Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,812,338	C→T	V74V (GTG→GTA)	yicR ←	RadC‑like JAB domain‑containing protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,812,338	0	C	T	100.0%	136.0 / NA	41	V74V (GTG→GTA)	yicR	RadC‑like JAB domain‑containing protein YicR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)

CCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCCACGCCAATTCCATGAACGCCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGAATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGC  >  NC_000913/3812100‑3812583                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      cccGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTcc                                                                                                                                                                                                                                            >  1:321576/1‑250 (MQ=255)  ccGAGGATGGACTTCAACATGGGTGAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCGCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTGTCTTCACGCAGCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCAt                                                                                                                                                                                                                                          <  1:181557/251‑1 (MQ=255)                      gggttAGCGTGCCGGCAACAAACCGGCTATGCGTTATACCCCGGAGTTGTGCGTCGAGAAAGCTAAACATAACGATCTACCGCTCCTCACCCGTGAGCTCGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGAATCCGCACGTTGTCGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAAATGTTc                                                                                                                                                                                                                       >  2:268701/4‑250 (MQ=255)                      ggTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTc                                                                                                                                                                                                                       >  1:143753/1‑250 (MQ=255)                                  cGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTc                                                                                                                                                                                                                                             <  1:157397/216‑1 (MQ=255)                                  cGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTc                                                                                                                                                                                                                                             >  2:157397/1‑216 (MQ=255)                                                  aTGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAagag                                                                                                                                                                                          <  1:273905/251‑1 (MQ=255)                                                     cGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCACTCCCCTTTAACTGGGCAACTTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACACGCCATAAAGAGAGc                                                                                                                                                                                       >  1:222239/1‑251 (MQ=255)                                                          taacccggtggtttggtgggagtaaGACCACCATAAAGATCTCCCGCACCGAACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCGTCTGGCTGTGTCAAGGGCTTTCTACGCGCTTCGACGCGTTGTAGGATCCTCTCGCCAGTTCAGCAGACCCCTTAAACGGGTCTAATTTCGCTACGCCAATGCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGaa                                                                                                                                                                                   <  1:176346/227‑1 (MQ=255)                                                               cGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATtctc                                                                                                                                                                             >  2:146508/1‑251 (MQ=255)                                                                 gtgtTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGAAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTcc                                                                                                                                                                            >  1:252251/1‑250 (MQ=255)                                                                    ttgtgAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGc                                                                                                                                                                         >  1:430385/5‑250 (MQ=255)                                                                      gtgAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCAtc                                                                                                                                                                      >  1:347682/3‑251 (MQ=255)                                                                                aaaGATCTCCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGTGCTTTCGTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCGTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCAGGAAATTGTTCATATTCATAGGTTAACAAGCCATAAAGAGAGCCGAAAGTCTCCAGCATCTCTTTTGCCa                                                                                                                                                            <  1:217408/251‑1 (MQ=255)                                                                                     ccACCATAAATATCCTCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATCCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGtt                                                                                                                                                       <  2:309697/250‑1 (MQ=255)                                                                                         cATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATa                                                                                                                                                   <  1:13981/251‑1 (MQ=255)                                                                                                   tcCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTAcc                                                                                                                                         >  2:238422/1‑251 (MQ=255)                                                                                                             cACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTcc                                                                                                                                <  1:373656/250‑1 (MQ=255)                                                                                                             cACCCGTGAGCTGGCTTTGTAAAAACTCCCGCTACATCTCCTGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACGGGGCAAATTTCGCTACGCCAATTCCATTAACACCACTAAATTGTTCATATTCAGAGTTTAACAATCCATAAAGAGAGCCGAAATTCGCCAGCATCTCTTTTGCCAGGGTTAATACATCTGTACCGCGCGTTCCg                                                                                                                               <  2:177994/251‑1 (MQ=255)                                                                                                               cccGTGAGCTGGCTTTGTATAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGCACACCACTCACTTGTTCATATTCAGAGGTTAACAAGACATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATCCATCTTTACAGCGCGGTCCggg                                                                                                                             >  1:192974/1‑250 (MQ=255)                                                                                                                 cGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGt                                                                                                                                                                                                           >  2:173562/1‑171 (MQ=255)                                                                                                                 cGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGt                                                                                                                                                                                                           <  1:173562/171‑1 (MQ=255)                                                                                                                  gagaccTGGCTTTGAAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCCTCACCCATCCGCACTTTGTAGTAACGGCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTTCGCCAATTCCATGAACCCCCCTAAATTGTTCATATTCAGAGGTTACCAAGCCATAAAGAGAGCCGAAATTCTCCAGCAGCTCTTTTGCCAGTTTTAATACATCTTTACCGCGCGGTCCGGTACg                                                                                                                          <  2:252251/246‑1 (MQ=255)                                                                                                                                       tCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTc                                                                                                     >  2:162401/1‑251 (MQ=255)                                                                                                                                            cGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACAt                                                                                                <  1:82936/251‑1 (MQ=255)                                                                                                                                               cATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCCCGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTGCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCATCAGCTCTACATcc                                                                                              <  2:430385/250‑1 (MQ=255)                                                                                                                                                  ctcCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCCTGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAACTAGCGCCAGCAGCTCGCCATCCGtt                                                                                           >  1:228221/1‑250 (MQ=255)                                                                                                                                                      gggCTGATTAAAGGGCGTTCTCCACGCAGCCGCACTTGGTAGGAACGTCGCGCTAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATGGTCCATATTCAGAGTTTAACAAGCCATAAAGAGAGCCGAAATTCTCCATCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTGCCGGTTCGCAGAAATAGCGCCAGCAGCTCGTCAGCCGTTAAGgc                                                                                      <  2:207024/251‑1 (MQ=255)                                                                                                                                                         cTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGTTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCAAGCAGCTCGACATCCGTTCAGGAGCt                                                                                   >  1:437862/1‑251 (MQ=255)                                                                                                                                                            aGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAAt                                                                                <  1:230655/251‑1 (MQ=255)                                                                                                                                                               aaaGGGCTTTCTACACGCACCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCTCTACGCTAATTCCATGAACACCACAAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTGGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAACAGCTCGACATCCGTTAAGGCGCTAATAcc                                                                             <  1:279179/251‑1 (MQ=255)                                                                                                                                                                       ttcttcACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTc                                                                      >  2:182435/1‑250 (MQ=255)                                                                                                                                                                               gCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCAttttt                                                             >  1:237759/1‑251 (MQ=255)                                                                                                                                                                                                       gcgcCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCtgtgc                                      >  2:95765/1‑249 (MQ=255)                                                                                                                                                                                                            aGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGtt                                <  1:33782/251‑1 (MQ=255)                                                                                                                                                                                                               tCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGtttt                              <  2:143753/250‑1 (MQ=255)                                                                                                                                                                                                                    aaTCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTttcacct                        <  1:146508/251‑1 (MQ=255)                                                                                                                                                                                                                              aaCTGGGCAAGTTTCGCTACGCAAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGCACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTACGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTAACCTTCACCTCCtt              >  2:192066/1‑251 (MQ=255)                                                                                                                                                                                                                                  gggCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTtgtg          >  1:179604/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaaTTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGc      >  1:53699/1‑251 (MQ=255)                                                                                                                                                                                                                                           tCGCTACGCCAATTCCATGAACTCCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGCATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGc  <  1:224290/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      CCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCCACGCCAATTCCATGAACGCCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTTTCGCGCGGCATCAACAGCTGTGAATTGTTTTTCACCTTCACCTCCTTTGTGGTGCTCGC  >  NC_000913/3812100‑3812583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑