217 (MQ=255)                                                                                             cAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTGGCGATGtttttt                                                                                                                                             <  1:368457/251‑1 (MQ=255)                                                                                                       aCCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGcac                                                                                                                                   <  2:89850/251‑1 (MQ=255)                                                                                                                          tGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACt                                                                                                                >  1:374473/1‑251 (MQ=255)                                                                                                                             gTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGGAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTGCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTcac                                                                                                             <  1:159501/251‑1 (MQ=255)                                                                                                                                  aGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTg                                                                                                        <  2:263765/251‑1 (MQ=255)                                                                                                                                       aTCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCTGCATTTTGCTTCCCCGCAAct                                                                                                                                                                                           <  2:404734/164‑2 (MQ=255)                                                                                                                                       aTCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAct                                                                                                                                                                                           >  1:404734/1‑163 (MQ=255)                                                                                                                                          aaTGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATTCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATGTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATaaa                                                                                                <  1:56867/251‑1 (MQ=255)                                                                                                                                                  aGTGACAACCTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCg                                                                                                                                                                                                                >  2:420683/1‑132 (MQ=255)                                                                                                                                                  aGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCg                                                                                                                                                                                                                <  1:420683/132‑1 (MQ=255)                                                                                                                                                                cgattgAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTActttct                                                                          <  1:58230/246‑1 (MQ=255)                                                                                                                                                                                    attgaGCAGATGCGCGAGCAGATTCAGAATATTGAATAACGCGTAACACTAAGTCTTACTTGTCAGCCTTCACAAGTGCCGC‑CCACTCAAACAGAGCGGCATTTTGCTTCCCCGCAAC‑TTCAATGCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTTAGATACTCACAACTGTATATAAATACAGGTACAGAATTACTTCCTGTGCAATCAATATCCCATgg                                                      <  2:389470/251‑1 (MQ=255)                                                                                                                                                                                         gCAGATGCTCGAGCAGATTCAGAATATTGAGGAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTGTTTTTGCCCAGCCCACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATGTACTTTCTTTGCAATCGATATCACATGGAGTgg                                                 <  1:141969/251‑1 (MQ=255)                                                                                                                                                                                                               aatattgagtAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATGAACGAACATCATCAAc                           >  2:193880/10‑251 (MQ=255)                                                                                                                                                                                                                                aacactaaTTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATGAACGAACATCATCAACCTTTTGAAGAAATacgg          <  1:107278/245‑4 (MQ=255)                                                                                                                                                                                                                                         tCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATGAACGAACATCATCAACCTTTTGAAGAAATacggcattatgg  <  1:193880/250‑12 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 AAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGC‑C‑ACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATTGATATCACATGGAGTGGGCAATGAACGAACATCATCAACCTTTTGAAGAGATAAAACTGATTAA  >  NC_000913/3817322‑3817803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑