Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,813,258	G→A	A176A (GCG→GCA)	dfp →	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,813,258	0	G	A	100.0%	112.4 / NA	38	A176A (GCG→GCA)	dfp	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/23);  total (15/23)

TGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTG  >  NC_000913/3813015‑3813495                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               tgccgttgtTGCTGCCGTAATGGCGAATGCCCTGGTATCGACGTTTTGTCTGGCTACCCCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAgct                                                                                                                                                                                                                                          <  1:331353/244‑2 (MQ=255)   cccGTGATGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCAt                                                                                                                                                                                                                                       >  1:110291/1‑251 (MQ=255)               ccGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGGGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTc                                                                                                                                                                                                                          <  1:303154/251‑1 (MQ=255)                       gggAATGCCCTGGTACCGAGGTTTTGTCTGGCTACCCCTGCGCC‑TGTAGCCGTCCCCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCt                                                                                                                                                                                                                  <  2:217083/249‑1 (MQ=255)                         gAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACCACCTg                                                                                                                                                                                                                 >  2:237493/1‑250 (MQ=255)                                   gTATCGACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGCTCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGa                                                                                                                                                                                                       >  1:219494/1‑250 (MQ=255)                                    tATCGACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAAc                                                                                                                                                                                                     >  1:64507/1‑251 (MQ=255)                                      tCGACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACAt                                                                                                                                                                                                   >  1:128894/1‑251 (MQ=255)                                       cgacgaTGTTTCCGGCTACCCCTG‑GCCGTGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATAGTGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACAtt                                                                                                                                                                                                  <  2:52774/251‑1 (MQ=255)                                         acgaTTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAGTTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGAAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTAt                                                                                                                                                                                                >  1:66846/1‑251 (MQ=255)                                                 gctgctgtaacccgcgcC‑TGCGGCCGTTCCCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACTCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑GTCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCTTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTAcc                                                                                                                                                                                         <  2:171815/237‑1 (MQ=255)                                                    ggCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTAccgccg                                                                                                                                                                                     >  1:348147/1‑251 (MQ=255)                                                        cacccctgcccctgaagcgttgcccccc‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGcc                                                                                                                                                                                  <  1:22703/227‑1 (MQ=255)                                                        acacCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATACTTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGGCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGccc                                                                                                                                                                                 >  2:5619/1‑251 (MQ=255)                                                          ccccggcgccggggcccggccccccc‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTGAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCGGGGC‑GAATGCGCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCg                                                                                                                                                                                <  2:166799/229‑1 (MQ=255)                                                             ccgcactgtatgccgtccccccc‑GCCATGAACCAGCAGATGTCCCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGAct                                                                                                                                                                             <  2:12918/232‑2 (MQ=255)                                                                           gTGCTCCCCCGCCATGAACCAGCAGATGTCCGGTGCCGCGGCCACGCAGCATATTTTAGAGGTGCTTGCTTCCCGGGGTTTGATC‑ATCTGGGGG‑CCAGACGGTGGCAGGCAGGCGTGGGGAGATAGCGTCCCTGGGT‑GAATGCGCGACCCGTTACCCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGGCCGGAAACATTTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCg                                                                                                                                                                <  2:66846/251‑1 (MQ=255)                                                                                cccc‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGGGGTTTGCTC‑ATCGGGGGG‑CCAGACAGGGGCAGTCGGGCTTGTGGAGATAGCGGCCCTGGGG‑GAATGCTCGATCCGTTAACGATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACTTCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATcc                                                                                                                                                            <  1:40314/249‑1 (MQ=255)                                                                                               cgcaggggttcggggccgctgccgctCAGCATAATTTAGGGGTGCTTGCTCCCCGTGGTTTGCTCCAT‑GGGGGG‑CCAGACAGGGGCGGCTAGGCTTGTGGGGATAGGCGCCCTGGGG‑AAATGCGCGATCCGTTAACCATGGTGGAGATGGCAGTAGCTCATTTT‑TCGCCCGCCAACGACCTGAAATAGCGGAACATTATGATGACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATctct                                                                                                                                            <  2:213114/237‑1 (MQ=255)                                                                                                  agATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCCGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAAt                                                                                                                                         <  1:108592/251‑1 (MQ=255)                                                                                                                 cgcccacgactcaTATTTTAGAGTTGCTTGGTTCCGGTGGGTTG‑TC‑CACTGGGGGGCCAGACAGTGGCAGTAAGGCTGGTGGAGATATCGGCCCTGGGC‑GAATGCTCGACCCGTTACCCATGGGGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACTTGAAACATCTGAACATTATTATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAg                                                                                                                          <  2:235279/240‑1 (MQ=255)                                                                                                                     cACGAAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGTTTGTGGAGATATCGGCCCTGGGT‑GAATGCTCGTTCCGTTAACCATTGTGTATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTTAAACATCTGAACATTATGATTTCCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATgg                                                                                                                      <  1:1166/251‑1 (MQ=255)                                                                                                                                       ggggctggcttCCCGTGGTTTGCTC‑ATCGGGGGG‑CCAGAAGGTGGCAGTCAGGTTTGTGGAGATATCGGCCCTGGGC‑GATGGCGCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATcgccgccg                                                                                                    <  2:128894/244‑1 (MQ=255)                                                                                                                                                      gtgTTTGCTC‑TCCTGGGCG‑CCCGACAGTGCCATTCAGACTTGTGGAGATAGCGG‑CCCGGGCACAATGCTCGTTCCGTTAACCAGTGTTGATATGGCAGTAGC‑CATTTTCTCGCCCGCCACCGACTTGAAACCTCTAAACATTATTATTCCCGCCGTCCCGACGCGCGAACCGCTCTATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAGGGGTTTTGCTAGCGCCGCCGTCGCTGCCCTTCGTg                                                                                     <  2:217601/249‑1 (MQ=255)                                                                                                                                                       ggTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTg                                                                                     <  1:353629/250‑1 (MQ=255)                                                                                                                                                            gCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCATTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGcgcg                                                                                >  1:319820/1‑250 (MQ=255)                                                                                                                                                                 aTCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGt                                                                           >  2:204396/1‑251 (MQ=255)                                                                                                                                                                    gggggg‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATACCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAc                                                                        <  1:204396/250‑1 (MQ=255)                                                                                                                                                                        gg‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTg                                                                    >  1:93381/1‑251 (MQ=255)                                                                                                                                                                                cgggggagttCAGGCTTGTGGAGATACCGGCCCTGGGC‑TAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTATCTCATTTT‑TCGCCCGTCAACGCCCTGAACCACCTGATCATTATGATTACCGCCGGCCCGACGCGTGAACCGCGCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGTTATCAg                                                             <  2:130611/242‑1 (MQ=255)                                                                                                                                                                                  gTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGt                                                           >  1:251524/1‑251 (MQ=255)                                                                                                                                                                                  gTGGCAGCCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACCGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATccggg                                                           >  2:24390/1‑247 (MQ=255)                                                                                                                                                                                                           cggctgtGGGC‑GAATGCCCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGCCCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCa                                                  <  1:4532/229‑1 (MQ=255)                                                                                                                                                                                                           cggcccTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCa                                                  >  2:4532/5‑235 (MQ=255)                                                                                                                                                                                                             cccgggggC‑GAATGCGCGACCCGTAACCCATGGGGGATATGGCAGTAGTCCATTTT‑TCGCCCGTCAACGCCCTAAAACATCAGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCGCTAATCACAGCTCCGGCAAGAGGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGttt                                <  2:348147/246‑1 (MQ=255)                                                                                                                                                                                                                 tGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGtt                             <  1:15901/250‑1 (MQ=255)                                                                                                                                                                                                                                       aaCCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGCTTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACcg       >  2:396458/1‑251 (MQ=255)                                                                                                                                                                                                                                             tgtgGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTg  <  1:12129/250‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               TGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTG  >  NC_000913/3813015‑3813495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑