Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,816,406	C→A	P48P (CCG→CCT)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,816,406	0	C	A	100.0%	211.6 / NA	59	P48P (CCG→CCT)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/38);  total (21/38)

AATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCGGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTG‑AAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGACAAGGCATCG  >  NC_000913/3816160‑3816630                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  aaTCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCa                                                                                                                                                                                                                               <  1:86489/251‑1 (MQ=255)     gaagcagTACGCGTGCCACCATCCGCCTGACGCACGTCGCAGTCCAGCGTACTGGTGCACTCACCCAGCGCTTTCAAATATACTGCCGCGCGAATTGCACGGGCGATCAGACGCTGGATTTACCTTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGAGGCAGCATACCGGCCTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGCAGCGAGGCCCGcc                                                                                                                                                                                                                           >  2:186850/7‑251 (MQ=255)         cgGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGCGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCcttct                                                                                                                                                                                                                       >  1:29974/1‑251 (MQ=255)                   gCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATagag                                                                                                                                                                                                              <  1:292008/250‑1 (MQ=255)                     caccaTCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCg                                                                                                                                                                                                           <  1:428132/251‑1 (MQ=255)                                 gAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACacaatac                                                                                                                                                                                                <  2:254615/250‑4 (MQ=255)                                          cGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCg                                                                                                                                                                                      <  1:403903/251‑1 (MQ=255)                                             aGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGcc                                                                                                                                                                                    >  1:306125/1‑250 (MQ=255)                                                 cAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTAAACAATACTTTGTTATCGCCAAAtt                                                                                                                                                                               >  2:240380/1‑251 (MQ=255)                                                     gTAATGGTGAAATCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTATTCAATAGAGGCGGTACACAATACTGTGGGATCGCCAAATTctac                                                                                                                                                                           >  1:88851/1‑248 (MQ=255)                                                       aaTGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGAcc                                                                                                                                                                          <  1:200923/250‑1 (MQ=255)                                                           gTGACCTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTAAACAATACTTTGGTATCGCCAAAttttcccagccc                                                                                                                                                                     >  2:35267/1‑241 (MQ=255)                                                               aCTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGTCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTGTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAg                                                                                                                                                                 <  2:136764/251‑1 (MQ=255)                                                                             ttCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGtt                                                                                                                                                    <  1:222902/250‑1 (MQ=255)                                                                               cAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATGTCCATTGTGCGGCCACCCGGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGtttt                                                                                                                                                  <  2:139371/250‑1 (MQ=255)                                                                                      aCTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTTCCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTCCTGCATGTTTTGTATAg                                                                                                                                            <  2:325160/249‑1 (MQ=255)                                                                                      aCTGCCGAGCGAAGTGCACGGGCGACCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACAGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATTCTTTGGTATCGCCAAATTCGACCATCACCGAGCCTTCTGCATGTTTTGTATAg                                                                                                                                            <  2:7044/249‑1 (MQ=255)                                                                                        tGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACCCAATACTTTGGTATCGCCAAATTCGACCCACCCCGCGCCTTCTGCCTGTTTTTTATAGttcc                                                                                                                                        >  2:30754/1‑249 (MQ=255)                                                                                         gCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTGCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGTTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTGGTATAGTTAc                                                                                                                                        <  2:164877/250‑1 (MQ=255)                                                                                                gAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTAACCAATACTTTGTTATCTCCAAAATCGACCCGCACCCAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAgg                                                                                                                                >  2:80745/1‑251 (MQ=255)                                                                                                      cACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCTCCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACg                                                                                                                          <  2:243691/251‑1 (MQ=255)                                                                                                       aCGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTTGACCCGCACCGAGCCCTCTGCATGTTTTGGATTGTTTCGAGTAAGGGTAACgg                                                                                                                         >  2:120068/1‑251 (MQ=255)                                                                                                        cGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACggg                                                                                                                        >  1:299716/1‑251 (MQ=255)                                                                                                                   aCGCTGGATTTCCATTGTGCGTCCACCCTGCTTCCCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGat                                                                                                             <  2:289358/251‑1 (MQ=255)                                                                                                                   aCGCTGGATTTCCATTGTGCGTCCACCCTGCTGACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGat                                                                                                             <  1:402223/251‑1 (MQ=255)                                                                                                                           tttCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCATGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGGAACGGGACGCACCTGATTATTGCt                                                                                                      >  1:182864/1‑250 (MQ=255)                                                                                                                            ttCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTGTGTATAGTTACGAGTCAGGGTAACGGGACGCGCCTGATTAGTGCTa                                                                                                     <  2:190173/250‑1 (MQ=255)                                                                                                                            ttCCATTGTGCGGCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACGCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCATCACCGAGCCTTCTTCATGTTTTGTATAGTTACGAGTCAGGGTAACGGTACGCATCTGATGAGTGCTa                                                                                                     <  2:399401/250‑1 (MQ=255)                                                                                                                             tCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAggg                                                                                                                               <  1:133278/223‑1 (MQ=255)                                                                                                                              ccATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACgg                                                                                                  <  1:90046/251‑1 (MQ=255)                                                                                                                                  tgtgCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCct                                                                                               <  1:301654/250‑1 (MQ=255)                                                                                                                                  tgtgCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCct                                                                                               <  1:49218/250‑1 (MQ=255)                                                                                                                                  tgtgCGTCCACCCGGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCGACGGCct                                                                                               <  2:42037/250‑1 (MQ=255)                                                                                                                                       gTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGGATCGCCAAATTCGGCCAGCACCGGGCCTTCTGCATGTTTTGTATAGTTACGAGTAAGGGTAAAGGGAAGCACCTGATTATTGCTACGGCCTGCTggg                                                                                         >  1:129834/1‑250 (MQ=255)                                                                                                                                         ccACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGGATCGCCAAATTTGACCAGCACCCAGCCTTCTGCCTGTTTTGTATAGTTACGGGTCCGGGTAAAGGGCCGCACCTGATTATTGCTACGGCctgcctggcg                                                                                       >  1:17819/1‑245 (MQ=255)                                                                                                                                             ccTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCata                                                                                   <  1:75994/251‑1 (MQ=255)                                                                                                                                             ccTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCata                                                                                   <  2:176700/251‑1 (MQ=255)                                                                                                                                                gCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCTCCTGATTATTGCTACGGCCTGCTGGACGCATAtt                                                                                 <  1:372273/250‑1 (MQ=255)                                                                                                                                                gCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCatat                                                                                  <  1:35267/249‑1 (MQ=255)                                                                                                                                                    aCCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGaaa                                                                             <  1:134325/250‑1 (MQ=255)                                                                                                                                                            ctgcTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACATTGGTATCGACAAATTCGACCAGCACCGAGCCTTCTGGATGTTTTGTATAGTTACGAGTCAGGGTAACGGGGCGGACCTGATTATTGCTACGGCCTGCTGGACGCAAATTGAAATCTCCGGCt                                                                    >  1:20466/3‑251 (MQ=255)                                                                                                                                                                ttCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTG‑a                                                                >  1:331027/1‑250 (MQ=255)                                                                                                                                                                   aCGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTCCCCAAAACTTTGGTATTGCCCAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGGGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGATGCATATTGAAATCTCCGGCTTG‑AAAc                                                             >  1:148260/1‑250 (MQ=255)                                                                                                                                                                               ggTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATGATTGCTACGGCCTGCTGGACGCAGATTGAAATCTCCGGCTTG‑AAACAAATGTGGCTgcg                                                <  2:29974/251‑1 (MQ=255)                                                                                                                                                                                     ggTAGAACGCGGCAGCATACCGGACTCTGCGGTGATCCAGCCATGGCCCTGTCCTTTCAGGAAGCGAGGCTCGCCTTCTTCAATAGAGGCTGTACACACTACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTCCGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGAATATTGAAATCTCCGGCTTG‑AAACAAATGTGGCGGCGCATtat                                          <  2:235132/251‑1 (MQ=255)                                                                                                                                                                                         gAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTG‑AAACAAATGTGGCTGCGCATTATACgg                                      <  2:51288/251‑1 (MQ=255)                                                                                                                                                                                         gAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCCAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCCCCTGATTATTGCTACGGCCTGCTGGACGCATATTGGAATCTCCGGCTTG‑AAACAAATGTGGCTGCGCATTCTACgg                                      >  1:184155/1‑251 (MQ=255)                                                                                                                                                                                          aaCGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGGGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCCCCTGATTATTGCTACGGCCTGCTGGGCGCATATTGAAATCTCCGGCTTGTAAACaat                                                          >  2:72180/1‑230 (MQ=255)                                                                                                                                                                                          aaCGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTG‑AAACAAAt                                                         <  1:72180/231‑1 (MQ=255)                                                                                                                                                                                          aaCGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTG‑AAACAAATGTGGCTGCGCATTATACGGa                                     <  1:278577/251‑1 (MQ=255)                                                                                                                                                                                                     ataccGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGATGCATATTGAAATCTCCGGCTTG‑AAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGt                          <  2:148260/248‑1 (MQ=255)                                                                                                                                                                                                       accGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTG‑AAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTa                        <  2:331027/250‑1 (MQ=255)                                                                                                                                                                                                          gTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACCATAATTTTGTATCGCCAAATTCGACCAGCACCGGGCCTTCTGCATGTTTTGTCTAGTTACGAGTCAGGGTAAAGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTG‑AAACAAATTTGGCTGCGCATTATACGGACTTCCGGCGGTTAttt                     >  2:416108/1‑250 (MQ=255)                                                                                                                                                                                                            aCTCGGCGGTGATCCAGCCCTGGCCCTGCCCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTGCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCGACGTCCTGCTGGACGCATATTGAAATCGCCGGCTGG‑TAACAAAGGTGGCTTTGCATTATACGGACTTCCGGCGGGTATTCCt                   <  2:396543/251‑1 (MQ=255)                                                                                                                                                                                                                  cGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACACTACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCCTGTTTTGTATAGTTACGAGTCAGGGGAACGGGACGCACCTGATTATTTCTACGGCCTGCTGGACGCATATTGCAATCTCCGGCTTG‑AAACAAATGTGGCTGCGCATTATACGGACTTCccgcgggtttttctattcct             >  2:142526/1‑237 (MQ=255)                                                                                                                                                                                                                    gTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCATGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTG‑AAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGAc           <  1:69555/251‑1 (MQ=255)                                                                                                                                                                                                                       aTCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGCGCCTTTTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGa                                                                               >  1:359940/1‑181 (MQ=255)                                                                                                                                                                                                                       aTCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGa                                                                               <  2:359940/181‑1 (MQ=255)                                                                                                                                                                                                                             ccctggccctgACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTG‑AAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGACAAGGCATCg  <  1:207745/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  AATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCGGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTG‑AAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGACAAGGCATCG  >  NC_000913/3816160‑3816630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑