Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,816,049	C→T	V167V (GTG→GTA)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,816,049	0	C	T	100.0%	206.8 / NA	60	V167V (GTG→GTA)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/23);  total (37/23)

AAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGC  >  NC_000913/3815807‑3816293                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     aaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTAGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAAtac                                                                                                                                                                                                                                               >  1:235132/1‑249 (MQ=255)     cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGAc                                                                                                                                                                                                                                           >  1:306623/1‑251 (MQ=255)        cTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGAc                                                                                                                                                                                                                                           >  1:438059/1‑248 (MQ=255)                      tAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTAcc                                                                                                                                                                                                                           <  1:243955/250‑1 (MQ=255)                                   gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCCGCGCCAACAAGGTCAGTCGCTCTTCCTGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTCCCATCCCTTTCAttgg                                                                                                                                                                                                             >  1:288126/1‑248 (MQ=255)                                        cgccgcCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCattggattgg                                                                                                                                                                                                        <  2:82711/251‑1 (MQ=255)                                           cgccTTCTGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTAGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGttt                                                                                                                                                                                                     >  1:261527/1‑251 (MQ=255)                                                  tGCGTCGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCCTTGGCTTGGTTTTCAGCtt                                                                                                                                                                                              >  2:360591/1‑251 (MQ=255)                                                       cGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGt                                                                                                                                                                                         >  2:86489/1‑251 (MQ=255)                                                       cGCTACAATGGATTCGATTCC‑CCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGt                                                                                                                                                                                         >  2:184819/1‑251 (MQ=255)                                                          tACAATGGATTCGATTCCACC‑TCGGGCCAGCGCCACCAAGGCGAGAAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCACCGTATTCCAGATCGCAAATTCCTTCACCGTTTACAATACCGACAGAAACCGCGGCTCCCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGtttt                                                                                                                                                                                      >  2:67286/1‑251 (MQ=255)                                                                    cgagtccc‑cc‑TCGGTCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCGTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCGCCGTTTACAATTCCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTTCACCAGCtt                                                                                                                                                                            <  2:227980/244‑1 (MQ=255)                                                                       ttccc‑cc‑TCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGt                                                                                                                                                                        >  2:396126/4‑251 (MQ=255)                                                                              c‑TCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAgcgc                                                                                                                                                                   >  1:396543/1‑250 (MQ=255)                                                                                   ggCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCtg                                                                                                                                                              <  1:122627/251‑2 (MQ=255)                                                                                      aagcgcaaacaaGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAACTTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCCGCTTCTGTAGCGCATCtgca                                                                                                                                                            >  2:69555/8‑247 (MQ=255)                                                                                       agcgCCAACAAGGTGAGTAGCTCATCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCGGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGCCGGTCTCTGCGGCGGAGTCGTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACAGACAGAAACCGCGGCTACCATCCCTTTAATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTACTGTAgccgctctgcc                                                                                                                                                            >  2:372273/4‑240 (MQ=255)                                                                                         cgCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCCTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCCGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGAATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAgcc                                                                                                                                                        >  1:83033/2‑250 (MQ=255)                                                                                            caacaaGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGcc                                                                                                                                                      >  1:254615/1‑250 (MQ=255)                                                                                              acaaGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCCTTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCAc                                                                                                                                                    >  2:356388/1‑250 (MQ=255)                                                                                                    tGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTAcc                                                                                                                                                                                                                           >  2:351291/1‑173 (MQ=255)                                                                                                    tGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTAcc                                                                                                                                                                                                                           <  1:351291/173‑1 (MQ=255)                                                                                                     gAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCAc                                                                                                                                            <  1:262726/251‑1 (MQ=255)                                                                                                       gTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCAccc                                                                                                                                          <  2:306623/251‑1 (MQ=255)                                                                                                       gTAGCCCTTCATGGGTGAACCGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGCTGCGCACGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCAccc                                                                                                                                          >  2:222902/1‑251 (MQ=255)                                                                                                         aGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGt                                                                                                                                        <  1:211593/251‑1 (MQ=255)                                                                                                            tctTCATGGGTGAACGGCTCGCCTTCTGCCGTACCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCTCCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAAt                                                                                                                                     >  1:136764/1‑251 (MQ=255)                                                                                                             ctTCATGGGTGACCGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTaa                                                                                                                                      <  2:358316/249‑1 (MQ=255)                                                                                                              ttCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCGACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCg                                                                                                                                   >  1:164877/1‑251 (MQ=255)                                                                                                              ttCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAAt                                                                                                                                     <  1:50134/249‑1 (MQ=255)                                                                                                              ttCATGGGTGAACGGCTCGCCTTCTGCAGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCCCACAGGCACCCggcat                                                                                                                                     >  2:50134/1‑245 (MQ=255)                                                                                                                 aTGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGaag                                                                                                                                <  1:213632/251‑3 (MQ=255)                                                                                                                    ggTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCgg                                                                                                                             >  2:354385/1‑251 (MQ=255)                                                                                                                                 ccTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACCATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGaagc                                                                                                                               >  1:41527/1‑233 (MQ=255)                                                                                                                                 ccTTCTGCCGTCCCCGTCACTGCAATGAGGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCCCCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCGGTAGCGCATCTGCCATCGCCACACAGGCACCCGTatgcgaagc                                                                                                                               <  2:41527/236‑9 (MQ=255)                                                                                                                                                    cTTCAATGATGCGCCCGTCTTCGGTCATCCCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCACATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCAAAGCGGTACGCGTGCCACCATCAGCCTGACGCACGTCg                                                                                             >  2:207745/1‑251 (MQ=255)                                                                                                                                                           gatgCGCCCGTCTTCGGTCATCCCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTGTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCAGCAGCCTGAAGCACGTCGCAGTCCa                                                                                      <  2:438059/251‑1 (MQ=255)                                                                                                                                                             tgCGCCCTTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCTTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAg                                                                                     <  1:29982/250‑1 (MQ=255)                                                                                                                                                                gcCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGt                                                                                  >  2:90046/1‑250 (MQ=255)                                                                                                                                                                gcCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTa                                                                                 <  1:180941/251‑1 (MQ=255)                                                                                                                                                                      cTTCGGTCATCCCTAAGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTAACATCCCTTTCATTGGATTGGTTTTCCGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCCCCATCAGCCCGACGCACGTCCCCGTCCAGCGTAATGGTg                                                                           >  1:359794/1‑251 (MQ=255)                                                                                                                                                                         cGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAAc                                                                        <  1:32142/251‑1 (MQ=255)                                                                                                                                                                               tcaCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGCTCGCAAATTGCTTCACCGTTTACAATAACGACAGAAACCGCGGCTACCATCCCTTTCCTTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCAccc                                                                  >  1:431867/1‑251 (MQ=255)                                                                                                                                                                                 aCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCAccc                                                                  >  1:98349/1‑249 (MQ=255)                                                                                                                                                                                      gTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCTCGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCtt                                                           <  2:39345/251‑1 (MQ=255)                                                                                                                                                                                              cGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCTACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTTCCACCATCAGCCTGAAGCACGTCGCAGGCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATc                                                    <  2:261527/250‑1 (MQ=255)                                                                                                                                                                                                      gCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACGCACCCAGCGCTTTCAAATCTACTGCcg                                            <  2:43264/250‑1 (MQ=255)                                                                                                                                                                                                           tgagTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGCAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTCATGGTGAACTCACCCAGCGCTTTCAAATCTCCTGCCGCGCGa                                       >  2:75994/2‑250 (MQ=255)                                                                                                                                                                                                           tgagTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGcca                                                                                                                  <  2:41135/174‑1 (MQ=255)                                                                                                                                                                                                           tgagTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGcca                                                                                                                  >  1:41135/2‑175 (MQ=255)                                                                                                                                                                                                           tgagTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAACGCTTTCAAATCTACTGCCGCGCGa                                       >  1:261153/2‑250 (MQ=255)                                                                                                                                                                                                              gTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAagtg                                   >  2:85361/1‑249 (MQ=255)                                                                                                                                                                                                                     aCGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGCAGTGCACGGGc                            >  2:200923/1‑251 (MQ=255)                                                                                                                                                                                                                        tATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGAt                         >  1:243691/1‑251 (MQ=255)                                                                                                                                                                                                                              aGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAACCCGCGGCTACCATCCCTTTCATTGGATTGGTTGTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGatg                   <  2:302192/251‑3 (MQ=255)                                                                                                                                                                                                                                aTCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCt                 <  1:396126/251‑1 (MQ=255)                                                                                                                                                                                                                                          gCTTCACCGTTTACAATACAGACAGAAACCCCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCAGTTTTCCAACAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGCAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCAt       >  1:159969/1‑251 (MQ=255)                                                                                                                                                                                                                                           cTTCACCGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCACATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCAtt      >  1:151756/1‑251 (MQ=255)                                                                                                                                                                                                                                              caccGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATtgtg   <  1:184819/249‑1 (MQ=255)                                                                                                                                                                                                                                               accGTTTACAATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTATAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGAGGTACGCGTGCCACCATCGGCCTGACGCACGTCGCAGTCCAGAGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCCAAGTGCACGGTCGAACAGCCGCTGGTTTTCCAttacgc  >  1:430867/2‑247 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     AAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGC  >  NC_000913/3815807‑3816293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑