Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,813,342	G→C	P204P (CCG→CCC)	dfp →	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,813,342	0	G	C	100.0%	102.4 / NA	33	P204P (CCG→CCC)	dfp	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/19);  total (14/19)

AGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGT  >  NC_000913/3813107‑3813584                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   cgcaggggttcggggccgctgccgctCAGCATAATTTAGGGGTGCTTGCTCCCCGTGGTTTGCTCCAT‑GGGGGG‑CCAGACAGGGGCGGCTAGGCTTGTGGGGATAGGCGCCCTGGGG‑AAATGCGCGATCCGTTAACCATGGTGGAGATGGCAGTAGCTCATTTT‑TCGCCCGCCAACGACCTGAAATAGCGGAACATTATGATGACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATctct                                                                                                                                                                                                                                     <  2:213114/237‑1 (MQ=255)    agATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCCGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAAt                                                                                                                                                                                                                                  <  1:108592/251‑1 (MQ=255)                   cgcccacgactcaTATTTTAGAGTTGCTTGGTTCCGGTGGGTTG‑TC‑CACTGGGGGGCCAGACAGTGGCAGTAAGGCTGGTGGAGATATCGGCCCTGGGC‑GAATGCTCGACCCGTTACCCATGGGGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACTTGAAACATCTGAACATTATTATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAg                                                                                                                                                                                                                   <  2:235279/240‑1 (MQ=255)                       cACGAAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGTTTGTGGAGATATCGGCCCTGGGT‑GAATGCTCGTTCCGTTAACCATTGTGTATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTTAAACATCTGAACATTATGATTTCC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATgg                                                                                                                                                                                                               <  1:1166/251‑1 (MQ=255)                                         ggggctggcttCCCGTGGTTTGCTC‑ATCGGGGGG‑CCAGAAGGTGGCAGTCAGGTTTGTGGAGATATCGGCCCTGGGC‑GATGGCGCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATcgccgccg                                                                                                                                                                                             <  2:128894/244‑1 (MQ=255)                                                        gtgTTTGCTC‑TCCTGGGCG‑CCCGACAGTGCCATTCAGACTTGTGGAGATAGCGG‑CCCGGGCACAATGCTCGTTCCGTTAACCAGTGTTGATATGGCAGTAGC‑CATTTTCTCGCCCGCCACCGACTTGAAACCTCTAAACATTATTATTCCC‑GCCGTCCCGACGCGCGAACCGCTCTATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAGGGGTTTTGCTAGCGCCGCCGTCGCTGCCCTTCGTg                                                                                                                                                                              <  2:217601/249‑1 (MQ=255)                                                         ggTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTg                                                                                                                                                                              <  1:353629/250‑1 (MQ=255)                                                              gCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCATTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGcgcg                                                                                                                                                                         >  1:319820/1‑250 (MQ=255)                                                                   aTCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGt                                                                                                                                                                    >  2:204396/1‑251 (MQ=255)                                                                      gggggg‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATACCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAc                                                                                                                                                                 <  1:204396/250‑1 (MQ=255)                                                                          gg‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTg                                                                                                                                                             >  1:93381/1‑251 (MQ=255)                                                                                  cgggggagttCAGGCTTGTGGAGATACCGGCCCTGGGC‑TAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTATCTCATTTT‑TCGCCCGTCAACGCCCTGAACCACCTGATCATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCGCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGTTATCAg                                                                                                                                                      <  2:130611/242‑1 (MQ=255)                                                                                    gTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGt                                                                                                                                                    >  1:251524/1‑251 (MQ=255)                                                                                    gTGGCAGCCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACCGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATccggg                                                                                                                                                    >  2:24390/1‑247 (MQ=255)                                                                                                             cggctgtGGGC‑GAATGCCCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGCCCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCa                                                                                                                                           <  1:4532/229‑1 (MQ=255)                                                                                                             cggcccTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCa                                                                                                                                           >  2:4532/5‑235 (MQ=255)                                                                                                               cccgggggC‑GAATGCGCGACCCGTAACCCATGGGGGATATGGCAGTAGTCCATTTT‑TCGCCCGTCAACGCCCTAAAACATCAGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCGCTAATCACAGCTCCGGCAAGAGGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGttt                                                                                                                         <  2:348147/246‑1 (MQ=255)                                                                                                                   tGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGtt                                                                                                                      <  1:15901/250‑1 (MQ=255)                                                                                                                                         aaCCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGCTTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACcg                                                                                                >  2:396458/1‑251 (MQ=255)                                                                                                                                               tgtgGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTg                                                                                           <  1:12129/250‑1 (MQ=255)                                                                                                                                                               gttaagttt‑tCCCCCGTCAACGCCCTAAAACATTTGAACTTTA‑GATTACCCGCCGGCCCGACGCGTGACCCGCTCGAGCCCGTGCGTTATATCTCAAATCCCATCTCCGGCAAGATGGGTGTTGCTACCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTAACAGGCCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTGGATGGTATGACCGCGCTGGAAATGGAATGCGCCGt                                                                          <  2:192606/245‑1 (MQ=255)                                                                                                                                                                       t‑tCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCtt                                                                  >  2:147732/1‑251 (MQ=255)                                                                                                                                                                             ccGTAAACGACCTGAAACATCTGAACATTATGATGACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTa                                                             <  1:298545/251‑1 (MQ=255)                                                                                                                                                                                   acgtcctaaaccATCTGAACATTATGTTTCCC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCTACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCGGGAAATGGAAGCCGCCGTGAATGCTTCTGTAcagcag                                                       <  2:64507/240‑1 (MQ=255)                                                                                                                                                                                               aTCTGAACATTATGATTACC‑GCCGGCCAGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATAGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCCGCAAAATAttttt                                           >  2:396098/1‑251 (MQ=255)                                                                                                                                                                                                   gAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCg                                       >  1:301639/1‑251 (MQ=255)                                                                                                                                                                                                    tcgtatctGATTCCC‑GCCGGCCCGACGCGTGAACCGCTCGTTCCCGTGCTTTATATCTCTAACCACAGCTCCGGCAAGATGGGTTTTGCTTTCGCCGCCGCCGCTGCCCGGCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATc                                        <  2:86337/242‑1 (MQ=255)                                                                                                                                                                                                       aTTAGGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTg                                   <  1:153111/251‑1 (MQ=255)                                                                                                                                                                                                            gATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATc                                        >  1:86337/1‑241 (MQ=255)                                                                                                                                                                                                                     ccgGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCg                      >  1:5514/1‑251 (MQ=255)                                                                                                                                                                                                                       ggCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGCACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCgg                     >  2:171330/1‑250 (MQ=255)                                                                                                                                                                                                                        gCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGAt                   >  1:123928/1‑251 (MQ=255)                                                                                                                                                                                                                                           cGATCCCGTGCGTTATACCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGt  <  1:5619/249‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   AGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACC‑GCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGT  >  NC_000913/3813107‑3813584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑