Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,812,637 C→T intergenic (‑78/‑94) yicR ← / → dfp RadC‑like JAB domain‑containing protein YicR/fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,812,6370CT97.3% 112.7 / NA 37intergenic (‑78/‑94)yicR/dfpRadC‑like JAB domain‑containing protein YicR/fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (17/19);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGAATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTTCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGCGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGC  >  NC_000913/3812406‑3812864
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    
aaTTCTCCAGCACCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAAtctc                                                                                                                                                                                                                   >  2:404127/1‑251 (MQ=255)
                     ccAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCTGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACaaa                                                                                                                                                                                              <  2:321576/251‑1 (MQ=255)
                                    cTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTACGGCCCTAATACCAAACTTCAGCATTTTTTCGAGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTCAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATaaa                                                                                                                                                                                >  2:382494/1‑250 (MQ=255)
                                      ttACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATaaa                                                                                                                                                                                <  1:111213/248‑1 (MQ=255)
                                      ttACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAAt                                                                                                                                                                              <  1:182435/250‑1 (MQ=255)
                                      ttACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAAGACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCGCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGGTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGGATTGTGAGAAAAt                                                                                                                                                                              <  2:146581/250‑1 (MQ=255)
                                      ttACCGCGCGATCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCACCAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCGTCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCCATCCTGGAAAGTGCCTCGCAAAGTGAATCTCCGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATc                                                                                                                                                                             >  2:178376/1‑251 (MQ=255)
                                        aCCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTCAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTCTTGTGATAAAATCGc                                                                                                                                                                           >  2:176177/1‑251 (MQ=255)
                                           gcgcgTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCa                                                                                                                                                                         >  2:137118/1‑250 (MQ=255)
                                           gcgcgTTACGGTACGAAGAACTAGCTCAAGCAGATCGACATCCGTTATAGCGCTAACTCCAAACTTAAGCATTTTTACGCGCGGAATCAACCGCTCTGCATTGTTTTTCACCTTCACCTCATTTGTGGAGCTCGCATCCTGTCTTAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGACTCTAAGGCGAAGCCACGACACAAAAAGTTTGGTGATAAAATAGCCa                                                                                                                                                                         >  2:427196/1‑250 (MQ=255)
                                               gTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTc                                                                                                                                                                    >  1:13520/1‑251 (MQ=255)
                                                    agcaCGCAGCAACAGCGCCAGCCGCTCGACATCCGGTACGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCACCAACAGCCGCGCATTAGTCTTCCCCCCCACCTCCTTTGTCATGCTCGCATCCTGTCCTCGCCGCTCCGATAAATCCCCGACCAGTTTTCCCTCCTCCACAGCGCCTCGCAACGACAATCCCAGGTGACGGCACCACCACAAACGAATTGCGCTAAAACCGCCCACttatgcgg                                                                                                                                                               >  2:323290/4‑245 (MQ=255)
                                                      tACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTc                                                                                                                                                             >  2:331353/1‑251 (MQ=255)
                                                         gCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCAcc                                                                                                                                                          <  1:337633/251‑1 (MQ=255)
                                                                                  aTCCGGTAAGTCGCTAATACCAAACTTCCGCATTTTTTCGCGCGGCATCAACAGCTGTGCAGTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCt                                                                                                                                 <  2:103838/251‑1 (MQ=255)
                                                                                         aaGGCGCTAATACCAACCTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGt                                                                                                                          <  2:13520/251‑1 (MQ=255)
                                                                                         aaGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATAAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCACTCCTGGAAAGTGCCTCGCATAGTGAATCTCTGGTTAAGGCACGACAACAATCGTCTTGTGATAAAATCGCCAACTTCTGGTTTCACCCTACAGGAAACATCAACATGAGACTGGACGGt                                                                                                                          >  2:184489/1‑251 (MQ=255)
                                                                                                    aCCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGaca                                                                                                                                                                                                   <  2:85964/167‑1 (MQ=255)
                                                                                                    aCCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGaca                                                                                                                                                                                                   >  1:85964/1‑167 (MQ=255)
                                                                                                            tCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCt                                                                                                                                 <  2:14693/225‑1 (MQ=255)
                                                                                                            tCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCt                                                                                                                                 >  1:14693/1‑225 (MQ=255)
                                                                                                            tCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGc                                                                                                        <  1:162401/250‑1 (MQ=255)
                                                                                                             cAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCg                                                                                                       <  1:95765/250‑1 (MQ=255)
                                                                                                                     tttCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCCTAGCAGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGACGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAACAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAgcggcg                                                                                              >  1:411062/1‑251 (MQ=255)
                                                                                                                     tttCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGGCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATATCAGGTGAAGGCACGACAACAAAAGTATTGTGCTCAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAgcggcg                                                                                              >  2:15901/1‑251 (MQ=255)
                                                                                                                         gcgcgGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTAt                                                                                          <  1:238422/251‑1 (MQ=255)
                                                                                                                                   aaCAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGTTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCGCGCAAAGTGAATCTCAGGTGAAGGCACAACAACAAAAGTATTGTGATAAAATCGCCAACGTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAAACGTTCACGGCGTTAGCGGCGGTATT‑GCTGCCtat                                                                               <  2:42672/251‑1 (MQ=255)
                                                                                                                                                tGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGaa                                                                   <  1:137118/250‑1 (MQ=255)
                                                                                                                                                         ccttcacctCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTTCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTTATAAAATCGCCAACTTCTGGTGTCACCCTGCAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGcgtc                                                         <  1:192066/251‑1 (MQ=255)
                                                                                                                                                          cttcacctCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑TCTGCCTCTAAAAACCCCGCAATGGTGcgtcg                                                        >  1:182724/1‑251 (MQ=255)
                                                                                                                                                              acctCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGATTT‑GCTGCCCTTAAAAACCCTTAACCTGTGCGGCGtttt                                                    >  1:12918/1‑250 (MQ=255)
                                                                                                                                                                ctcctTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑TCTGCCTATAAAAACCCTGAACTGGTGCGTCGttttcg                                                  >  1:58256/1‑248 (MQ=255)
                                                                                                                                                                  cctTTGTGGTGCTCGCATCCTGTCATAGCCGTTCCGTTAAAACGACGGCCAGTTTTCAATCCTGGAAAGTGCTTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTATGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAACCCCCTGAACTTGGGCGTCGTGTgcgct                                                <  2:240505/251‑2 (MQ=255)
                                                                                                                                                                                   tCCTGTCATAGCCGTTCCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGCATTTGTGGCCCTTAAAACCC‑TGAACTGGGGCGGCGGTTGTGCGGTCGCGGGGCCgcggcc                               >  1:52774/1‑246 (MQ=255)
                                                                                                                                                                                                  tccGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACGTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGCTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGAGCGCGGGGCCGACGTCCGCGTAGCCATGAc                 <  2:237759/248‑1 (MQ=255)
                                                                                                                                                                                                   ccGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGAcc                <  1:404127/249‑1 (MQ=255)
                                                                                                                                                                                                                ggCCAGTTTTCAATCCTGGAAAGTGCCTCGCAAAGTGAATCCCAGGTGAAGTCACGACAACAAAAGTATTTTGATAAAATCGCCAACTTCTGGTGTCACCCCACAGGAAAAATCAACATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAACCCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGc  <  1:97381/251‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    
AATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGAATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGCATCCTGTCATAGCCGTTTCGTTAAATCGACGGCCAGTTTTCAATCCTGGAAAGCGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATT‑GCTGCCTATAAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGC  >  NC_000913/3812406‑3812864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑