Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,816,055	T→A	G165G (GGA→GGT)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,816,055	0	T	A	98.4%	205.1 / NA	63	G165G (GGA→GGT)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (40/22);  total (40/23)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCC  >  NC_000913/3815811‑3816301                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGAc                                                                                                                                                                                                                                                   >  1:306623/1‑251 (MQ=255)    cTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGAc                                                                                                                                                                                                                                                   >  1:438059/1‑248 (MQ=255)                  tAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTAcc                                                                                                                                                                                                                                   <  1:243955/250‑1 (MQ=255)                               gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCCGCGCCAACAAGGTCAGTCGCTCTTCCTGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTCCCATCCCTTTCAttgg                                                                                                                                                                                                                     >  1:288126/1‑248 (MQ=255)                                    cgccgcCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCattggattgg                                                                                                                                                                                                                <  2:82711/251‑1 (MQ=255)                                       cgccTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTAGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGttt                                                                                                                                                                                                             >  1:261527/1‑251 (MQ=255)                                              tGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCCTTGGCTTGGTTTTCAGCtt                                                                                                                                                                                                      >  2:360591/1‑251 (MQ=255)                                                   cGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGt                                                                                                                                                                                                 >  2:86489/1‑251 (MQ=255)                                                   cGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGt                                                                                                                                                                                                 >  2:184819/1‑251 (MQ=255)                                                      tACAATGGATTCGATTCCACC‑TCGGGCCAGCGCCACCAAGGCGAGAAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCACCGTATTCCAGATCGCAAATTCCTTCACCGTTTACAATACCGACAGAAACCGCGGCTCCCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGtttt                                                                                                                                                                                              >  2:67286/1‑251 (MQ=255)                                                                cgagtccc‑cc‑TCGGTCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCGTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCGCCGTTTACAATTCCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTTCACCAGCtt                                                                                                                                                                                    <  2:227980/244‑1 (MQ=255)                                                                   ttccc‑cc‑TCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGt                                                                                                                                                                                >  2:396126/4‑251 (MQ=255)                                                                          c‑TCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAgcgc                                                                                                                                                                           >  1:396543/1‑250 (MQ=255)                                                                               ggCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCtg                                                                                                                                                                      <  1:122627/251‑2 (MQ=255)                                                                                  aagcgcaaacaaGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAACTTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCCGCTTCTGTAGCGCATCtgca                                                                                                                                                                    >  2:69555/8‑247 (MQ=255)                                                                                   agcgCCAACAAGGTGAGTAGCTCATCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCGGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGCCGGTCTCTGCGGCGGAGTCGTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACAGACAGAAACCGCGGCTACCATCCCTTTAATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTACTGTAgccgctctgcc                                                                                                                                                                    >  2:372273/4‑240 (MQ=255)                                                                                     cgCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCCTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCCGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGAATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAgcc                                                                                                                                                                >  1:83033/2‑250 (MQ=255)                                                                                        caacaaGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGcc                                                                                                                                                              >  1:254615/1‑250 (MQ=255)                                                                                          acaaGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCCTTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCAc                                                                                                                                                            >  2:356388/1‑250 (MQ=255)                                                                                                tGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTAcc                                                                                                                                                                                                                                   >  2:351291/1‑173 (MQ=255)                                                                                                tGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTAcc                                                                                                                                                                                                                                   <  1:351291/173‑1 (MQ=255)                                                                                                 gAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCAc                                                                                                                                                    <  1:262726/251‑1 (MQ=255)                                                                                                   gTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCAccc                                                                                                                                                  <  2:306623/251‑1 (MQ=255)                                                                                                   gTAGCCCTTCATGGGTGAACCGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGCTGCGCACGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCAccc                                                                                                                                                  >  2:222902/1‑251 (MQ=255)                                                                                                     aGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGt                                                                                                                                                <  1:211593/251‑1 (MQ=255)                                                                                                        tctTCATGGGTGAACGGCTCGCCTTCTGCCGTACCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCTCCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAAt                                                                                                                                             >  1:136764/1‑251 (MQ=255)                                                                                                         ctTCATGGGTGACCGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTaa                                                                                                                                              <  2:358316/249‑1 (MQ=255)                                                                                                          ttCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCGACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCg                                                                                                                                           >  1:164877/1‑251 (MQ=255)                                                                                                          ttCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAAt                                                                                                                                             <  1:50134/249‑1 (MQ=255)                                                                                                          ttCATGGGTGAACGGCTCGCCTTCTGCAGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCCCACAGGCACCCggcat                                                                                                                                             >  2:50134/1‑245 (MQ=255)                                                                                                             aTGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGaag                                                                                                                                        <  1:213632/251‑3 (MQ=255)                                                                                                                ggTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCgg                                                                                                                                     >  2:354385/1‑251 (MQ=255)                                                                                                                             ccTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACCATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGaagc                                                                                                                                       >  1:41527/1‑233 (MQ=255)                                                                                                                             ccTTCTGCCGTCCCCGTCACTGCAATGAGGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCCCCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCGGTAGCGCATCTGCCATCGCCACACAGGCACCCGTatgcgaagc                                                                                                                                       <  2:41527/236‑9 (MQ=255)                                                                                                                                                cTTCAATGATGCGCCCGTCTTCGGTCATCCCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCACATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCAAAGCGGTACGCGTGCCACCATCAGCCTGACGCACGTCg                                                                                                     >  2:207745/1‑251 (MQ=255)                                                                                                                                                       gatgCGCCCGTCTTCGGTCATCCCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTGTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCAGCAGCCTGAAGCACGTCGCAGTCCa                                                                                              <  2:438059/251‑1 (MQ=255)                                                                                                                                                         tgCGCCCTTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCTTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAg                                                                                             <  1:29982/250‑1 (MQ=255)                                                                                                                                                            gcCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGt                                                                                          >  2:90046/1‑250 (MQ=255)                                                                                                                                                            gcCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTa                                                                                         <  1:180941/251‑1 (MQ=255)                                                                                                                                                                  cTTCGGTCATCCCTAAGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTAACATCCCTTTCATTGGATTGGTTTTCCGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCCCCATCAGCCCGACGCACGTCCCCGTCCAGCGTAATGGTg                                                                                   >  1:359794/1‑251 (MQ=255)                                                                                                                                                                     cGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAAc                                                                                <  1:32142/251‑1 (MQ=255)                                                                                                                                                                           tcaCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGCTCGCAAATTGCTTCACCGTTTACAATAACGACAGAAACCGCGGCTACCATCCCTTTCCTTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCAccc                                                                          >  1:431867/1‑251 (MQ=255)                                                                                                                                                                             aCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCAccc                                                                          >  1:98349/1‑249 (MQ=255)                                                                                                                                                                                  gTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCTCGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCtt                                                                   <  2:39345/251‑1 (MQ=255)                                                                                                                                                                                          cGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCTACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTTCCACCATCAGCCTGAAGCACGTCGCAGGCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATc                                                            <  2:261527/250‑1 (MQ=255)                                                                                                                                                                                                  gCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACGCACCCAGCGCTTTCAAATCTACTGCcg                                                    <  2:43264/250‑1 (MQ=255)                                                                                                                                                                                                       tgagTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGCAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTCATGGTGAACTCACCCAGCGCTTTCAAATCTCCTGCCGCGCGa                                               >  2:75994/2‑250 (MQ=255)                                                                                                                                                                                                       tgagTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGcca                                                                                                                          >  1:41135/2‑175 (MQ=255)                                                                                                                                                                                                       tgagTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGcca                                                                                                                          <  2:41135/174‑1 (MQ=255)                                                                                                                                                                                                       tgagTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAACGCTTTCAAATCTACTGCCGCGCGa                                               >  1:261153/2‑250 (MQ=255)                                                                                                                                                                                                          gTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAagtg                                           >  2:85361/1‑249 (MQ=255)                                                                                                                                                                                                                 aCGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGCAGTGCACGGGc                                    >  2:200923/1‑251 (MQ=255)                                                                                                                                                                                                                    tATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGAt                                 >  1:243691/1‑251 (MQ=255)                                                                                                                                                                                                                          aGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAACCCGCGGCTACCATCCCTTTCATTGGATTGGTTGTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGatg                           <  2:302192/251‑3 (MQ=255)                                                                                                                                                                                                                            aTCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCt                         <  1:396126/251‑1 (MQ=255)                                                                                                                                                                                                                                      gCTTCACCGTTTACAATACAGACAGAAACCCCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCAGTTTTCCAACAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGCAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCAt               >  1:159969/1‑251 (MQ=255)                                                                                                                                                                                                                                       cTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCACATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCAtt              >  1:151756/1‑251 (MQ=255)                                                                                                                                                                                                                                          caccGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATtgtg           <  1:184819/249‑1 (MQ=255)                                                                                                                                                                                                                                           accGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTATAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGAGGTACGCGTGCCACCATCGGCCTGACGCACGTCGCAGTCCAGAGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCCAAGTGCACGGTCGAACAGCCGCTGGTTTTCCAttacgc          >  1:430867/2‑247 (MQ=255)                                                                                                                                                                                                                                                 tacaATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCCGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCAc    >  2:352252/2‑251 (MQ=255)                                                                                                                                                                                                                                                  acaATACCGACAGAAACCGCGGTTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCATACGCTGGATTTCCATTGTGCGTCCAcc   >  1:139371/1‑251 (MQ=255)                                                                                                                                                                                                                                                  acaATACCGACAGAAACCGAGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGCGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCAcc   >  2:49218/1‑251 (MQ=255)                                                                                                                                                                                                                                                   caATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCCCGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCACATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCAccc  >  2:193784/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       CGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCC  >  NC_000913/3815811‑3816301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑