Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,817,257	G→T	V194V (GTG→GTT)	yicC →	putative RNase adaptor protein YicC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,817,257	0	G	T	100.0%	142.3 / NA	42	V194V (GTG→GTT)	yicC	putative RNase adaptor protein YicC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/25);  total (17/25)

CAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGGA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG‑TCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGT  >  NC_000913/3817030‑3817498                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  cAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTgg                                                                                                                                                                                                                             >  1:309861/1‑250 (MQ=255)  aGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTgg                                                                                                                                                                                                                             >  1:52493/1‑249 (MQ=255)   ggAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑TGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGc                                                                                                                                                                                                                          <  1:38142/251‑1 (MQ=255)         ggATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCGGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGagt                                                                                                                                                                                                                    <  2:108216/251‑3 (MQ=255)               ggACGCCAGTGCCGCTGAAATGCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAAGGGCAGCGTGAACGCTTGA‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGagttggtt                                                                                                                                                                                                               <  2:125693/249‑8 (MQ=255)                         gCCGCTGAAATGCTCGCGGCGCTGGATGGTACGC‑GGAGCTCCTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCAGTGTTCGCGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCGCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGcaca                                                                                                                                                                                                    <  2:99004/251‑1 (MQ=255)                          ccGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGagtt                                                                                                                                                                                                                   <  2:368359/235‑4 (MQ=255)                          ccGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGagtt                                                                                                                                                                                                                   >  1:368359/1‑232 (MQ=255)                               gAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCCGCGTCTGGAAGGCGTAACCGCCGAAGTGGTCAAAGTCAGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAAAGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAAACGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAAt                                                                                                                                                                                              >  1:194897/1‑251 (MQ=255)                                aacgtctcGCTGGGCTGGATGGTACGCTGAA‑CGACTTTATGGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTTATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAAtt                                                                                                                                                                                             <  2:141893/247‑1 (MQ=255)                                aaaTTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAACGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAACCAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAAtt                                                                                                                                                                                             >  1:389470/1‑251 (MQ=255)                                       cgcgGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGt                                                                                                                                                                                        <  1:257778/249‑1 (MQ=255)                                         cggcgCTGGATGGGACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGTATTTGGTTCTGCTGGCACAACGAATTGACGTTGcc                                                                                                                                                                                    <  1:119670/251‑1 (MQ=255)                                          ggcgCTGGATGGTACGCTGAA‑CGACTTTAGTGTCGCGCGCGAAACCGAAGGCCAGGCACTGAAAGCATTGATCGAGCCGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCg                                                                                                                                                                                   <  2:358158/251‑1 (MQ=255)                                                  aTGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCTGACACTGAAAGCATTGATCGAGCAGAGTCTGGAAGGCGTCACCGCCGACGTGGTCAAAGTCCGCTCCCCTATGCCGGCACTACTGCAATGGAAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCAGAAGAACTg                                                                                                                                                                           >  1:293051/1‑251 (MQ=255)                                                   ggttACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTgg                                                                                                                                                                          <  1:412192/248‑1 (MQ=255)                                                         gCTGAA‑CGACTGTATTGTCGCGCGCGAAACCGAAGGCCAGGCACTGAAAGCATTGATCGAGCAGCGGCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑GCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGcc                                                                                                                                                                    <  2:361613/251‑1 (MQ=255)                                                             gaactacgttATTGTCGGGGGCGAAACCAAAGGTCGGGCACGGAAAGCATGGATCGATCAGCGGCTGAAAGTCGGCCCCGCCTAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGTGAAGCTGGAAGATGCGCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTTGTTCGGCTGGCACAACTAATGGACGTTGCCGAAGAACTGGGCCGCCTc                                                                                                                                                                  <  2:275911/242‑1 (MQ=255)                                                                    tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTg                                                                                                                                                                                            >  1:48791/1‑217 (MQ=255)                                                                    tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTg                                                                                                                                                                                            <  2:48791/217‑1 (MQ=255)                                                                      tATTGTCGCGGGCGAACCCGAAGGTCGGGCATTGAAAGCATTGACCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGGCCGCCTCGAAGCGCAt                                                                                                                                                         <  2:52493/250‑1 (MQ=255)                                                                               gcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAag                                                                                                                                                  >  2:126338/1‑248 (MQ=255)                                                                                cgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTAACAGTCCGCTCCCATATGCCGGAAATCCTGCACTGGCAGCGTGAACGCTTGG‑TCGCGTAGCTGGAAGATGCTCCGGTTCAGTTGGACAACAACCGACTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTAGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGAc                                                                                                                                              >  2:141969/1‑251 (MQ=255)                                                                                    aaaCCGAAGGTCAGGCACTGAAAGAAGTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGGCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTGGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTac                                                                                                                                          <  1:159869/251‑1 (MQ=255)                                                                                          aaGGTCAGGAACTGAAAGCATTGATCGTGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCAATATGCCGGAAATCCTGCTATGGCAGAGTGAACGCTTGA‑TAGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCAGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGACGAACTGGACCGCCTCGAATCGCATGTTAAAGAGTCGTACAACAtt                                                                                                                                    >  2:2739/1‑251 (MQ=255)                                                                                                       aaaaGCATTGATCGAGCAGCGCCTGGAAGGGGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACG‑CTGGGTCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCGGGAGCAGGAGTGGGTTCTGCTGGCACAACGAATTGACGGTGCCGAAGAACTGGACCGCCTCGAAGCGCATGGTAAAGAGACGTACAACATTCTGAAGAAAAaag                                                                                                                       <  1:365611/250‑1 (MQ=255)                                                                                                          aGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAaaga                                                                                                                      >  2:165763/1‑249 (MQ=255)                                                                                                                gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTg                                                                                                              >  2:93456/1‑251 (MQ=255)                                                                                                                gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGGACAACATTCTGAAGAAAAAAGAAGCCGTTg                                                                                                              >  1:247497/1‑251 (MQ=255)                                                                                                                 aTCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTgg                                                                                                             >  2:430751/1‑251 (MQ=255)                                                                                                                   cgatcagcGTCTGGAAGGCGTCCCCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCGGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGgtc                                                                                                           <  2:364683/247‑1 (MQ=255)                                                                                                                                gAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACGGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGactt                                                                                              <  1:101866/251‑4 (MQ=255)                                                                                                                                 aaGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGActtt                                                                                             >  1:412741/1‑251 (MQ=255)                                                                                                                                 aaGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCGGCAATGGCAGCGTGACCGCTTGG‑TCGCGAGGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGGGTTGGTTCTGCTGGCACAACGAATGGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGActtt                                                                                             <  2:225076/251‑1 (MQ=255)                                                                                                                                  aGGCGTCACCGCCGAAGTGGTCAAAGGCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCGGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTa                                                                                            <  1:21702/251‑1 (MQ=255)                                                                                                                                        cACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTatgatg                                                                                       <  1:83201/250‑1 (MQ=255)                                                                                                                                                    ggTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAGTTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAAcc                                                                          <  1:68698/251‑1 (MQ=255)                                                                                                                                                                                                gtgaacgcttGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTg                              <  2:76756/242‑1 (MQ=255)                                                                                                                                                                                                  gaacgcttGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTGGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGa                             <  2:152784/243‑1 (MQ=255)                                                                                                                                                                                                                      tGGAAGATGCTCAGGTTCAGTTGGAAAACAACAGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGAACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGcgattgagatg         >  1:156833/1‑241 (MQ=255)                                                                                                                                                                                                                           gATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTAGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAgt    <  1:126338/251‑1 (MQ=255)                                                                                                                                                                                                                             tGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAgggt  >  1:392033/1‑248 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  CAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGGA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG‑TCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGT  >  NC_000913/3817030‑3817498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑