Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,817,332	T→C	D219D (GAT→GAC)	yicC →	putative RNase adaptor protein YicC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,817,332	0	T	C	97.1%	89.1 / ‑6.0	34	D219D (GAT→GAC)	yicC	putative RNase adaptor protein YicC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (11/22);  minor base A (0/1);  total (11/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG‑TCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAA  >  NC_000913/3817086‑3817576                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gCTGAACGACTGTATTGTCGCGCGCGAAACCGAAGGCCAGGCACTGAAAGCATTGATCGAGCAGCGGCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑GCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGcc                                                                                                                                                                                                                                                  <  2:361613/251‑1 (MQ=255)    gaactacgttATTGTCGGGGGCGAAACCAAAGGTCGGGCACGGAAAGCATGGATCGATCAGCGGCTGAAAGTCGGCCCCGCCTAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGTGAAGCTGGAAGATGCGCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTTGTTCGGCTGGCACAACTAATGGACGTTGCCGAAGAACTGGGCCGCCTc                                                                                                                                                                                                                                                <  2:275911/242‑1 (MQ=255)             tATTGTCGCGGGCGAACCCGAAGGTCGGGCATTGAAAGCATTGACCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGGCCGCCTCGAAGCGCAt                                                                                                                                                                                                                                       <  2:52493/250‑1 (MQ=255)                      gcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAag                                                                                                                                                                                                                                >  2:126338/1‑248 (MQ=255)                       cgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTAACAGTCCGCTCCCATATGCCGGAAATCCTGCACTGGCAGCGTGAACGCTTGG‑TCGCGTAGCTGGAAGATGCTCCGGTTCAGTTGGACAACAACCGACTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTAGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGAc                                                                                                                                                                                                                            >  2:141969/1‑251 (MQ=255)                           aaaCCGAAGGTCAGGCACTGAAAGAAGTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGGCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTGGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTac                                                                                                                                                                                                                        <  1:159869/251‑1 (MQ=255)                                 aaGGTCAGGAACTGAAAGCATTGATCGTGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCAATATGCCGGAAATCCTGCTATGGCAGAGTGAACGCTTGA‑TAGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCAGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGACGAACTGGACCGCCTCGAATCGCATGTTAAAGAGTCGTACAACAtt                                                                                                                                                                                                                  >  2:2739/1‑251 (MQ=255)                                              aaaaGCATTGATCGAGCAGCGCCTGGAAGGGGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACG‑CTGGGTCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCGGGAGCAGGAGTGGGTTCTGCTGGCACAACGAATTGACGGTGCCGAAGAACTGGACCGCCTCGAAGCGCATGGTAAAGAGACGTACAACATTCTGAAGAAAAaag                                                                                                                                                                                                     <  1:365611/250‑1 (MQ=255)                                                 aGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAaaga                                                                                                                                                                                                    >  2:165763/1‑249 (MQ=255)                                                       gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTg                                                                                                                                                                                            >  2:93456/1‑251 (MQ=255)                                                       gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGGACAACATTCTGAAGAAAAAAGAAGCCGTTg                                                                                                                                                                                            >  1:247497/1‑251 (MQ=255)                                                        aTCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTgg                                                                                                                                                                                           >  2:430751/1‑251 (MQ=255)                                                          cgatcagcGTCTGGAAGGCGTCCCCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCGGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGgtc                                                                                                                                                                                         <  2:364683/247‑1 (MQ=255)                                                                       gAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACGGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGactt                                                                                                                                                                            <  1:101866/251‑4 (MQ=255)                                                                        aaGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGActtt                                                                                                                                                                           >  1:412741/1‑251 (MQ=255)                                                                        aaGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCGGCAATGGCAGCGTGACCGCTTGG‑TCGCGAGGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGGGTTGGTTCTGCTGGCACAACGAATGGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGActtt                                                                                                                                                                           <  2:225076/251‑1 (MQ=255)                                                                         aGGCGTCACCGCCGAAGTGGTCAAAGGCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCGGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTa                                                                                                                                                                          <  1:21702/251‑1 (MQ=255)                                                                               cACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTatgatg                                                                                                                                                                     <  1:83201/250‑1 (MQ=255)                                                                                           ggTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAGTTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAAcc                                                                                                                                                        <  1:68698/251‑1 (MQ=255)                                                                                                                                       gtgaacgcttGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTg                                                                                                            <  2:76756/242‑1 (MQ=255)                                                                                                                                         gaacgcttGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTGGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGa                                                                                                           <  2:152784/243‑1 (MQ=255)                                                                                                                                                             tGGAAGATGCTCAGGTTCAGTTGGAAAACAACAGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGAACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGcgattgagatg                                                                                       >  1:156833/1‑241 (MQ=255)                                                                                                                                                                  gATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTAGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAgt                                                                                  <  1:126338/251‑1 (MQ=255)                                                                                                                                                                    tGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAgggt                                                                                >  1:392033/1‑248 (MQ=255)                                                                                                                                                                        caggttcatttGGAAAACAACCGTCGGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGttgat                                                                            <  1:314783/241‑1 (MQ=255)                                                                                                                                                                                   ggAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATgc                                                                 <  2:412741/251‑1 (MQ=255)                                                                                                                                                                                                       aggagttgggtCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGCAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACAGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAACTGACAAACTCCGAGATTGAGCTGAAAGTGTTGATTGCGCAGATGCGCGAGCAGATTCAGaatatt                                             >  2:393276/11‑247 (MQ=255)                                                                                                                                                                                                         gagtgggtgctgctgGCACAACGAATGGACTTTGCCGAAGATCTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGaatattga                                           <  2:355443/242‑7 (MQ=255)                                                                                                                                                                                                           gttGGTTCTGGTGGCCCAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGaatattgagt                                         <  2:247497/249‑9 (MQ=255)                                                                                                                                                                                                                    gctgTCACAACGATTGGACGTTGCCGAATAACTGGACCGCCGCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAGCCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTaa                                <  1:186061/251‑1 (MQ=255)                                                                                                                                                                                                                          acaACGAATTGACGTTGCCGAAGAACGGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATAGTGAGTAACTCGTaatactaa                          <  1:317455/251‑7 (MQ=255)                                                                                                                                                                                                                                         tGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTcag            <  2:292365/250‑3 (MQ=255)                                                                                                                                                                                                                                              aagaaCTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCTTca      <  1:54922/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aCTGGAACGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAATATTGAGTAACTCGTAACACTAATTCTTACTTTTCAGCCGTCACaaa  <  2:82895/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG‑TCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGCGCGAGCAGATTCAGAACATCGAATAACTCGTAACGCCAATTCTTACTTTTCCGCCTTCACAAA  >  NC_000913/3817086‑3817576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑