Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,811,120	G→A	R11R (CGC→CGT)	mutM ←	DNA‑formamidopyrimidine glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,811,120	0	G	A	96.8%	92.2 / NA	31	R11R (CGC→CGT)	mutM	DNA‑formamidopyrimidine glycosylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/16);  minor base T (0/1);  total (14/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ATGGTCATGCTTTTCAGGGGGAAGTTCTTCTGGAAGGATGCGCAGGCTGCCAGACATCCCTAAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTC  >  NC_000913/3810877‑3811343                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                aTGGTCATGCTTTTCAGGGGGAAGTTCTTCTGGAAGGATGCGCAGGCTGCCAGACATCCCCAAATGAATGATAACCCAGCCCTCAGGCAGCGCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTgg                                                                                                                                                                                                                          <  1:248893/251‑1 (MQ=255) aTGGTCAAGCTTTTCAGGGGGAAGTTCTTCTGGAAGGATGCGCAGGCTGCCAGACATCCCCAAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGTTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTGGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTgg                                                                                                                                                                                                                          >  2:61858/1‑251 (MQ=255)      cATGCTTTTGAGGGGGAAGTTCTTCTGGAAGGATGCGCAGGCTGCCCGACATCAACACATGATTGATAATCCAGCCATCAGGCAGCTCCCGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGCTGCTCGATTAAACGGTAGATCTCTTATGACACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGCTGCGGTTCTCTGCCGCGACGGCTGGGTTc                                                                                                                                                                                                                      >  2:410146/1‑250 (MQ=255)             ttCAGGGGGAAGTTCTTCTGGAAGGATGCGCAGGCTGCCAGACATCCCCAAAGGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCg                                                                                                                                                                                                               <  1:284033/250‑1 (MQ=255)                    ggAAGTTCTTCTGGAAGGATGCGCAGCCTGCCAGACATCCCCAAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTc                                                                                                                                                                                                       <  1:157232/251‑1 (MQ=255)                       aGTTCTTCTGGAAGGATGCGCAGGCTGCCAGACATCCCCAAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAgg                                                                                                                                                                                                    <  1:52432/251‑1 (MQ=255)                                ggAAGGATGCGCAGGCTGCCAGACATCCCCAAATGACTGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCAtc                                                                                                                                                                                           >  2:166682/1‑251 (MQ=255)                                                 gCCAGACATCCCCAAATGAAGGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCGTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCTCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGTCATAGCATCTCCAGGAATGAACAGAt                                                                                                                                                                          <  2:162658/251‑1 (MQ=255)                                                 cccaaaCATCCCCAAATGACTGATCACCCTGCCCTGAGCCAGCTCCAGCGTCAGATATTTAGCCCGCCTCTGCACGCTAAGCACTGGTTGCTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCCCCACCGCATGAAGAATGGTTGCACCAACGAGAAGCGGGTCTATGCCTCGACGGCTGGTTTCAACTTCGGGTAAATCAGGCATAGCATCTCCAGGAATGAACAGAt                                                                                                                                                                          <  2:385339/246‑1 (MQ=255)                                                      aCATCCCCAAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCaat                                                                                                                                                                     >  2:267725/1‑249 (MQ=255)                                                         cccccaaaTGAATGATAACCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTATCCCGCCGCTGCACGCGAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGGTCTATGCCGCGTCGGCTGGTTTCAACGTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGctatcaa                                                                                                                                                                  <  2:30738/246‑7 (MQ=255)                                                               aatgactgATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCCCTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCACCTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATggg                                                                                                                                                            >  2:194036/1‑251 (MQ=255)                                                                       aTAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGACCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCACCGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGc                                                                                                                                                     >  1:82698/1‑250 (MQ=255)                                                                              aGCCCTCAGGCAGCTCCAGCAGAAGATATTGAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACa                                                                                                                                             <  2:274822/251‑1 (MQ=255)                                                                               ccccTCAGGCATCCCCATCAGTATATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCGCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTTGTTTCATCTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACaa                                                                                                                                            <  1:179839/250‑1 (MQ=255)                                                                                         aGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCccgc                                                                                                                                  <  1:166682/251‑1 (MQ=255)                                                                                                    cagctatatAGCCCGCCGCTGCACGCTAAGCACTGGTTGCTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCCGCGCAAGCGTCCGTTGCGCCCCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCACCTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGa                                                                                                                                                  >  2:181617/9‑224 (MQ=255)                                                                                                    cagATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCGTCTGAAACCGGCCAGCGCAAGCGGCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAGCGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGa                                                                                                                                                  <  1:181617/224‑1 (MQ=255)                                                                                                    cagATATTTAGCCCGCAGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGCGCGAGGt                                                                                                                       >  1:320817/1‑251 (MQ=255)                                                                                                     agATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtt                                                                                                                      <  2:108340/251‑1 (MQ=255)                                                                                                     agATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtt                                                                                                                      <  1:257528/251‑1 (MQ=255)                                                                                                                               aagctcttgtttgtctcTTAAACGGTAGTTCTCTGTTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCCCCTCCGAATGAAGAATGGTTGCACCACCGAGATGCGTCTCTATGCCGCGACGGATGTTGTCAACTTCTGGTAATCCAGGCATATCATCCCCAGGAATGAACAGATTCACTCAATATGGGTGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTCGTTACATCAAAGCGAGAattat                                                                                            <  2:421395/235‑1 (MQ=255)                                                                                                                                  cACTGGTTGGTCGCTTAATAGGTAGCTCTCTTCTGATACCGGCCCGCGCAAGCCTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCCTAGAATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTCGTTACATCAAAGCGAGAATTATTTg                                                                                         >  2:357638/1‑251 (MQ=255)                                                                                                                                       gttggtCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTCGTTACATCAAAGCGAGAATTATTTGAtttt                                                                                    >  1:300324/1‑251 (MQ=255)                                                                                                                                                 tAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTCGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCtttt                                                                          >  2:3013/1‑250 (MQ=255)                                                                                                                                                       gTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTCGTTACATCAAAGCGAGAATTATTTGATGTTCGCTTCTTTGTAGATc                                                                    <  1:112050/251‑1 (MQ=255)                                                                                                                                                         agatcgcgtctGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTCTTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCAc                                                                  <  1:422956/243‑1 (MQ=255)                                                                                                                                                                         cGGCCAGCGCAAGCGTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACAAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCCACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTCGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACt                                                  >  1:187623/1‑251 (MQ=255)                                                                                                                                                                                       gTCCGTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTCGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttttt                                    >  1:38519/1‑251 (MQ=255)                                                                                                                                                                                           gTTGCGCACCACCGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCCGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTCGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGt                                >  2:142288/1‑251 (MQ=255)                                                                                                                                                                                                                         aCCAACGAGATGCGGTTCTATGCCGCGACGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAATCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTCGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGttc  <  1:184659/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                ATGGTCATGCTTTTCAGGGGGAAGTTCTTCTGGAAGGATGCGCAGGCTGCCAGACATCCCTAAATGAATGATAATCCAGCCCTCAGGCAGCTCCAGCAGCAGATATTTAGCCCGCCGCTGCACGCTAAGCACTGGTTGGTCGCTTAAACGGTAGATCTCTTCTGAAACCGGCCAGCGCAAGCGTCCGTTGCGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTC  >  NC_000913/3810877‑3811343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑