Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,816,259	A→T	A97A (GCT→GCA)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,816,259	0	A	T	98.5%	217.3 / NA	68	A97A (GCT→GCA)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (37/28);  minor base C (1/0);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCGGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGG  >  NC_000913/3816017‑3816505                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       aCGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGCAGTGCACGGGc                                                                                                                                                                                                                                                >  2:200923/1‑251 (MQ=255)    tATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGAt                                                                                                                                                                                                                                             >  1:243691/1‑251 (MQ=255)          aGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAACCCGCGGCTACCATCCCTTTCATTGGATTGGTTGTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGatg                                                                                                                                                                                                                                       <  2:302192/251‑3 (MQ=255)            aTCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCt                                                                                                                                                                                                                                     <  1:396126/251‑1 (MQ=255)                      gCTTCACCGTTTACAATACAGACAGAAACCCCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCAGTTTTCCAACAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGCAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCAt                                                                                                                                                                                                                           >  1:159969/1‑251 (MQ=255)                       cTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCACATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCAtt                                                                                                                                                                                                                          >  1:151756/1‑251 (MQ=255)                          caccGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATtgtg                                                                                                                                                                                                                       <  1:184819/249‑1 (MQ=255)                           accGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTATAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGAGGTACGCGTGCCACCATCGGCCTGACGCACGTCGCAGTCCAGAGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCCAAGTGCACGGTCGAACAGCCGCTGGTTTTCCAttacgc                                                                                                                                                                                                                      >  1:430867/2‑247 (MQ=255)                                 tacaATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCCGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCAc                                                                                                                                                                                                                >  2:352252/2‑251 (MQ=255)                                  acaATACCGACAGAAACCGCGGTTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCATACGCTGGATTTCCATTGTGCGTCCAcc                                                                                                                                                                                                               >  1:139371/1‑251 (MQ=255)                                  acaATACCGACAGAAACCGAGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGCGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCAcc                                                                                                                                                                                                               >  2:49218/1‑251 (MQ=255)                                   caATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCCCGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCACATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCAccc                                                                                                                                                                                                              >  2:193784/1‑251 (MQ=255)                                     ataccGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGGTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTg                                                                                                                                                                                                            <  2:90908/248‑1 (MQ=255)                                          gACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTAAGCTTGCCGTTTTCCACCAGCTTATGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAAATCACACAGCGCTTTCAAATCTACTGCCGCGAGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCttc                                                                                                                                                                                                        >  1:279953/1‑249 (MQ=255)                                           aCAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTAcc                                                                                                                                                                                                      <  1:85361/251‑1 (MQ=255)                                           aCAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTAcc                                                                                                                                                                                                      >  1:51288/1‑251 (MQ=255)                                             aGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCTCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCtt                                                                                                                                                                                                    <  2:167034/251‑1 (MQ=255)                                              gAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCttt                                                                                                                                                                                                   >  2:301654/1‑251 (MQ=255)                                                      ggCTACCATCCCTTTCATTGGATGGGTTTTCAGCTTTCCGTTTTCCACCAGCATCTGTAGCCCATATGCCAGCGCCACACAGGCACCCGTAATCGCAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCTAGCGTAATGGAGAACTCACCCAGCGCTTTCAAATCTACCGCTGCACGAGGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTACACCCTGCTTACCTTTCGCTGCtt                                                                                                                                                                                           >  2:130871/1‑251 (MQ=255)                                                       gCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCACGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTc                                                                                                                                                                                          >  1:399401/1‑251 (MQ=255)                                                          aCCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTTTGTAGCGCATCTGCCAGCGCCACTCAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGCAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACg                                                                                                                                                                                       >  2:59546/1‑251 (MQ=255)                                                          aCCATCCCTTTCATTGGATTGGTGTTCAGCGTGCCGTTTTCCTCCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGGGCCACCATCAGCCTGAAGCACGTCGCAGTCCGGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCCCGAAGGGCACGTGCGATCAGACGCTGGATGGCCATTGTGCGTCCACCCTGCTTTCCTTTCGCTGCATCACg                                                                                                                                                                                       <  2:431867/251‑1 (MQ=255)                                                            cATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACg                                                                                                                                                                                       >  2:134325/1‑249 (MQ=255)                                                             aTCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGc                                                                                                                                                                                    >  1:208247/1‑251 (MQ=255)                                                                   atcattgcaTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACCCGCGCCACCATAAGCCTGAAGCACGTCGCAGTCCCGCGTATTGGTGAACTCAACCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCGTTCTGCGTCCACACTGCTTACCTTCCGCTGCTCCACGCGCGTTACg                                                                                                                                                                              >  2:275140/9‑251 (MQ=255)                                                                     cattggATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACgg                                                                                                                                                                             <  1:356388/246‑1 (MQ=255)                                                                     cattggATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGCATCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACTTCGCAGTCCCGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCTCACGTAGTGCACGGGCGCTCAGACGCTGGCTTTCCATTGGGCGTCCACCCTGCTTACCTTTCGCTGCTTCCCGATCGTTACggg                                                                                                                                                                            >  2:232991/5‑251 (MQ=255)                                                                      attggATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGAATCTGCCAGCGCCAAACATGCACACGTAATCGAAGCAGTACGCGTGCCACCATCCGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACATTTCGCTGCTACACGAGCGTTACGGgt                                                                                                                                                                           >  2:182279/4‑251 (MQ=255)                                                                         ggATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGtgtg                                                                                                                                                                        <  1:67286/251‑1 (MQ=255)                                                                          gATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTgg                                                                                                                                                                       >  1:289358/1‑251 (MQ=255)                                                                            ttGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGt                                                                                                                                                                     >  1:42037/1‑251 (MQ=255)                                                                              ggTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGCAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCCGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGt                                                                                                                                                                     >  2:278577/1‑249 (MQ=255)                                                                                  ttCAGCTTGCCGTTTTCCACCAGCTGCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTACTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAAc                                                                                                                                                                <  2:419077/250‑1 (MQ=255)                                                                                    cAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGAt                                                                                                                                                                                                                                             >  1:188296/1‑171 (MQ=255)                                                                                    cAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGAt                                                                                                                                                                                                                                             <  2:188296/171‑1 (MQ=255)                                                                                    cAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACgcg                                                                                                                                                             >  1:176700/1‑249 (MQ=255)                                                                                     aGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACgcgg                                                                                                                                                            >  1:127309/1‑248 (MQ=255)                                                                                      gcgtGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGCAGCTGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTTAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCGCCCTGCTTACCGTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGatcgcggc                                                                                                                                                           <  2:210769/248‑8 (MQ=255)                                                                                         tGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGgcagc                                                                                                                                                        >  1:190173/1‑251 (MQ=255)                                                                                                                               gcCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGAcc                                                                                                                  >  2:428132/1‑251 (MQ=255)                                                                                                                                 cacacAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCtt                                                                                                                >  1:79537/5‑251 (MQ=255)                                                                                                                                  acacAGGCACCCGTAATCGAAGCGGTACGCGGTCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCGGGCCCTGACCttt                                                                                                               <  2:208247/248‑1 (MQ=255)                                                                                                                                      aGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAgg                                                                                                           >  1:140711/1‑251 (MQ=255)                                                                                                                                       ggCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGa                                                                                                          <  1:354385/251‑1 (MQ=255)                                                                                                                                        gCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGaa                                                                                                         <  1:360591/251‑1 (MQ=255)                                                                                                                                           cccGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCATTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCg                                                                                                                                         <  2:251066/216‑1 (MQ=255)                                                                                                                                           cccGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCg                                                                                                                                         >  1:251066/1‑216 (MQ=255)                                                                                                                                           cccGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAgcg                                                                                                      <  2:151756/251‑1 (MQ=255)                                                                                                                                                aaTCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCa                                                                                                 <  1:86489/251‑1 (MQ=255)                                                                                                                                                    gaagcagTACGCGTGCCACCATCCGCCTGACGCACGTCGCAGTCCAGCGTACTGGTGCACTCACCCAGCGCTTTCAAATATACTGCCGCGCGAATTGCACGGGCGATCAGACGCTGGATTTACCTTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGAGGCAGCATACCGGCCTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGCAGCGAGGCCCGcc                                                                                             >  2:186850/7‑251 (MQ=255)                                                                                                                                                        cgGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGCGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCcttct                                                                                         >  1:29974/1‑251 (MQ=255)                                                                                                                                                                  gCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATagag                                                                                <  1:292008/250‑1 (MQ=255)                                                                                                                                                                    caccaTCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCg                                                                             <  1:428132/251‑1 (MQ=255)                                                                                                                                                                                gAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACacaatac                                                                  <  2:254615/250‑4 (MQ=255)                                                                                                                                                                                         cGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCg                                                        <  1:403903/251‑1 (MQ=255)                                                                                                                                                                                            aGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGcc                                                      >  1:306125/1‑250 (MQ=255)                                                                                                                                                                                                cAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTAAACAATACTTTGTTATCGCCAAAtt                                                 >  2:240380/1‑251 (MQ=255)                                                                                                                                                                                                    gTAATGGTGAAATCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTATTCAATAGAGGCGGTACACAATACTGTGGGATCGCCAAATTctac                                             >  1:88851/1‑248 (MQ=255)                                                                                                                                                                                                      aaTGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGAcc                                            <  1:200923/250‑1 (MQ=255)                                                                                                                                                                                                          gTGACCTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTAAACAATACTTTGGTATCGCCAAAttttcccagccc                                       >  2:35267/1‑241 (MQ=255)                                                                                                                                                                                                              aCTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGTCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTGTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAg                                   <  2:136764/251‑1 (MQ=255)                                                                                                                                                                                                                            ttCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGtt                      <  1:222902/250‑1 (MQ=255)                                                                                                                                                                                                                              cAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATGTCCATTGTGCGGCCACCCGGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGtttt                    <  2:139371/250‑1 (MQ=255)                                                                                                                                                                                                                                     aCTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTTCCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTCCTGCATGTTTTGTATAg              <  2:325160/249‑1 (MQ=255)                                                                                                                                                                                                                                     aCTGCCGAGCGAAGTGCACGGGCGACCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACAGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATTCTTTGGTATCGCCAAATTCGACCATCACCGAGCCTTCTGCATGTTTTGTATAg              <  2:7044/249‑1 (MQ=255)                                                                                                                                                                                                                                       tGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACCCAATACTTTGGTATCGCCAAATTCGACCCACCCCGCGCCTTCTGCCTGTTTTTTATAGttcc          >  2:30754/1‑249 (MQ=255)                                                                                                                                                                                                                                        gCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTGCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGTTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTGGTATAGTTAc          <  2:164877/250‑1 (MQ=255)                                                                                                                                                                                                                                               gAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTAACCAATACTTTGTTATCTCCAAAATCGACCCGCACCCAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAgg  >  2:80745/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       ACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCGGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGG  >  NC_000913/3816017‑3816505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑