Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,812,296 C→T R88R (CGG→CGA)  yicR ← RadC‑like JAB domain‑containing protein YicR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,812,2960CT95.1% 111.1 / NA 41R88R (CGG→CGA) yicRRadC‑like JAB domain‑containing protein YicR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (23/16);  minor base G (0/2);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCCACGCCAATTCCATGAACGCCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTT  >  NC_000913/3812055‑3812524
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    
ggATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCGCCTCACCCGTGAGCTGGCTGTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTGCACGCATCCGCACGTTGTAGTAACGGCGCGCCAGt                                                                                                                                                                                                                             <  1:145267/251‑1 (MQ=255)
  aTCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTc                                                                                                                                                                                                                           <  2:29045/251‑1 (MQ=255)
     aGCGCCGCGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGc                                                                                                                                                                                                                        >  2:230655/1‑251 (MQ=255)
                      ttttATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTCGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTCGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTggg                                                                                                                                                                                                       >  1:146581/1‑251 (MQ=255)
                        ttATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTACCGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGc                                                                                                                                                                                                      >  2:337633/1‑250 (MQ=255)
                            cGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTACCGCGTCATCTCTGGGCCGAGTAAAGGGCTTTCTACACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAAtt                                                                                                                                                                                                 >  1:135618/1‑251 (MQ=255)
                              cTTCGCGGATAATTTCCCGAGGAAGGACTTCAACATGGGTTAGCGTGCCGGAAAAATGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCATGAATCCGCACGTTGTAGTAACGTCGCGACAGTTCAGCAATCCCCTTTAACTGGGCAAATTTc                                                                                                                                                                                               >  2:97381/1‑251 (MQ=255)
                               ttCGCGGATCATTTTCCGAGGATGGAATTCAGCATGGTTTAGTTTGCAGGAAAAAAGACGGCTATGCGTTATAACCCAGTGTTGTGAGTCGAGAAAGATCAACATACAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAATAATTCACGCGTCATCTCCGGGATGAGTACAGGGCTTTCTTCACGCCTCCGCAAGTTGTAGTAACGTCGCGCCAGTTCAGCAATACCCCTTAACTGGGCAAATTTcg                                                                                                                                                                                              >  2:176346/1‑251 (MQ=255)
                                             cccGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTcc                                                                                                                                                                                 >  1:321576/1‑250 (MQ=255)
                                              ccGAGGATGGACTTCAACATGGGTGAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCGCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTGTCTTCACGCAGCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCAt                                                                                                                                                                               <  1:181557/251‑1 (MQ=255)
                                                                  gggttAGCGTGCCGGCAACAAACCGGCTATGCGTTATACCCCGGAGTTGTGCGTCGAGAAAGCTAAACATAACGATCTACCGCTCCTCACCCGTGAGCTCGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGAATCCGCACGTTGTCGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAAATGTTc                                                                                                                                                            >  2:268701/4‑250 (MQ=255)
                                                                  ggTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTc                                                                                                                                                            >  1:143753/1‑250 (MQ=255)
                                                                              cGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTc                                                                                                                                                                                  <  1:157397/216‑1 (MQ=255)
                                                                              cGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTc                                                                                                                                                                                  >  2:157397/1‑216 (MQ=255)
                                                                                              aTGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAagag                                                                                                                               <  1:273905/251‑1 (MQ=255)
                                                                                                 cGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCACTCCCCTTTAACTGGGCAACTTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACACGCCATAAAGAGAGc                                                                                                                            >  1:222239/1‑251 (MQ=255)
                                                                                                      taacccggtggtttggtgggagtaaGACCACCATAAAGATCTCCCGCACCGAACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCGTCTGGCTGTGTCAAGGGCTTTCTACGCGCTTCGACGCGTTGTAGGATCCTCTCGCCAGTTCAGCAGACCCCTTAAACGGGTCTAATTTCGCTACGCCAATGCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGaa                                                                                                                        <  1:176346/227‑1 (MQ=255)
                                                                                                           cGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATtctc                                                                                                                  >  2:146508/1‑251 (MQ=255)
                                                                                                             gtgtTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGAAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTcc                                                                                                                 >  1:252251/1‑250 (MQ=255)
                                                                                                                ttgtgAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGc                                                                                                              >  1:430385/5‑250 (MQ=255)
                                                                                                                  gtgAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCAtc                                                                                                           >  1:347682/3‑251 (MQ=255)
                                                                                                                            aaaGATCTCCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGTGCTTTCGTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCGTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCAGGAAATTGTTCATATTCATAGGTTAACAAGCCATAAAGAGAGCCGAAAGTCTCCAGCATCTCTTTTGCCa                                                                                                 <  1:217408/251‑1 (MQ=255)
                                                                                                                                 ccACCATAAATATCCTCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATCCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGtt                                                                                            <  2:309697/250‑1 (MQ=255)
                                                                                                                                     cATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATa                                                                                        <  1:13981/251‑1 (MQ=255)
                                                                                                                                               tcCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTAcc                                                                              >  2:238422/1‑251 (MQ=255)
                                                                                                                                                         cACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTcc                                                                     <  1:373656/250‑1 (MQ=255)
                                                                                                                                                         cACCCGTGAGCTGGCTTTGTAAAAACTCCCGCTACATCTCCTGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACGGGGCAAATTTCGCTACGCCAATTCCATTAACACCACTAAATTGTTCATATTCAGAGTTTAACAATCCATAAAGAGAGCCGAAATTCGCCAGCATCTCTTTTGCCAGGGTTAATACATCTGTACCGCGCGTTCCg                                                                    <  2:177994/251‑1 (MQ=255)
                                                                                                                                                           cccGTGAGCTGGCTTTGTATAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGCACACCACTCACTTGTTCATATTCAGAGGTTAACAAGACATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATCCATCTTTACAGCGCGGTCCggg                                                                  >  1:192974/1‑250 (MQ=255)
                                                                                                                                                             cGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGt                                                                                                                                                >  2:173562/1‑171 (MQ=255)
                                                                                                                                                             cGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGt                                                                                                                                                <  1:173562/171‑1 (MQ=255)
                                                                                                                                                              gagaccTGGCTTTGAAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCCTCACCCATCCGCACTTTGTAGTAACGGCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTTCGCCAATTCCATGAACCCCCCTAAATTGTTCATATTCAGAGGTTACCAAGCCATAAAGAGAGCCGAAATTCTCCAGCAGCTCTTTTGCCAGTTTTAATACATCTTTACCGCGCGGTCCGGTACg                                                               <  2:252251/246‑1 (MQ=255)
                                                                                                                                                                                   tCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTc                                          >  2:162401/1‑251 (MQ=255)
                                                                                                                                                                                        cGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACAt                                     <  1:82936/251‑1 (MQ=255)
                                                                                                                                                                                           cATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCCCGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTGCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCATCAGCTCTACATcc                                   <  2:430385/250‑1 (MQ=255)
                                                                                                                                                                                              ctcCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCCTGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAACTAGCGCCAGCAGCTCGCCATCCGtt                                >  1:228221/1‑250 (MQ=255)
                                                                                                                                                                                                  gggCTGATTAAAGGGCGTTCTCCACGCAGCCGCACTTGGTAGGAACGTCGCGCTAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATGGTCCATATTCAGAGTTTAACAAGCCATAAAGAGAGCCGAAATTCTCCATCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTGCCGGTTCGCAGAAATAGCGCCAGCAGCTCGTCAGCCGTTAAGgc                           <  2:207024/251‑1 (MQ=255)
                                                                                                                                                                                                     cTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGTTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCAAGCAGCTCGACATCCGTTCAGGAGCt                        >  1:437862/1‑251 (MQ=255)
                                                                                                                                                                                                        aGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAAt                     <  1:230655/251‑1 (MQ=255)
                                                                                                                                                                                                           aaaGGGCTTTCTACACGCACCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCTCTACGCTAATTCCATGAACACCACAAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTGGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAACAGCTCGACATCCGTTAAGGCGCTAATAcc                  <  1:279179/251‑1 (MQ=255)
                                                                                                                                                                                                                   ttcttcACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTc           >  2:182435/1‑250 (MQ=255)
                                                                                                                                                                                                                           gCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCAttttt  >  1:237759/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    
GGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCCACGCCAATTCCATGAACGCCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCCAGCATCTCTTTTGCCAGGGTTAATACATCTTTACCGCGCGTTCCGGTACGCAGAAATAGCGCCAGCAGCTCGACATCCGTTAAGGCGCTAATACCAAACTTCAGCATTTTT  >  NC_000913/3812055‑3812524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑