Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,817,548	G→A	intergenic (+9/‑212)	yicC → / → dinD	putative RNase adaptor protein YicC/DNA damage‑inducible protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,817,548	0	G	A	100.0%	117.1 / NA	35	intergenic (+9/‑212)	yicC/dinD	putative RNase adaptor protein YicC/DNA damage‑inducible protein D
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/19);  total (16/19)

TGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGC‑C‑ACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATTGATATCACATGGAGTGGGCAATGAACGAACATCATCAAC  >  NC_000913/3817317‑3817778                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         tGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTcag                                                                                                                                                                                                                        <  2:292365/250‑3 (MQ=255)      aagaaCTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTca                                                                                                                                                                                                                  <  1:54922/251‑1 (MQ=255)          aCTGGAACGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCGTCACaaa                                                                                                                                                                                                              <  2:82895/251‑1 (MQ=255)             ggaccGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATg                                                                                                                                                                                                            <  2:309861/246‑1 (MQ=255)               accGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccg                                                                                                                                                                                                         <  1:430751/249‑1 (MQ=255)                 cGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccgc‑c                                                                                                                                                                                                      >  1:247803/1‑251 (MQ=255)                        aaGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCa                                                                                                                                                                                                >  1:89850/1‑250 (MQ=255)                                   aaaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGc                                                                                                                                                                                     >  1:263765/1‑250 (MQ=255)                                   aaaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCa                                                                                                                                                                                    <  1:418963/251‑1 (MQ=255)                                   aaaGAGACGTACAACATTATGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGCCTTTATGATGCAGGAGTTCAAACGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAACGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGCAC‑ACTCAACCAGAGCgtc                                                                                                                                                                                     >  2:38857/1‑248 (MQ=255)                                    aaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccgc‑ccact                                                                                                                                                                                                  >  2:51998/1‑232 (MQ=255)                                    aaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccgc‑ccact                                                                                                                                                                                                  <  1:51998/236‑5 (MQ=255)                                        gacgtACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCAttttt                                                                                                                                                                               <  2:247803/247‑1 (MQ=255)                                         acgtACAACATTATGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTttc                                                                                                                                                                              >  1:108936/4‑251 (MQ=255)                                           gtACAACCTTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAACCAGAGCGGCATTTttctt                                                                                                                                                                            >  2:378119/2‑251 (MQ=255)                                              caacaTTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTccc                                                                                                                                                                         >  2:17657/1‑251 (MQ=255)                                                           aaaaaaGCAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAAtt                                                                                                                                                           >  2:213254/1‑251 (MQ=255)                                                                  aagCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGtttt                                                                                                                                                    >  2:288378/1‑251 (MQ=255)                                                                             cgtcgtcTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTc                                                                                                                                                                                                                                   <  2:354175/163‑1 (MQ=255)                                                                             cgtcgtcTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTc                                                                                                                                                                                                                                   >  1:354175/1‑163 (MQ=255)                                                                                           tATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCaa                                                                                                                                                             >  1:427766/1‑217 (MQ=255)                                                                                                  cAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTGGCGATGtttttt                                                                                                                    <  1:368457/251‑1 (MQ=255)                                                                                                            aCCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGcac                                                                                                          <  2:89850/251‑1 (MQ=255)                                                                                                                               tGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACt                                                                                       >  1:374473/1‑251 (MQ=255)                                                                                                                                  gTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGGAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTGCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTcac                                                                                    <  1:159501/251‑1 (MQ=255)                                                                                                                                       aGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTg                                                                               <  2:263765/251‑1 (MQ=255)                                                                                                                                            aTCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCTGCATTTTGCTTCCCCGCAAct                                                                                                                                                                  <  2:404734/164‑2 (MQ=255)                                                                                                                                            aTCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAct                                                                                                                                                                  >  1:404734/1‑163 (MQ=255)                                                                                                                                               aaTGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATTCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATGTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATaaa                                                                       <  1:56867/251‑1 (MQ=255)                                                                                                                                                       aGTGACAACCTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCg                                                                                                                                                                                       >  2:420683/1‑132 (MQ=255)                                                                                                                                                       aGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCg                                                                                                                                                                                       <  1:420683/132‑1 (MQ=255)                                                                                                                                                                     cgattgAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTActttct                                                 <  1:58230/246‑1 (MQ=255)                                                                                                                                                                                         attgaGCAGATGCGCGAGCAGATTCAGAATATTGAATAACGCGTAACACTAAGTCTTACTTGTCAGCCTTCACAAGTGCCGC‑CCACTCAAACAGAGCGGCATTTTGCTTCCCCGCAAC‑TTCAATGCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTTAGATACTCACAACTGTATATAAATACAGGTACAGAATTACTTCCTGTGCAATCAATATCCCATgg                             <  2:389470/251‑1 (MQ=255)                                                                                                                                                                                              gCAGATGCTCGAGCAGATTCAGAATATTGAGGAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTGTTTTTGCCCAGCCCACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATGTACTTTCTTTGCAATCGATATCACATGGAGTgg                        <  1:141969/251‑1 (MQ=255)                                                                                                                                                                                                                    aatattgagtAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATCGATATCACATGGAGTGGGCAATGAACGAACATCATCAAc  >  2:193880/10‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         TGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGC‑C‑ACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATTGATATCACATGGAGTGGGCAATGAACGAACATCATCAAC  >  NC_000913/3817317‑3817778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑