Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,813,207	T→A	G159G (GGT→GGA)	dfp →	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,813,207	0	T	A	97.8%	137.6 / NA	45	G159G (GGT→GGA)	dfp	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (17/27);  minor base G (0/1);  total (17/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTGATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGT  >  NC_000913/3812973‑3813438                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          ggCTTATTTAGTGATTCTCGCCCTTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCCCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCTTGGGc                                                                                                                                                                                                                           <  1:356974/251‑1 (MQ=255)              aTTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGACTGACCTGGTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATAGGCCCTGGGC‑GAATGcagcgcgg                                                                                                                                                                                                             >  2:22335/1‑244 (MQ=255)              aTTCCCGCCCCGGCCACGGCAGATTTGATTCCCGGTGTTGCTGCCGGAATGGCGAATGACCTGGAACC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGc                                                                                                                                                                                                                    <  1:22335/244‑1 (MQ=255)                tctcGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGGCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGt                                                                                                                                                                                                           >  2:12129/1‑251 (MQ=255)                 cgcGCCCCGGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAAGGGGGAATGACCTGGTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGtt                                                                                                                                                                                                          <  1:393269/249‑1 (MQ=255)                          gCCACGGCAGATTTGTGTGCCCGTGTGGCTCCCGGAATGGCGAATGACCTGTTATC‑GACGTTTTGTCTGGCAACACCTGCGCC‑GGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGGTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATtg                                                                                                                                                                                                  <  1:208006/250‑1 (MQ=255)                               ggCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCCTAATTTAGAGGTGCTTGCTTCACGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGTAGGCAGGCTTGTGGAGATATAGGCCCTGGGC‑GAATGCTCGATACGTTAACCATAGTggct                                                                                                                                                                                             >  2:299171/1‑248 (MQ=255)                                gtaGATTGGATTGCCGGTGTTCCTGCCGTAAGGGCGAATGACGTGGTATC‑GACTGTTTGTCGGGCTACCCCTGCGCC‑TGTAGCCGGGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTGTGCGC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCGTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTGAACCATTGTGGat                                                                                                                                                                                             <  1:285201/247‑1 (MQ=255)                                  agattggtttGCCCGTGTGGCTGCCGGAATGGCGAATGCCCTGGGACC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTGGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGAGATgg                                                                                                                                                                                         <  2:292053/243‑1 (MQ=255)                                  aaaTTTGATTGCCCGTGTTGCTGCCGGAAGGGCGAATGACCGGGAAGC‑GACGATTGGGCTGGATACACTGGCGCC‑TGTAGCCGTGCACCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑AGCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGTCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATgg                                                                                                                                                                                         <  1:184489/249‑1 (MQ=255)                                    aTTTGATTGCCTGTGTTGCTGCCGAAGGGGCGAATG‑CCCGGTTTCCGCCGATTTGTCTGGCTCCCCCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGca                                                                                                                                                                                       <  2:17720/251‑2 (MQ=255)                                       tGATGGCCCGTGTTGCTGCCGGAATGGCGAATGACTTGGTATC‑GACGATTTGTCTGGCTACACCGCCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGTGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGcagt                                                                                                                                                                                     <  1:287395/250‑4 (MQ=255)                                        gATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGattgggcagg                                                                                                                                                                                     >  2:299928/1‑241 (MQ=255)                                           tgccgttgtTGCTGCCGTAATGGCGAATGCCCTGGTATC‑GACGTTTTGTCTGGCTACCCCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAgct                                                                                                                                                                                 <  1:331353/244‑2 (MQ=255)                                             cccGTGATGCTGCCGGAATGGCGAATGACCTGGTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCAt                                                                                                                                                                              >  1:110291/1‑251 (MQ=255)                                                         ccGGAATGGCGAATGACCTGGTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGGGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTc                                                                                                                                                                 <  1:303154/251‑1 (MQ=255)                                                                 gggAATGCCCTGGTACC‑GAGGTTTTGTCTGGCTACCCCTGCGCC‑TGTAGCCGTCCCCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCt                                                                                                                                                         <  2:217083/249‑1 (MQ=255)                                                                   gAATGACCTGGTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACCACCTg                                                                                                                                                        >  2:237493/1‑250 (MQ=255)                                                                             gTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGCTCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGa                                                                                                                                              >  1:219494/1‑250 (MQ=255)                                                                              tATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAAc                                                                                                                                            >  1:64507/1‑251 (MQ=255)                                                                                tC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACAt                                                                                                                                          >  1:128894/1‑251 (MQ=255)                                                                                 c‑gacgaTGTTTCCGGCTACCCCTG‑GCCGTGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATAGTGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACAtt                                                                                                                                         <  2:52774/251‑1 (MQ=255)                                                                                    acgaTTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAGTTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGAAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTAt                                                                                                                                       >  1:66846/1‑251 (MQ=255)                                                                                            gctgctgtaacccgcgcC‑TGCGGCCGTTCCCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACTCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑GTCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCTTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTAcc                                                                                                                                <  2:171815/237‑1 (MQ=255)                                                                                               ggCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTAccgccg                                                                                                                            >  1:348147/1‑251 (MQ=255)                                                                                                   cacccctgcccctgaagcgttgcccccc‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGcc                                                                                                                         <  1:22703/227‑1 (MQ=255)                                                                                                   acacCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATACTTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGGCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGccc                                                                                                                        >  2:5619/1‑251 (MQ=255)                                                                                                     ccccggcgccggggcccggccccccc‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTGAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCGGGGC‑GAATGCGCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCg                                                                                                                       <  2:166799/229‑1 (MQ=255)                                                                                                        ccgcactgtatgccgtccccccc‑GCCATGAACCAGCAGATGTCCCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGAct                                                                                                                    <  2:12918/232‑2 (MQ=255)                                                                                                                      gTGCTCCCCCGCCATGAACCAGCAGATGTCCGGTGCCGCGGCCACGCAGCATATTTTAGAGGTGCTTGCTTCCCGGGGTTTGATC‑ATCTGGGGG‑CCAGACGGTGGCAGGCAGGCGTGGGGAGATAGCGTCCCTGGGT‑GAATGCGCGACCCGTTACCCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGGCCGGAAACATTTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCg                                                                                                       <  2:66846/251‑1 (MQ=255)                                                                                                                           cccc‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGGGGTTTGCTC‑ATCGGGGGG‑CCAGACAGGGGCAGTCGGGCTTGTGGAGATAGCGGCCCTGGGG‑GAATGCTCGATCCGTTAACGATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACTTCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATcc                                                                                                   <  1:40314/249‑1 (MQ=255)                                                                                                                                          cgcaggggttcggggccgctgccgctCAGCATAATTTAGGGGTGCTTGCTCCCCGTGGTTTGCTCCAT‑GGGGGG‑CCAGACAGGGGCGGCTAGGCTTGTGGGGATAGGCGCCCTGGGG‑AAATGCGCGATCCGTTAACCATGGTGGAGATGGCAGTAGCTCATTTT‑TCGCCCGCCAACGACCTGAAATAGCGGAACATTATGATGACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATctct                                                                                   <  2:213114/237‑1 (MQ=255)                                                                                                                                             agATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCCGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAAt                                                                                <  1:108592/251‑1 (MQ=255)                                                                                                                                                            cgcccacgactcaTATTTTAGAGTTGCTTGGTTCCGGTGGGTTG‑TC‑CACTGGGGGGCCAGACAGTGGCAGTAAGGCTGGTGGAGATATCGGCCCTGGGC‑GAATGCTCGACCCGTTACCCATGGGGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACTTGAAACATCTGAACATTATTATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAg                                                                 <  2:235279/240‑1 (MQ=255)                                                                                                                                                                cACGAAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGTTTGTGGAGATATCGGCCCTGGGT‑GAATGCTCGTTCCGTTAACCATTGTGTATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTTAAACATCTGAACATTATGATTTCCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATgg                                                             <  1:1166/251‑1 (MQ=255)                                                                                                                                                                                  ggggctggcttCCCGTGGTTTGCTC‑ATCGGGGGG‑CCAGAAGGTGGCAGTCAGGTTTGTGGAGATATCGGCCCTGGGC‑GATGGCGCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATcgccgccg                                           <  2:128894/244‑1 (MQ=255)                                                                                                                                                                                                 gtgTTTGCTC‑TCCTGGGCG‑CCCGACAGTGCCATTCAGACTTGTGGAGATAGCGG‑CCCGGGCACAATGCTCGTTCCGTTAACCAGTGTTGATATGGCAGTAGC‑CATTTTCTCGCCCGCCACCGACTTGAAACCTCTAAACATTATTATTCCCGCCGTCCCGACGCGCGAACCGCTCTATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAGGGGTTTTGCTAGCGCCGCCGTCGCTGCCCTTCGTg                            <  2:217601/249‑1 (MQ=255)                                                                                                                                                                                                  ggTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTg                            <  1:353629/250‑1 (MQ=255)                                                                                                                                                                                                       gCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCATTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGcgcg                       >  1:319820/1‑250 (MQ=255)                                                                                                                                                                                                            aTCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGt                  >  2:204396/1‑251 (MQ=255)                                                                                                                                                                                                               gggggg‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATACCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAc               <  1:204396/250‑1 (MQ=255)                                                                                                                                                                                                                   gg‑CCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTg           >  1:93381/1‑251 (MQ=255)                                                                                                                                                                                                                           cgggggagttCAGGCTTGTGGAGATACCGGCCCTGGGC‑TAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTATCTCATTTT‑TCGCCCGTCAACGCCCTGAACCACCTGATCATTATGATTACCGCCGGCCCGACGCGTGAACCGCGCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGTTATCAg    <  2:130611/242‑1 (MQ=255)                                                                                                                                                                                                                             gTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGt  >  1:251524/1‑251 (MQ=255)                                                                                                                                                                                                                             gTGGCAGCCAGGCTTGTGGAGATATCGGCCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACCGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATccggg  >  2:24390/1‑247 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          GGCTGATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATC‑GACGATTTGTCTGGCTACACCTGCGCC‑TGTAGCCGTGCTCCCC‑GCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTC‑ATCTGGGGG‑CCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGC‑GAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTT‑TCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGT  >  NC_000913/3812973‑3813438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑