Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,817,536	A→G	E287E (GAA→GAG)	yicC →	putative RNase adaptor protein YicC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,817,536	0	A	G	97.2%	117.7 / ‑5.8	36	E287E (GAA→GAG)	yicC	putative RNase adaptor protein YicC
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (15/20);  total (15/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGC‑C‑ACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATTGATATCACATGGAGTGG  >  NC_000913/3817296‑3817756                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                               gctgTCACAACGATTGGACGTTGCCGAATAACTGGACCGCCGCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAGCCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTaa                                                                                                                                                                                                                      <  1:186061/251‑1 (MQ=255)       acaACGAATTGACGTTGCCGAAGAACGGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATAGTGAGTAACTCGTaatactaa                                                                                                                                                                                                                <  1:317455/251‑7 (MQ=255)                      tGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTcag                                                                                                                                                                                                  <  2:292365/250‑3 (MQ=255)                           aagaaCTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTca                                                                                                                                                                                            <  1:54922/251‑1 (MQ=255)                               aCTGGAACGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCGTCACaaa                                                                                                                                                                                        <  2:82895/251‑1 (MQ=255)                                  ggaccGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATg                                                                                                                                                                                      <  2:309861/246‑1 (MQ=255)                                    accGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccg                                                                                                                                                                                   <  1:430751/249‑1 (MQ=255)                                      cGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccgc‑c                                                                                                                                                                                >  1:247803/1‑251 (MQ=255)                                             aaGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCa                                                                                                                                                                          >  1:89850/1‑250 (MQ=255)                                                        aaaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGc                                                                                                                                                               >  1:263765/1‑250 (MQ=255)                                                        aaaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCa                                                                                                                                                              <  1:418963/251‑1 (MQ=255)                                                        aaaGAGACGTACAACATTATGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGCCTTTATGATGCAGGAGTTCAAACGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAACGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGCAC‑ACTCAACCAGAGCgtc                                                                                                                                                               >  2:38857/1‑248 (MQ=255)                                                         aaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccgc‑ccact                                                                                                                                                                            >  2:51998/1‑232 (MQ=255)                                                         aaGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATgccgc‑ccact                                                                                                                                                                            <  1:51998/236‑5 (MQ=255)                                                             gacgtACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCAttttt                                                                                                                                                         <  2:247803/247‑1 (MQ=255)                                                              acgtACAACATTATGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTttc                                                                                                                                                        >  1:108936/4‑251 (MQ=255)                                                                gtACAACCTTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAACCAGAGCGGCATTTttctt                                                                                                                                                      >  2:378119/2‑251 (MQ=255)                                                                   caacaTTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTccc                                                                                                                                                   >  2:17657/1‑251 (MQ=255)                                                                                aaaaaaGCAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAAtt                                                                                                                                     >  2:213254/1‑251 (MQ=255)                                                                                       aagCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGtttt                                                                                                                              >  2:288378/1‑251 (MQ=255)                                                                                                  cgtcgtcTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTc                                                                                                                                                                                                             <  2:354175/163‑1 (MQ=255)                                                                                                  cgtcgtcTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTc                                                                                                                                                                                                             >  1:354175/1‑163 (MQ=255)                                                                                                                tATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCaa                                                                                                                                       >  1:427766/1‑217 (MQ=255)                                                                                                                       cAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTGGCGATGtttttt                                                                                              <  1:368457/251‑1 (MQ=255)                                                                                                                                 aCCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGcac                                                                                    <  2:89850/251‑1 (MQ=255)                                                                                                                                                    tGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACt                                                                 >  1:374473/1‑251 (MQ=255)                                                                                                                                                       gTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGGAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTGCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTcac                                                              <  1:159501/251‑1 (MQ=255)                                                                                                                                                            aGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTg                                                         <  2:263765/251‑1 (MQ=255)                                                                                                                                                                 aTCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCTGCATTTTGCTTCCCCGCAAct                                                                                                                                            <  2:404734/164‑2 (MQ=255)                                                                                                                                                                 aTCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAct                                                                                                                                            >  1:404734/1‑163 (MQ=255)                                                                                                                                                                    aaTGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATTCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATGTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATaaa                                                 <  1:56867/251‑1 (MQ=255)                                                                                                                                                                            aGTGACAACCTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCg                                                                                                                                                                 >  2:420683/1‑132 (MQ=255)                                                                                                                                                                            aGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCg                                                                                                                                                                 <  1:420683/132‑1 (MQ=255)                                                                                                                                                                                          cgattgAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTActttct                           <  1:58230/246‑1 (MQ=255)                                                                                                                                                                                                              attgaGCAGATGCGCGAGCAGATTCAGAATATTGAATAACGCGTAACACTAAGTCTTACTTGTCAGCCTTCACAAGTGCCGC‑CCACTCAAACAGAGCGGCATTTTGCTTCCCCGCAAC‑TTCAATGCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTTAGATACTCACAACTGTATATAAATACAGGTACAGAATTACTTCCTGTGCAATCAATATCCCATgg       <  2:389470/251‑1 (MQ=255)                                                                                                                                                                                                                   gCAGATGCTCGAGCAGATTCAGAATATTGAGGAACTCGTAACACTAATTCTTACTTTTCAGCCTTCACAAATGCCGC‑CCACTCAAACAGAGCGGCATTTTTCTTCCCCGCAAC‑TTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTGTTTTTGCCCAGCCCACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATGTACTTTCTTTGCAATCGATATCACATGGAGTgg  <  1:141969/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                               GCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAATGCCGC‑C‑ACTCAAACAGAGCGGCATTTTTCTTCCCCGCAACATTCAATTCTGTTTTGCGTGCCTGCTCCAGATTTTGCGATGTTTTTTTGCCCAGCACACTGAGAACGTGAGATACTCACAACTGTATATAAATACAGTTACAGATTTACTTTCTTTGCAATTGATATCACATGGAGTGG  >  NC_000913/3817296‑3817756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑