Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,812,729	T→A	intergenic (‑170/‑2)	yicR ← / → dfp	RadC‑like JAB domain‑containing protein YicR/fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,812,729	0	T	A	100.0%	96.9 / NA	30	intergenic (‑170/‑2)	yicR/dfp	RadC‑like JAB domain‑containing protein YicR/fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (10/20);  total (10/20)

ATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGAATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTTCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGCGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTGCAGGCGG‑TTTCTGGTTATCCCGTTTCCGACAGTCTGCTGGACCCGGCAGCCGAAGCCGCTATGGGCCATATTGAGCT  >  NC_000913/3812488‑3812963                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             aTCCGGTAAGTCGCTAATACCAAACTTCCGCATTTTTTCGCGCGGCATCAACAGCTGTGCAGTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCt                                                                                                                                                                                                                                     <  2:103838/251‑1 (MQ=255)        aaGGCGCTAATACCAACCTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGt                                                                                                                                                                                                                              <  2:13520/251‑1 (MQ=255)        aaGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATAAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCACTCCTGGAAAGTGCCTCGCATAGTGAATCTCTGGTTAAGGCACGACAACAATCGTCTTGTGATAAAATCGCCAACTTCTGGTTTCACCCTACAGGAAACATCAACATGAGACTGGACGGt                                                                                                                                                                                                                              >  2:184489/1‑251 (MQ=255)                           tCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCt                                                                                                                                                                                                                                     <  2:14693/225‑1 (MQ=255)                           tCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCt                                                                                                                                                                                                                                     >  1:14693/1‑225 (MQ=255)                           tCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGc                                                                                                                                                                                                            <  1:162401/250‑1 (MQ=255)                            cAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCg                                                                                                                                                                                                           <  1:95765/250‑1 (MQ=255)                                    tttCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCCTAGCAGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGACGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAACAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAgcggcg                                                                                                                                                                                                  >  1:411062/1‑251 (MQ=255)                                    tttCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGGCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATATCAGGTGAAGGCACGACAACAAAAGTATTGTGCTCAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAgcggcg                                                                                                                                                                                                  >  2:15901/1‑251 (MQ=255)                                        gcgcgGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTAt                                                                                                                                                                                              <  1:238422/251‑1 (MQ=255)                                                  aaCAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGTTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCGCGCAAAGTGAATCTCAGGTGAAGGCACAACAACAAAAGTATTGTGATAAAATCGCCAACGTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAAACGTTCACGGCGTTAGCGGCGGTATT‑GCTGCCtat                                                                                                                                                                                   <  2:42672/251‑1 (MQ=255)                                                               tGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGaa                                                                                                                                                                       <  1:137118/250‑1 (MQ=255)                                                                        ccttcacctCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTTCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTTATAAAATCGCCAACTTCTGGTGTCACCCTGCAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGcgtc                                                                                                                                                             <  1:192066/251‑1 (MQ=255)                                                                         cttcacctCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑TCTGCCTCTAAAAACCCCGCAATGGTGcgtcg                                                                                                                                                            >  1:182724/1‑251 (MQ=255)                                                                             acctCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGATTT‑GCTGCCCTTAAAAACCCTTAACCTGTGCGGCGtttt                                                                                                                                                        >  1:12918/1‑250 (MQ=255)                                                                               ctcctTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑TCTGCCTATAAAAACCCTGAACTGGTGCGTCGttttcg                                                                                                                                                      >  1:58256/1‑248 (MQ=255)                                                                                 cctTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAAACGACGGCCAGTTTTCAATCCTGGAAAGTGCTTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTATGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAACCCCCTGAACTTGGGCGTCGTGTgcgct                                                                                                                                                    <  2:240505/251‑2 (MQ=255)                                                                                                  tCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGCATTTGTGGCCCTTAAAACCC‑TGAACTGGGGCGGCGGTTGTGCGGTCGCGGGGCCgcggcc                                                                                                                                   >  1:52774/1‑246 (MQ=255)                                                                                                                 tccGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACGTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGCTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGAGCGCGGGGCCGACGTCCGCGTAGCCATGAc                                                                                                                     <  2:237759/248‑1 (MQ=255)                                                                                                                  ccGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGAcc                                                                                                                    <  1:404127/249‑1 (MQ=255)                                                                                                                               ggCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCCCAGGTGAAGTCACGACAACAAAAGTATTTTGATAAAATCGCCAACTTCTGGTGTCACCCCACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAACCCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGc                                                                                                      <  1:97381/251‑1 (MQ=255)                                                                                                                                                         cTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCCGAAATGGTGGGTGGTTTGGGCGGTCGCGGGGCCGACGCCCGCGCAGCCATGACCGAAGCGGGACAAGcccttttatccccacctcgattgccgg                                                                            >  2:278549/1‑226 (MQ=255)                                                                                                                                                                    aaTCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTGCAGGCGG‑TTTCTGGt                                                                <  1:50056/251‑1 (MQ=255)                                                                                                                                                                                           caaAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGGATT‑GCTTCCTATAAAAACCCCGAACAGGTGCGGCGTTTATGCGGGCCCGGGGGCGACGTCCCCGTAACCCAGACCGAAACGGCCAAAACCTTTATTACCCCCCCTCGCTTGCAAGCGGGTTTCTGGtttaccaggtacgacccatttct                                          >  2:246663/1‑230 (MQ=255)                                                                                                                                                                                                  aTTGTGTTAAAATCGACAACTTCTTGTGTCACCCTACAGGAAAAATCAACATGATCCTGGCCGGTAAAAAAATCGTTCGCGGCGGTAGCGGCGGTATT‑GCTGCCTATAAAACGCCTGATCTGGTGCGTCGTTTGCGCGATCGCGGTTCCGTCGTCCGCGTAGCCATGACCGAAGCGGCAAAATCCTTTATCACCCCTCGTATCTTGCAGGCGG‑TTTCTGGTTATCCCGGTTCCGTCAGttggcttgaccc                                   <  2:222239/250‑12 (MQ=255)                                                                                                                                                                                                               cGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTTCCTATAAAACCCCTGa                                                                                                                                                                        <  1:305890/105‑1 (MQ=255)                                                                                                                                                                                                                 ccAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTGCAGGCGG‑TTTCTGGTTATCCCGTTTCCGACAGTTTGCTGGACCCGGCAGCCGAAGCCGCt                   <  1:246663/251‑1 (MQ=255)                                                                                                                                                                                                                     cTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTGCAGGCGG‑TTTCTGGTTATCCCGTTTCCGACAGTttgc                                          <  1:98332/224‑1 (MQ=255)                                                                                                                                                                                                                               cACCCTACAGGAAAAATCAACTTGAGCCTGGCCGGTAAAAAAATCGTTCGCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTGCAGGCGG‑TTTCTGGTTATCCCGTTTCCGACAGTTTGCTGGACCCGGCAGCCGAAGCCGCTATGGGCCATATTg      <  2:53699/250‑1 (MQ=255)                                                                                                                                                                                                                                  ccTACAGGAAAAAGCAACATGGGCCGTGCCGGTAAAAAAATCGTACTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCAGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCGCTTAGCTTGCAGGCGG‑TTTCTGGTTATCCCGTTTCCGACAGTTTGCTGGACCCGGCAGCCGAAGCCGCTATGGGCCATAGTGAGCt  <  2:179604/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             ATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGAATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTTCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGCGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTGCAGGCGG‑TTTCTGGTTATCCCGTTTCCGACAGTCTGCTGGACCCGGCAGCCGAAGCCGCTATGGGCCATATTGAGCT  >  NC_000913/3812488‑3812963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑