Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,812,170 G→T S130S (TCC→TCA)  yicR ← RadC‑like JAB domain‑containing protein YicR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,812,1700GT94.1% 109.4 / NA 34S130S (TCC→TCA) yicRRadC‑like JAB domain‑containing protein YicR
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (21/11);  total (22/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCCACGCCAATTCCATGAACGCCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCC  >  NC_000913/3811926‑3812413
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   
ctcCACGCACAACCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGCTCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCg                                                                                                                                                                                                                                               >  2:224290/1‑251 (MQ=255)
 tcCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCAGGAACTGCCAACTCTTTATTATCCGTTCAGTAATGAGTTTCTCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATAAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCgg                                                                                                                                                                                                                                              >  1:207024/1‑250 (MQ=255)
  ccACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCTAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGAGGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCgag                                                                                                                                                                                                                                             <  1:293267/251‑1 (MQ=255)
  ccACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTc                                                                                                                                                                                                                                                <  1:273239/248‑1 (MQ=255)
   cACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCg                                                                                                                                                                                                                                               >  1:103838/1‑248 (MQ=255)
                      gTCGAGCACGCGTAAATCCATGAACTTAAAACTCTTTAGTATCCTTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCAGATCCCGAAGGGTGATTATGTGCGAGGATCAGCGCCGAGGTGTGTATTTTTAGCGCGTCGCGGATAATTTTCCGAGTATGGACTTCAACATCGTTTAGCGGGCCGGAAAAAAGACGCCGATGCCGTATAACCCGGTGTTGTGAGTCGAGAAAGATCAACATAAAGAtct                                                                                                                                                                                                                         <  1:412057/251‑1 (MQ=255)
                      gTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGAtct                                                                                                                                                                                                                         >  2:273905/1‑251 (MQ=255)
                       tCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTCTCCGTTCCGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGAtctc                                                                                                                                                                                                                        >  2:32263/1‑251 (MQ=255)
                                        cATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTg                                                                                                                                                                                                        >  1:31970/1‑250 (MQ=255)
                                           gAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGc                                                                                                                                                                                                     <  2:118192/250‑1 (MQ=255)
                                                                             aGTTTATCCGCTTTACTGGGTTCAGCACATCCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCt                                                                                                                                                                  <  1:410968/251‑1 (MQ=255)
                                                                                              tggTTCAGCACAACCCGAAGGTTGATTATGTGCAAGGATCAGCGCCGAGGCTTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTttc                                                                                                                                                   <  2:237426/248‑1 (MQ=255)
                                                                                                              gAAGGGTGATTATTTGCCTGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGCTAATTTCCCGAGGTTGGCCTTCAACATGGTTTAGCGTGCCGGAAACAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGATTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGtt                                                                                                                                 >  2:181557/1‑251 (MQ=255)
                                                                                                                  ggTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTgtag                                                                                                                             <  1:181852/251‑1 (MQ=255)
                                                                                                                       ttATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAAcg                                                                                                                        >  2:111213/1‑251 (MQ=255)
                                                                                                                       ttATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAAcg                                                                                                                        >  1:309697/1‑251 (MQ=255)
                                                                                                                                 ggATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCGCCTCACCCGTGAGCTGGCTGTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTGCACGCATCCGCACGTTGTAGTAACGGCGCGCCAGt                                                                                                              <  1:145267/251‑1 (MQ=255)
                                                                                                                                   aTCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTc                                                                                                            <  2:29045/251‑1 (MQ=255)
                                                                                                                                      aGCGCCGCGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGc                                                                                                         >  2:230655/1‑251 (MQ=255)
                                                                                                                                                       ttttATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTCGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTCGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTggg                                                                                        >  1:146581/1‑251 (MQ=255)
                                                                                                                                                         ttATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTACCGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGc                                                                                       >  2:337633/1‑250 (MQ=255)
                                                                                                                                                             cGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTACCGCGTCATCTCTGGGCCGAGTAAAGGGCTTTCTACACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAAtt                                                                                  >  1:135618/1‑251 (MQ=255)
                                                                                                                                                               cTTCGCGGATAATTTCCCGAGGAAGGACTTCAACATGGGTTAGCGTGCCGGAAAAATGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCATGAATCCGCACGTTGTAGTAACGTCGCGACAGTTCAGCAATCCCCTTTAACTGGGCAAATTTc                                                                                >  2:97381/1‑251 (MQ=255)
                                                                                                                                                                ttCGCGGATCATTTTCCGAGGATGGAATTCAGCATGGTTTAGTTTGCAGGAAAAAAGACGGCTATGCGTTATAACCCAGTGTTGTGAGTCGAGAAAGATCAACATACAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAATAATTCACGCGTCATCTCCGGGATGAGTACAGGGCTTTCTTCACGCCTCCGCAAGTTGTAGTAACGTCGCGCCAGTTCAGCAATACCCCTTAACTGGGCAAATTTcg                                                                               >  2:176346/1‑251 (MQ=255)
                                                                                                                                                                              cccGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTcc                                                                  >  1:321576/1‑250 (MQ=255)
                                                                                                                                                                               ccGAGGATGGACTTCAACATGGGTGAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCGCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTGTCTTCACGCAGCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCAt                                                                <  1:181557/251‑1 (MQ=255)
                                                                                                                                                                                                   gggttAGCGTGCCGGCAACAAACCGGCTATGCGTTATACCCCGGAGTTGTGCGTCGAGAAAGCTAAACATAACGATCTACCGCTCCTCACCCGTGAGCTCGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGAATCCGCACGTTGTCGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAAATGTTc                                             >  2:268701/4‑250 (MQ=255)
                                                                                                                                                                                                   ggTTTAGCGTGCCGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTc                                             >  1:143753/1‑250 (MQ=255)
                                                                                                                                                                                                               cGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTc                                                                   >  2:157397/1‑216 (MQ=255)
                                                                                                                                                                                                               cGGAAAAAAGACGGCTATGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTc                                                                   <  1:157397/216‑1 (MQ=255)
                                                                                                                                                                                                                               aTGCGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAagag                <  1:273905/251‑1 (MQ=255)
                                                                                                                                                                                                                                  cGTTATAACCCGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCACTCCCCTTTAACTGGGCAACTTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACACGCCATAAAGAGAGc             >  1:222239/1‑251 (MQ=255)
                                                                                                                                                                                                                                            cGGTGTTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATtctc   >  2:146508/1‑251 (MQ=255)
                                                                                                                                                                                                                                              gtgtTGTGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCGCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGTCGCGCCAGTTCAGCAATCCCCTTTAACTGGGAAAATTTCGCTACGCCAATTCCATGAACACCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTcc  >  1:252251/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   
CTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTGGGCAAATTTCGCCACGCCAATTCCATGAACGCCACTAAATTGTTCATATTCAGAGGTTAACAAGCCATAAAGAGAGCCGAAATTCTCC  >  NC_000913/3811926‑3812413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑